Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17800

Experiment: KLEB20 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17795 and BWI76_RS17800 are separated by 69 nucleotidesBWI76_RS17800 and BWI76_RS17805 are separated by 175 nucleotidesBWI76_RS17805 and BWI76_RS17810 are separated by 43 nucleotides BWI76_RS17795: BWI76_RS17795 - ribonuclease D, at 3,697,932 to 3,699,059 _RS17795 BWI76_RS17800: BWI76_RS17800 - long-chain-fatty-acid--CoA ligase, at 3,699,129 to 3,700,847 _RS17800 BWI76_RS17805: BWI76_RS17805 - membrane protein, at 3,701,023 to 3,701,607 _RS17805 BWI76_RS17810: BWI76_RS17810 - tRNA N6-adenosine(37)-N6-threonylcarbamoyltransferase complex dimerization subunit TsaB, at 3,701,651 to 3,702,346 _RS17810 Position (kb) 3699 3700 3701Strain fitness (log2 ratio) -3 -2 -1 0 1at 3698.670 kb on - strand, within BWI76_RS17795at 3698.717 kb on - strand, within BWI76_RS17795at 3698.939 kb on + strand, within BWI76_RS17795at 3699.781 kb on - strand, within BWI76_RS17800at 3699.888 kb on - strand, within BWI76_RS17800at 3699.940 kb on - strand, within BWI76_RS17800at 3700.104 kb on - strand, within BWI76_RS17800at 3700.396 kb on - strand, within BWI76_RS17800at 3700.497 kb on - strand, within BWI76_RS17800at 3700.547 kb on + strand, within BWI76_RS17800at 3700.716 kb on + strandat 3701.461 kb on - strand, within BWI76_RS17805

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1
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3,698,670 - BWI76_RS17795 0.65 +0.1
3,698,717 - BWI76_RS17795 0.70 +0.9
3,698,939 + BWI76_RS17795 0.89 -3.4
3,699,781 - BWI76_RS17800 0.38 -2.1
3,699,888 - BWI76_RS17800 0.44 -2.4
3,699,940 - BWI76_RS17800 0.47 -0.1
3,700,104 - BWI76_RS17800 0.57 -0.5
3,700,396 - BWI76_RS17800 0.74 -3.1
3,700,497 - BWI76_RS17800 0.80 -0.6
3,700,547 + BWI76_RS17800 0.82 -1.1
3,700,716 + +1.6
3,701,461 - BWI76_RS17805 0.75 -0.9

Or see this region's nucleotide sequence