Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS13950

Experiment: KLEB20 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS13940 and BWI76_RS13945 are separated by 153 nucleotidesBWI76_RS13945 and BWI76_RS13950 are separated by 46 nucleotidesBWI76_RS13950 and BWI76_RS13955 are separated by 43 nucleotidesBWI76_RS13955 and BWI76_RS13960 are separated by 346 nucleotides BWI76_RS13940: BWI76_RS13940 - membrane protein, at 2,911,090 to 2,911,554 _RS13940 BWI76_RS13945: BWI76_RS13945 - lysine exporter protein LysE/YggA, at 2,911,708 to 2,912,340 _RS13945 BWI76_RS13950: BWI76_RS13950 - hypothetical protein, at 2,912,387 to 2,913,079 _RS13950 BWI76_RS13955: BWI76_RS13955 - hypothetical protein, at 2,913,123 to 2,913,725 _RS13955 BWI76_RS13960: BWI76_RS13960 - urea ABC transporter periplasmic protein, at 2,914,072 to 2,915,130 _RS13960 Position (kb) 2912 2913 2914Strain fitness (log2 ratio) -2 -1 0 1at 2911.865 kb on + strand, within BWI76_RS13945at 2912.087 kb on - strand, within BWI76_RS13945at 2912.092 kb on + strand, within BWI76_RS13945at 2912.356 kb on + strandat 2912.671 kb on + strand, within BWI76_RS13950at 2912.740 kb on + strand, within BWI76_RS13950at 2912.823 kb on - strand, within BWI76_RS13950at 2912.962 kb on + strand, within BWI76_RS13950at 2912.980 kb on - strand, within BWI76_RS13950at 2913.054 kb on - strandat 2913.300 kb on + strand, within BWI76_RS13955at 2913.662 kb on - strand, within BWI76_RS13955at 2913.747 kb on + strandat 2913.947 kb on + strandat 2913.953 kb on + strandat 2913.993 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1
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2,911,865 + BWI76_RS13945 0.25 +0.3
2,912,087 - BWI76_RS13945 0.60 +0.1
2,912,092 + BWI76_RS13945 0.61 -1.1
2,912,356 + +0.3
2,912,671 + BWI76_RS13950 0.41 -0.6
2,912,740 + BWI76_RS13950 0.51 -1.3
2,912,823 - BWI76_RS13950 0.63 -2.5
2,912,962 + BWI76_RS13950 0.83 -1.7
2,912,980 - BWI76_RS13950 0.86 +0.2
2,913,054 - -0.1
2,913,300 + BWI76_RS13955 0.29 -1.4
2,913,662 - BWI76_RS13955 0.89 +0.3
2,913,747 + -0.4
2,913,947 + +0.2
2,913,953 + -0.5
2,913,993 - -0.3

Or see this region's nucleotide sequence