Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16705 and BWI76_RS16710 are separated by 496 nucleotides BWI76_RS16710 and BWI76_RS16715 are separated by 66 nucleotides BWI76_RS16715 and BWI76_RS16720 are separated by 725 nucleotides
BWI76_RS16705: BWI76_RS16705 - glucose dehydrogenase, at 3,457,562 to 3,459,967
_RS16705
BWI76_RS16710: BWI76_RS16710 - cyclic nucleotide-binding protein, at 3,460,464 to 3,460,693
_RS16710
BWI76_RS16715: BWI76_RS16715 - hypothetical protein, at 3,460,760 to 3,461,668
_RS16715
BWI76_RS16720: BWI76_RS16720 - hypothetical protein, at 3,462,394 to 3,463,731
_RS16720
Position (kb)
3460
3461
3462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 3459.771 kb on - strand at 3459.803 kb on - strand at 3459.847 kb on - strand at 3460.060 kb on - strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.252 kb on + strand at 3460.258 kb on - strand at 3460.354 kb on + strand at 3460.391 kb on - strand at 3460.416 kb on + strand at 3460.419 kb on + strand at 3460.424 kb on - strand at 3460.424 kb on - strand at 3460.470 kb on - strand at 3460.481 kb on - strand at 3460.520 kb on + strand, within BWI76_RS16710 at 3460.528 kb on - strand at 3460.637 kb on - strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.664 kb on - strand at 3460.693 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.725 kb on + strand at 3460.728 kb on - strand at 3460.733 kb on - strand at 3460.812 kb on - strand at 3460.813 kb on - strand at 3460.821 kb on - strand at 3460.830 kb on - strand at 3460.831 kb on - strand at 3460.831 kb on - strand at 3460.863 kb on + strand at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.881 kb on - strand, within BWI76_RS16715 at 3460.920 kb on + strand at 3460.975 kb on - strand, within BWI76_RS16715 at 3461.054 kb on - strand, within BWI76_RS16715 at 3461.054 kb on - strand, within BWI76_RS16715 at 3461.085 kb on + strand, within BWI76_RS16715 at 3461.093 kb on - strand, within BWI76_RS16715 at 3461.093 kb on - strand, within BWI76_RS16715 at 3461.093 kb on - strand, within BWI76_RS16715 at 3461.201 kb on - strand, within BWI76_RS16715 at 3461.219 kb on + strand, within BWI76_RS16715 at 3461.219 kb on + strand, within BWI76_RS16715 at 3461.227 kb on - strand, within BWI76_RS16715 at 3461.266 kb on + strand, within BWI76_RS16715 at 3461.272 kb on + strand, within BWI76_RS16715 at 3461.272 kb on + strand, within BWI76_RS16715 at 3461.280 kb on - strand, within BWI76_RS16715 at 3461.280 kb on - strand, within BWI76_RS16715 at 3461.313 kb on - strand, within BWI76_RS16715 at 3461.326 kb on - strand, within BWI76_RS16715 at 3461.326 kb on - strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.398 kb on - strand, within BWI76_RS16715 at 3461.398 kb on - strand, within BWI76_RS16715 at 3461.442 kb on + strand, within BWI76_RS16715 at 3461.482 kb on - strand, within BWI76_RS16715 at 3461.497 kb on - strand, within BWI76_RS16715 at 3461.512 kb on - strand, within BWI76_RS16715 at 3461.562 kb on - strand, within BWI76_RS16715 at 3461.604 kb on - strand at 3461.655 kb on - strand at 3461.655 kb on - strand at 3461.673 kb on - strand at 3461.738 kb on - strand at 3461.868 kb on - strand at 3461.883 kb on + strand at 3461.883 kb on + strand at 3461.883 kb on + strand at 3461.883 kb on + strand at 3461.918 kb on - strand at 3461.923 kb on - strand at 3461.930 kb on + strand at 3462.033 kb on + strand at 3462.083 kb on + strand at 3462.306 kb on - strand at 3462.322 kb on + strand at 3462.498 kb on + strand at 3462.621 kb on + strand, within BWI76_RS16720 at 3462.629 kb on - strand, within BWI76_RS16720
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 3,459,771 - +3.0 3,459,803 - -0.6 3,459,847 - +2.0 3,460,060 - +0.9 3,460,072 + +0.0 3,460,072 + -0.8 3,460,072 + +2.2 3,460,072 + +1.4 3,460,072 + +1.8 3,460,072 + -0.0 3,460,080 - -2.8 3,460,080 - +0.7 3,460,080 - -0.7 3,460,080 - -1.2 3,460,252 + +2.8 3,460,258 - +0.9 3,460,354 + -0.8 3,460,391 - +0.5 3,460,416 + +0.7 3,460,419 + +1.8 3,460,424 - +0.6 3,460,424 - +1.0 3,460,470 - +0.5 3,460,481 - +0.9 3,460,520 + BWI76_RS16710 0.24 +2.0 3,460,528 - +0.9 3,460,637 - BWI76_RS16710 0.75 +0.6 3,460,647 + BWI76_RS16710 0.80 -0.8 3,460,647 + BWI76_RS16710 0.80 +1.5 3,460,647 + BWI76_RS16710 0.80 -2.5 3,460,647 + BWI76_RS16710 0.80 -0.2 3,460,647 + BWI76_RS16710 0.80 -1.6 3,460,647 + BWI76_RS16710 0.80 +0.3 3,460,647 + BWI76_RS16710 0.80 -0.6 3,460,647 + BWI76_RS16710 0.80 -0.2 3,460,655 - BWI76_RS16710 0.83 +0.5 3,460,655 - BWI76_RS16710 0.83 -3.6 3,460,655 - BWI76_RS16710 0.83 -1.0 3,460,655 - BWI76_RS16710 0.83 -0.2 3,460,655 - BWI76_RS16710 0.83 -0.0 3,460,655 - BWI76_RS16710 0.83 +0.5 3,460,655 - BWI76_RS16710 0.83 -1.0 3,460,655 - BWI76_RS16710 0.83 +0.4 3,460,655 - BWI76_RS16710 0.83 -0.8 3,460,655 - BWI76_RS16710 0.83 +1.6 3,460,655 - BWI76_RS16710 0.83 -0.1 3,460,664 - +0.2 3,460,693 + -1.2 3,460,698 + +0.6 3,460,698 + +0.3 3,460,698 + +1.3 3,460,698 + +1.8 3,460,698 + +1.6 3,460,725 + -0.6 3,460,728 - +5.8 3,460,733 - +4.6 3,460,812 - +6.6 3,460,813 - +6.2 3,460,821 - +6.6 3,460,830 - +6.3 3,460,831 - +5.2 3,460,831 - +5.5 3,460,863 + +6.3 3,460,871 - BWI76_RS16715 0.12 +6.2 3,460,871 - BWI76_RS16715 0.12 +6.4 3,460,871 - BWI76_RS16715 0.12 +4.8 3,460,881 - BWI76_RS16715 0.13 +5.6 3,460,920 + +3.6 3,460,975 - BWI76_RS16715 0.24 +6.1 3,461,054 - BWI76_RS16715 0.32 +5.3 3,461,054 - BWI76_RS16715 0.32 +6.4 3,461,085 + BWI76_RS16715 0.36 +5.5 3,461,093 - BWI76_RS16715 0.37 +6.9 3,461,093 - BWI76_RS16715 0.37 +6.5 3,461,093 - BWI76_RS16715 0.37 +6.4 3,461,201 - BWI76_RS16715 0.49 +6.1 3,461,219 + BWI76_RS16715 0.50 +6.2 3,461,219 + BWI76_RS16715 0.50 +7.3 3,461,227 - BWI76_RS16715 0.51 +6.4 3,461,266 + BWI76_RS16715 0.56 +5.1 3,461,272 + BWI76_RS16715 0.56 +6.6 3,461,272 + BWI76_RS16715 0.56 -0.2 3,461,280 - BWI76_RS16715 0.57 +6.0 3,461,280 - BWI76_RS16715 0.57 +7.1 3,461,313 - BWI76_RS16715 0.61 +6.3 3,461,326 - BWI76_RS16715 0.62 +6.0 3,461,326 - BWI76_RS16715 0.62 +6.4 3,461,334 + BWI76_RS16715 0.63 +5.9 3,461,334 + BWI76_RS16715 0.63 +6.8 3,461,334 + BWI76_RS16715 0.63 +6.0 3,461,334 + BWI76_RS16715 0.63 +7.5 3,461,342 - BWI76_RS16715 0.64 +5.0 3,461,342 - BWI76_RS16715 0.64 +5.5 3,461,342 - BWI76_RS16715 0.64 +5.6 3,461,342 - BWI76_RS16715 0.64 +5.6 3,461,342 - BWI76_RS16715 0.64 +6.1 3,461,398 - BWI76_RS16715 0.70 +5.5 3,461,398 - BWI76_RS16715 0.70 +5.9 3,461,442 + BWI76_RS16715 0.75 +0.2 3,461,482 - BWI76_RS16715 0.79 +6.2 3,461,497 - BWI76_RS16715 0.81 +6.0 3,461,512 - BWI76_RS16715 0.83 +5.8 3,461,562 - BWI76_RS16715 0.88 +4.6 3,461,604 - +5.0 3,461,655 - +5.8 3,461,655 - +6.1 3,461,673 - +5.2 3,461,738 - +4.1 3,461,868 - +4.8 3,461,883 + +3.7 3,461,883 + +3.9 3,461,883 + +2.4 3,461,883 + +3.7 3,461,918 - +3.4 3,461,923 - +3.6 3,461,930 + +3.2 3,462,033 + +1.3 3,462,083 + +1.5 3,462,306 - +1.5 3,462,322 + +0.9 3,462,498 + +0.5 3,462,621 + BWI76_RS16720 0.17 -2.4 3,462,629 - BWI76_RS16720 0.18 +1.5
Or see this region's nucleotide sequence