Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20075 and BWI76_RS20080 are separated by 77 nucleotides BWI76_RS20080 and BWI76_RS20085 are separated by 67 nucleotides BWI76_RS20085 and BWI76_RS20090 are separated by 60 nucleotides
BWI76_RS20075: BWI76_RS20075 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 4,205,196 to 4,206,866
_RS20075
BWI76_RS20080: BWI76_RS20080 - isochorismate synthase MenF, at 4,206,944 to 4,208,269
_RS20080
BWI76_RS20085: BWI76_RS20085 - GNAT family N-acetyltransferase, at 4,208,337 to 4,208,798
_RS20085
BWI76_RS20090: BWI76_RS20090 - ribonuclease Z, at 4,208,859 to 4,209,776
_RS20090
Position (kb) 4206
4207
4208
4209 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4206.257 kb on - strand, within BWI76_RS20075 at 4206.268 kb on + strand, within BWI76_RS20075 at 4206.414 kb on + strand, within BWI76_RS20075 at 4206.612 kb on - strand, within BWI76_RS20075 at 4206.761 kb on - strand at 4206.830 kb on + strand at 4206.845 kb on + strand at 4206.904 kb on - strand at 4207.023 kb on - strand at 4207.563 kb on - strand, within BWI76_RS20080 at 4207.664 kb on + strand, within BWI76_RS20080 at 4207.671 kb on + strand, within BWI76_RS20080 at 4207.872 kb on - strand, within BWI76_RS20080 at 4207.911 kb on - strand, within BWI76_RS20080 at 4207.911 kb on - strand, within BWI76_RS20080 at 4208.076 kb on + strand, within BWI76_RS20080 at 4208.122 kb on + strand, within BWI76_RS20080 at 4208.203 kb on + strand at 4208.408 kb on + strand, within BWI76_RS20085 at 4208.525 kb on - strand, within BWI76_RS20085 at 4208.743 kb on + strand, within BWI76_RS20085 at 4208.799 kb on - strand at 4208.923 kb on - strand at 4209.076 kb on + strand, within BWI76_RS20090 at 4209.110 kb on - strand, within BWI76_RS20090 at 4209.144 kb on - strand, within BWI76_RS20090 at 4209.223 kb on + strand, within BWI76_RS20090
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 4,206,257 - BWI76_RS20075 0.63 -0.0 4,206,268 + BWI76_RS20075 0.64 +0.6 4,206,414 + BWI76_RS20075 0.73 -0.6 4,206,612 - BWI76_RS20075 0.85 -1.9 4,206,761 - +0.3 4,206,830 + +0.4 4,206,845 + -2.4 4,206,904 - -0.6 4,207,023 - -0.5 4,207,563 - BWI76_RS20080 0.47 +1.2 4,207,664 + BWI76_RS20080 0.54 +1.2 4,207,671 + BWI76_RS20080 0.55 +1.3 4,207,872 - BWI76_RS20080 0.70 +0.3 4,207,911 - BWI76_RS20080 0.73 +0.1 4,207,911 - BWI76_RS20080 0.73 +0.9 4,208,076 + BWI76_RS20080 0.85 +2.1 4,208,122 + BWI76_RS20080 0.89 -0.4 4,208,203 + +1.4 4,208,408 + BWI76_RS20085 0.15 +0.9 4,208,525 - BWI76_RS20085 0.41 +0.6 4,208,743 + BWI76_RS20085 0.88 +0.7 4,208,799 - -0.4 4,208,923 - +0.7 4,209,076 + BWI76_RS20090 0.24 +0.1 4,209,110 - BWI76_RS20090 0.27 +0.2 4,209,144 - BWI76_RS20090 0.31 +2.0 4,209,223 + BWI76_RS20090 0.40 +1.1
Or see this region's nucleotide sequence