Experiment: KLEB20 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04085 and BWI76_RS04090 overlap by 32 nucleotides BWI76_RS04090 and BWI76_RS04095 are separated by 14 nucleotides BWI76_RS04095 and BWI76_RS04100 are separated by 91 nucleotides
BWI76_RS04085: BWI76_RS04085 - DNA polymerase III subunit psi, at 841,445 to 841,858
_RS04085
BWI76_RS04090: BWI76_RS04090 - ribosomal-protein-alanine N-acetyltransferase RimI, at 841,827 to 842,273
_RS04090
BWI76_RS04095: BWI76_RS04095 - dUMP phosphatase, at 842,288 to 842,965
_RS04095
BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646
_RS04100
Position (kb) 842
843 Strain fitness (log2 ratio) -2
-1
0
1 at 841.857 kb on + strand at 841.983 kb on + strand, within BWI76_RS04090 at 842.066 kb on + strand, within BWI76_RS04090 at 842.076 kb on - strand, within BWI76_RS04090 at 842.128 kb on + strand, within BWI76_RS04090 at 842.143 kb on + strand, within BWI76_RS04090 at 842.730 kb on - strand, within BWI76_RS04095 at 842.876 kb on - strand, within BWI76_RS04095 at 843.799 kb on - strand, within BWI76_RS04100 at 843.868 kb on + strand, within BWI76_RS04100
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 0.1 remove 841,857 + +0.4 841,983 + BWI76_RS04090 0.35 -0.2 842,066 + BWI76_RS04090 0.53 -1.5 842,076 - BWI76_RS04090 0.56 -1.7 842,128 + BWI76_RS04090 0.67 -0.0 842,143 + BWI76_RS04090 0.71 +0.3 842,730 - BWI76_RS04095 0.65 -0.2 842,876 - BWI76_RS04095 0.87 -2.4 843,799 - BWI76_RS04100 0.47 -2.5 843,868 + BWI76_RS04100 0.51 -2.5
Or see this region's nucleotide sequence