Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09450 and BWI76_RS09455 are separated by 89 nucleotides BWI76_RS09455 and BWI76_RS09460 are separated by 349 nucleotides BWI76_RS09460 and BWI76_RS09465 are separated by 57 nucleotides
BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657
_RS09450
BWI76_RS09455: BWI76_RS09455 - YbjN domain-containing protein, at 1,979,747 to 1,980,226
_RS09455
BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688
_RS09460
BWI76_RS09465: BWI76_RS09465 - polyamine ABC transporter ATP-binding protein, at 1,981,746 to 1,982,924
_RS09465
Position (kb)
1980
1981
1982 Strain fitness (log2 ratio)
-2
-1
0
1 at 1979.932 kb on + strand, within BWI76_RS09455 at 1980.089 kb on - strand, within BWI76_RS09455 at 1980.110 kb on - strand, within BWI76_RS09455 at 1980.153 kb on - strand, within BWI76_RS09455 at 1980.174 kb on - strand, within BWI76_RS09455 at 1980.403 kb on - strand at 1980.419 kb on + strand at 1980.606 kb on + strand at 1980.611 kb on + strand at 1980.611 kb on + strand at 1980.619 kb on - strand at 1980.619 kb on - strand at 1980.675 kb on - strand at 1980.790 kb on - strand, within BWI76_RS09460 at 1980.816 kb on - strand, within BWI76_RS09460 at 1980.864 kb on - strand, within BWI76_RS09460 at 1981.028 kb on + strand, within BWI76_RS09460 at 1981.036 kb on - strand, within BWI76_RS09460 at 1981.504 kb on - strand, within BWI76_RS09460 at 1982.027 kb on - strand, within BWI76_RS09465 at 1982.245 kb on - strand, within BWI76_RS09465 at 1982.359 kb on - strand, within BWI76_RS09465 at 1982.535 kb on - strand, within BWI76_RS09465 at 1982.595 kb on + strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.603 kb on - strand, within BWI76_RS09465 at 1982.613 kb on - strand, within BWI76_RS09465
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 1,979,932 + BWI76_RS09455 0.39 +0.5 1,980,089 - BWI76_RS09455 0.71 +1.4 1,980,110 - BWI76_RS09455 0.76 +1.5 1,980,153 - BWI76_RS09455 0.85 -1.1 1,980,174 - BWI76_RS09455 0.89 -0.7 1,980,403 - +0.5 1,980,419 + +1.3 1,980,606 + -0.5 1,980,611 + +0.9 1,980,611 + +0.6 1,980,619 - +0.4 1,980,619 - +0.1 1,980,675 - +0.8 1,980,790 - BWI76_RS09460 0.19 +1.3 1,980,816 - BWI76_RS09460 0.22 -2.3 1,980,864 - BWI76_RS09460 0.26 -1.6 1,981,028 + BWI76_RS09460 0.41 -0.7 1,981,036 - BWI76_RS09460 0.41 -0.6 1,981,504 - BWI76_RS09460 0.83 -1.3 1,982,027 - BWI76_RS09465 0.24 +0.4 1,982,245 - BWI76_RS09465 0.42 +1.4 1,982,359 - BWI76_RS09465 0.52 -0.2 1,982,535 - BWI76_RS09465 0.67 -0.1 1,982,595 + BWI76_RS09465 0.72 -2.5 1,982,603 - BWI76_RS09465 0.73 -2.1 1,982,603 - BWI76_RS09465 0.73 -0.8 1,982,613 - BWI76_RS09465 0.74 -1.4
Or see this region's nucleotide sequence