Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10045

Experiment: KLEB20 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10035 and BWI76_RS10040 are separated by 36 nucleotidesBWI76_RS10040 and BWI76_RS10045 are separated by 60 nucleotidesBWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotidesBWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotides BWI76_RS10035: BWI76_RS10035 - hypothetical protein, at 2,126,976 to 2,127,908 _RS10035 BWI76_RS10040: BWI76_RS10040 - heat-shock protein HspQ, at 2,127,945 to 2,128,262 _RS10040 BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525 _RS10045 BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270 _RS10050 BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074 _RS10055 Position (kb) 2128 2129 2130Strain fitness (log2 ratio) -3 -2 -1 0 1at 2127.326 kb on + strand, within BWI76_RS10035at 2127.835 kb on + strandat 2128.217 kb on - strand, within BWI76_RS10040at 2128.495 kb on - strand, within BWI76_RS10045at 2128.714 kb on - strand, within BWI76_RS10045at 2128.762 kb on - strand, within BWI76_RS10045at 2128.900 kb on - strand, within BWI76_RS10045at 2129.803 kb on + strand, within BWI76_RS10050at 2129.811 kb on - strand, within BWI76_RS10050at 2129.980 kb on + strand, within BWI76_RS10050at 2130.069 kb on - strand, within BWI76_RS10050at 2130.140 kb on + strand, within BWI76_RS10050at 2130.164 kb on + strand, within BWI76_RS10050at 2130.246 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1
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2,127,326 + BWI76_RS10035 0.38 +0.4
2,127,835 + +0.1
2,128,217 - BWI76_RS10040 0.86 +1.3
2,128,495 - BWI76_RS10045 0.14 -1.2
2,128,714 - BWI76_RS10045 0.33 -0.4
2,128,762 - BWI76_RS10045 0.36 -1.4
2,128,900 - BWI76_RS10045 0.48 -3.2
2,129,803 + BWI76_RS10050 0.16 -0.2
2,129,811 - BWI76_RS10050 0.17 +0.8
2,129,980 + BWI76_RS10050 0.48 +1.6
2,130,069 - BWI76_RS10050 0.64 +0.3
2,130,140 + BWI76_RS10050 0.76 -0.1
2,130,164 + BWI76_RS10050 0.81 -0.6
2,130,246 + +0.8

Or see this region's nucleotide sequence