Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27265 and BWI76_RS27270 are separated by 3 nucleotides BWI76_RS27270 and BWI76_RS27275 overlap by 1 nucleotides BWI76_RS27275 and BWI76_RS27280 overlap by 23 nucleotides
BWI76_RS27265: BWI76_RS27265 - ADP-heptose--LPS heptosyltransferase II, at 5,658,863 to 5,659,921
_RS27265
BWI76_RS27270: BWI76_RS27270 - ADP-heptose--LPS heptosyl transferase I, at 5,659,925 to 5,660,896
_RS27270
BWI76_RS27275: BWI76_RS27275 - hypothetical protein, at 5,660,896 to 5,661,804
_RS27275
BWI76_RS27280: BWI76_RS27280 - polymerase, at 5,661,782 to 5,662,948
_RS27280
Position (kb) 5659
5660
5661 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 5660.898 kb on - strand at 5660.979 kb on + strand at 5661.090 kb on - strand, within BWI76_RS27275 at 5661.167 kb on - strand, within BWI76_RS27275 at 5661.293 kb on - strand, within BWI76_RS27275 at 5661.334 kb on + strand, within BWI76_RS27275 at 5661.444 kb on - strand, within BWI76_RS27275 at 5661.527 kb on - strand, within BWI76_RS27275 at 5661.527 kb on - strand, within BWI76_RS27275 at 5661.527 kb on - strand, within BWI76_RS27275 at 5661.560 kb on - strand, within BWI76_RS27275 at 5661.570 kb on - strand, within BWI76_RS27275 at 5661.619 kb on + strand, within BWI76_RS27275 at 5661.689 kb on + strand, within BWI76_RS27275 at 5661.820 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 5,660,898 - +7.2 5,660,979 + +7.9 5,661,090 - BWI76_RS27275 0.21 +8.1 5,661,167 - BWI76_RS27275 0.30 +8.0 5,661,293 - BWI76_RS27275 0.44 +7.6 5,661,334 + BWI76_RS27275 0.48 +7.2 5,661,444 - BWI76_RS27275 0.60 +8.4 5,661,527 - BWI76_RS27275 0.69 +7.2 5,661,527 - BWI76_RS27275 0.69 +8.1 5,661,527 - BWI76_RS27275 0.69 +7.6 5,661,560 - BWI76_RS27275 0.73 +7.9 5,661,570 - BWI76_RS27275 0.74 +8.1 5,661,619 + BWI76_RS27275 0.80 +7.9 5,661,689 + BWI76_RS27275 0.87 +7.5 5,661,820 - -2.9
Or see this region's nucleotide sequence