Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14205

Experiment: KLEB20 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14200 and BWI76_RS14205 are separated by 13 nucleotidesBWI76_RS14205 and BWI76_RS14210 are separated by 181 nucleotides BWI76_RS14200: BWI76_RS14200 - protocatechuate 4,5-dioxygenase, at 2,971,835 to 2,973,097 _RS14200 BWI76_RS14205: BWI76_RS14205 - aromatic acid/H+ symport family MFS transporter, at 2,973,111 to 2,974,480 _RS14205 BWI76_RS14210: BWI76_RS14210 - LysR family transcriptional regulator, at 2,974,662 to 2,975,624 _RS14210 Position (kb) 2973 2974 2975Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2972.572 kb on - strand, within BWI76_RS14200at 2972.596 kb on + strand, within BWI76_RS14200at 2972.635 kb on - strand, within BWI76_RS14200at 2973.711 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.745 kb on + strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.753 kb on - strand, within BWI76_RS14205at 2973.866 kb on + strand, within BWI76_RS14205at 2973.997 kb on - strand, within BWI76_RS14205at 2974.002 kb on - strand, within BWI76_RS14205at 2974.027 kb on + strand, within BWI76_RS14205at 2974.056 kb on + strand, within BWI76_RS14205at 2974.056 kb on + strand, within BWI76_RS14205at 2974.197 kb on - strand, within BWI76_RS14205at 2974.284 kb on - strand, within BWI76_RS14205at 2974.349 kb on + strandat 2974.349 kb on + strandat 2974.349 kb on + strandat 2974.349 kb on + strandat 2974.349 kb on + strandat 2974.357 kb on - strandat 2974.357 kb on - strandat 2974.367 kb on - strandat 2974.371 kb on + strandat 2974.402 kb on + strandat 2974.405 kb on + strandat 2974.413 kb on - strandat 2974.442 kb on + strandat 2974.450 kb on + strandat 2974.476 kb on + strandat 2974.517 kb on - strandat 2974.521 kb on + strandat 2974.529 kb on - strandat 2974.550 kb on - strandat 2974.561 kb on + strandat 2974.642 kb on + strandat 2974.678 kb on + strandat 2974.730 kb on - strandat 2974.732 kb on + strandat 2974.740 kb on - strandat 2974.770 kb on + strand, within BWI76_RS14210at 2974.774 kb on - strand, within BWI76_RS14210at 2974.774 kb on - strand, within BWI76_RS14210at 2974.778 kb on - strand, within BWI76_RS14210at 2974.778 kb on - strand, within BWI76_RS14210at 2974.792 kb on + strand, within BWI76_RS14210at 2974.801 kb on - strand, within BWI76_RS14210at 2974.801 kb on - strand, within BWI76_RS14210at 2974.902 kb on + strand, within BWI76_RS14210at 2974.902 kb on + strand, within BWI76_RS14210at 2974.904 kb on - strand, within BWI76_RS14210at 2974.957 kb on - strand, within BWI76_RS14210at 2974.998 kb on + strand, within BWI76_RS14210at 2975.194 kb on + strand, within BWI76_RS14210at 2975.275 kb on - strand, within BWI76_RS14210

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1
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2,972,572 - BWI76_RS14200 0.58 +1.1
2,972,596 + BWI76_RS14200 0.60 -1.6
2,972,635 - BWI76_RS14200 0.63 -2.0
2,973,711 + BWI76_RS14205 0.44 -1.4
2,973,745 + BWI76_RS14205 0.46 -1.9
2,973,745 + BWI76_RS14205 0.46 +0.3
2,973,745 + BWI76_RS14205 0.46 +2.1
2,973,745 + BWI76_RS14205 0.46 -1.8
2,973,753 - BWI76_RS14205 0.47 -2.8
2,973,753 - BWI76_RS14205 0.47 +0.3
2,973,753 - BWI76_RS14205 0.47 -0.7
2,973,753 - BWI76_RS14205 0.47 -0.9
2,973,866 + BWI76_RS14205 0.55 -2.0
2,973,997 - BWI76_RS14205 0.65 +1.1
2,974,002 - BWI76_RS14205 0.65 +0.5
2,974,027 + BWI76_RS14205 0.67 -0.1
2,974,056 + BWI76_RS14205 0.69 -1.7
2,974,056 + BWI76_RS14205 0.69 +0.8
2,974,197 - BWI76_RS14205 0.79 -1.2
2,974,284 - BWI76_RS14205 0.86 -3.5
2,974,349 + +1.1
2,974,349 + -0.8
2,974,349 + +0.8
2,974,349 + -1.2
2,974,349 + -1.1
2,974,357 - -1.7
2,974,357 - -0.7
2,974,367 - +0.9
2,974,371 + +0.7
2,974,402 + -0.1
2,974,405 + -1.8
2,974,413 - +1.7
2,974,442 + +0.2
2,974,450 + -2.9
2,974,476 + -1.0
2,974,517 - -2.6
2,974,521 + -2.0
2,974,529 - -0.8
2,974,550 - -1.8
2,974,561 + -2.2
2,974,642 + -1.3
2,974,678 + -0.1
2,974,730 - -2.4
2,974,732 + -3.2
2,974,740 - -0.6
2,974,770 + BWI76_RS14210 0.11 -0.9
2,974,774 - BWI76_RS14210 0.12 -0.3
2,974,774 - BWI76_RS14210 0.12 -0.4
2,974,778 - BWI76_RS14210 0.12 -2.6
2,974,778 - BWI76_RS14210 0.12 -0.2
2,974,792 + BWI76_RS14210 0.13 +0.8
2,974,801 - BWI76_RS14210 0.14 -0.1
2,974,801 - BWI76_RS14210 0.14 -2.9
2,974,902 + BWI76_RS14210 0.25 +0.5
2,974,902 + BWI76_RS14210 0.25 -1.1
2,974,904 - BWI76_RS14210 0.25 -1.4
2,974,957 - BWI76_RS14210 0.31 +0.2
2,974,998 + BWI76_RS14210 0.35 +0.3
2,975,194 + BWI76_RS14210 0.55 +1.0
2,975,275 - BWI76_RS14210 0.64 +0.9

Or see this region's nucleotide sequence