Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10275 and BWI76_RS10280 overlap by 4 nucleotides BWI76_RS10280 and BWI76_RS10285 are separated by 28 nucleotides BWI76_RS10285 and BWI76_RS10290 are separated by 191 nucleotides
BWI76_RS10275: BWI76_RS10275 - ABC transporter, at 2,176,334 to 2,177,074
_RS10275
BWI76_RS10280: BWI76_RS10280 - polar amino acid ABC transporter permease, at 2,177,071 to 2,177,796
_RS10280
BWI76_RS10285: BWI76_RS10285 - family 3 extracellular solute-binding protein, at 2,177,825 to 2,178,637
_RS10285
BWI76_RS10290: BWI76_RS10290 - transcriptional regulator, at 2,178,829 to 2,179,725
_RS10290
Position (kb)
2177
2178
2179 Strain fitness (log2 ratio)
-1
0
1 at 2176.990 kb on + strand, within BWI76_RS10275 at 2177.183 kb on + strand, within BWI76_RS10280 at 2177.191 kb on - strand, within BWI76_RS10280 at 2177.663 kb on + strand, within BWI76_RS10280 at 2177.696 kb on + strand, within BWI76_RS10280 at 2177.701 kb on + strand, within BWI76_RS10280 at 2177.706 kb on + strand, within BWI76_RS10280 at 2177.793 kb on + strand at 2177.981 kb on - strand, within BWI76_RS10285 at 2178.350 kb on - strand, within BWI76_RS10285 at 2178.471 kb on + strand, within BWI76_RS10285 at 2178.488 kb on - strand, within BWI76_RS10285 at 2178.842 kb on - strand at 2179.198 kb on + strand, within BWI76_RS10290 at 2179.198 kb on + strand, within BWI76_RS10290 at 2179.206 kb on - strand, within BWI76_RS10290 at 2179.206 kb on - strand, within BWI76_RS10290 at 2179.255 kb on + strand, within BWI76_RS10290 at 2179.499 kb on + strand, within BWI76_RS10290 at 2179.507 kb on - strand at 2179.520 kb on + strand at 2179.622 kb on + strand, within BWI76_RS10290
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 2,176,990 + BWI76_RS10275 0.89 +0.1 2,177,183 + BWI76_RS10280 0.15 -0.6 2,177,191 - BWI76_RS10280 0.17 +1.7 2,177,663 + BWI76_RS10280 0.82 +0.5 2,177,696 + BWI76_RS10280 0.86 +0.7 2,177,701 + BWI76_RS10280 0.87 +0.5 2,177,706 + BWI76_RS10280 0.87 +0.9 2,177,793 + -1.6 2,177,981 - BWI76_RS10285 0.19 -1.1 2,178,350 - BWI76_RS10285 0.65 -0.2 2,178,471 + BWI76_RS10285 0.79 -0.2 2,178,488 - BWI76_RS10285 0.82 -0.5 2,178,842 - -0.1 2,179,198 + BWI76_RS10290 0.41 -0.1 2,179,198 + BWI76_RS10290 0.41 -1.5 2,179,206 - BWI76_RS10290 0.42 -0.3 2,179,206 - BWI76_RS10290 0.42 -0.5 2,179,255 + BWI76_RS10290 0.47 -1.5 2,179,499 + BWI76_RS10290 0.75 -0.1 2,179,507 - +1.7 2,179,520 + +1.7 2,179,622 + BWI76_RS10290 0.88 -0.6
Or see this region's nucleotide sequence