Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25235 and BWI76_RS25240 are separated by 14 nucleotides BWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotides BWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides
BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399
_RS25235
BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400
_RS25240
BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981
_RS25245
BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553
_RS25250
Position (kb) 5248
5249
5250 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5247.432 kb on - strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.553 kb on + strand, within BWI76_RS25235 at 5247.605 kb on - strand, within BWI76_RS25235 at 5247.633 kb on + strand, within BWI76_RS25235 at 5247.699 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand at 5247.998 kb on + strand, within BWI76_RS25235 at 5248.166 kb on + strand, within BWI76_RS25235 at 5248.283 kb on + strand, within BWI76_RS25235 at 5248.369 kb on + strand at 5248.369 kb on + strand at 5248.377 kb on - strand at 5248.409 kb on + strand at 5248.489 kb on + strand at 5248.489 kb on + strand at 5248.694 kb on - strand, within BWI76_RS25240 at 5248.730 kb on - strand, within BWI76_RS25240 at 5248.858 kb on - strand, within BWI76_RS25240 at 5248.882 kb on + strand, within BWI76_RS25240 at 5248.907 kb on - strand, within BWI76_RS25240 at 5248.917 kb on + strand, within BWI76_RS25240 at 5249.215 kb on - strand, within BWI76_RS25240 at 5249.395 kb on + strand at 5249.446 kb on + strand at 5249.595 kb on + strand, within BWI76_RS25245 at 5249.720 kb on + strand, within BWI76_RS25245 at 5249.823 kb on + strand, within BWI76_RS25245 at 5249.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 5,247,432 - -1.1 5,247,487 + -0.5 5,247,487 + -1.7 5,247,487 + -0.2 5,247,487 + -0.4 5,247,553 + BWI76_RS25235 0.13 +1.8 5,247,605 - BWI76_RS25235 0.19 -1.6 5,247,633 + BWI76_RS25235 0.22 -2.9 5,247,699 - BWI76_RS25235 0.28 -4.3 5,247,883 - BWI76_RS25235 0.47 +1.6 5,247,883 - -0.7 5,247,998 + BWI76_RS25235 0.59 -1.1 5,248,166 + BWI76_RS25235 0.76 -0.2 5,248,283 + BWI76_RS25235 0.88 -0.1 5,248,369 + +2.1 5,248,369 + +1.8 5,248,377 - +1.5 5,248,409 + -0.6 5,248,489 + -0.6 5,248,489 + +0.4 5,248,694 - BWI76_RS25240 0.28 +1.4 5,248,730 - BWI76_RS25240 0.32 +1.0 5,248,858 - BWI76_RS25240 0.45 +3.6 5,248,882 + BWI76_RS25240 0.47 -1.2 5,248,907 - BWI76_RS25240 0.50 -2.4 5,248,917 + BWI76_RS25240 0.51 -0.2 5,249,215 - BWI76_RS25240 0.81 +4.0 5,249,395 + -1.4 5,249,446 + +1.3 5,249,595 + BWI76_RS25245 0.32 +0.8 5,249,720 + BWI76_RS25245 0.54 +0.4 5,249,823 + BWI76_RS25245 0.72 -2.9 5,249,945 + -0.2
Or see this region's nucleotide sequence