Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10035 and BWI76_RS10040 are separated by 36 nucleotides BWI76_RS10040 and BWI76_RS10045 are separated by 60 nucleotides BWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotides BWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotides
BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908
_RS10035
BWI76_RS10040: BWI76_RS10040 - heat-shock protein HspQ, at 2,127,945 to 2,128,262
_RS10040
BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525
_RS10045
BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270
_RS10050
BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074
_RS10055
Position (kb)
2128
2129
2130 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2127.326 kb on + strand, within BWI76_RS10035 at 2127.835 kb on + strand at 2128.217 kb on - strand, within BWI76_RS10040 at 2128.495 kb on - strand, within BWI76_RS10045 at 2128.714 kb on - strand, within BWI76_RS10045 at 2128.762 kb on - strand, within BWI76_RS10045 at 2128.900 kb on - strand, within BWI76_RS10045 at 2129.803 kb on + strand, within BWI76_RS10050 at 2129.811 kb on - strand, within BWI76_RS10050 at 2129.980 kb on + strand, within BWI76_RS10050 at 2130.069 kb on - strand, within BWI76_RS10050 at 2130.140 kb on + strand, within BWI76_RS10050 at 2130.164 kb on + strand, within BWI76_RS10050 at 2130.246 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 2,127,326 + BWI76_RS10035 0.38 +0.4 2,127,835 + +0.1 2,128,217 - BWI76_RS10040 0.86 +1.3 2,128,495 - BWI76_RS10045 0.14 -1.2 2,128,714 - BWI76_RS10045 0.33 -0.4 2,128,762 - BWI76_RS10045 0.36 -1.4 2,128,900 - BWI76_RS10045 0.48 -3.2 2,129,803 + BWI76_RS10050 0.16 -0.2 2,129,811 - BWI76_RS10050 0.17 +0.8 2,129,980 + BWI76_RS10050 0.48 +1.6 2,130,069 - BWI76_RS10050 0.64 +0.3 2,130,140 + BWI76_RS10050 0.76 -0.1 2,130,164 + BWI76_RS10050 0.81 -0.6 2,130,246 + +0.8
Or see this region's nucleotide sequence