Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09100 and BWI76_RS09105 are separated by 52 nucleotides BWI76_RS09105 and BWI76_RS09110 are separated by 1 nucleotides BWI76_RS09110 and BWI76_RS09115 are separated by 182 nucleotides
BWI76_RS09100: BWI76_RS09100 - glutathione ABC transporter substrate-binding protein GsiB, at 1,922,108 to 1,923,646
_RS09100
BWI76_RS09105: BWI76_RS09105 - glutathione ABC transporter permease GsiC, at 1,923,699 to 1,924,619
_RS09105
BWI76_RS09110: BWI76_RS09110 - glutathione ABC transporter permease GsiD, at 1,924,621 to 1,925,532
_RS09110
BWI76_RS09115: BWI76_RS09115 - 30S ribosomal protein S12 methylthiotransferase RimO, at 1,925,715 to 1,927,040
_RS09115
Position (kb)
1924
1925
1926 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1923.992 kb on + strand, within BWI76_RS09105 at 1924.096 kb on - strand, within BWI76_RS09105 at 1924.452 kb on + strand, within BWI76_RS09105 at 1924.671 kb on + strand at 1924.681 kb on + strand at 1924.754 kb on + strand, within BWI76_RS09110 at 1924.779 kb on + strand, within BWI76_RS09110 at 1924.996 kb on + strand, within BWI76_RS09110 at 1925.639 kb on + strand at 1925.647 kb on - strand at 1925.647 kb on - strand at 1925.654 kb on + strand at 1926.086 kb on - strand, within BWI76_RS09115 at 1926.161 kb on + strand at 1926.194 kb on + strand, within BWI76_RS09115 at 1926.377 kb on + strand, within BWI76_RS09115
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 1,923,992 + BWI76_RS09105 0.32 -0.0 1,924,096 - BWI76_RS09105 0.43 -2.7 1,924,452 + BWI76_RS09105 0.82 +0.4 1,924,671 + -0.3 1,924,681 + -1.2 1,924,754 + BWI76_RS09110 0.15 +1.3 1,924,779 + BWI76_RS09110 0.17 -1.8 1,924,996 + BWI76_RS09110 0.41 -0.9 1,925,639 + +2.5 1,925,647 - -0.5 1,925,647 - -0.1 1,925,654 + -0.0 1,926,086 - BWI76_RS09115 0.28 -0.6 1,926,161 + +1.7 1,926,194 + BWI76_RS09115 0.36 +1.1 1,926,377 + BWI76_RS09115 0.50 +0.7
Or see this region's nucleotide sequence