Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23620 and BWI76_RS23625 overlap by 4 nucleotides BWI76_RS23625 and BWI76_RS23630 are separated by 166 nucleotides BWI76_RS23630 and BWI76_RS23635 are separated by 40 nucleotides
BWI76_RS23620: BWI76_RS23620 - multidrug resistance transporter, at 4,925,232 to 4,926,710
_RS23620
BWI76_RS23625: BWI76_RS23625 - helix-turn-helix transcriptional regulator, at 4,926,707 to 4,929,418
_RS23625
BWI76_RS23630: BWI76_RS23630 - acetoin(diacetyl) reductase, at 4,929,585 to 4,930,364
_RS23630
BWI76_RS23635: BWI76_RS23635 - MFS transporter, at 4,930,405 to 4,931,958
_RS23635
Position (kb) 4926
4927
4928
4929
4930 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4925.802 kb on - strand, within BWI76_RS23620 at 4925.833 kb on - strand, within BWI76_RS23620 at 4925.894 kb on - strand, within BWI76_RS23620 at 4925.900 kb on + strand, within BWI76_RS23620 at 4925.901 kb on - strand, within BWI76_RS23620 at 4926.115 kb on + strand, within BWI76_RS23620 at 4926.115 kb on + strand, within BWI76_RS23620 at 4926.285 kb on - strand, within BWI76_RS23620 at 4926.392 kb on + strand, within BWI76_RS23620 at 4926.637 kb on + strand at 4926.694 kb on - strand at 4926.841 kb on + strand at 4927.036 kb on + strand, within BWI76_RS23625 at 4927.176 kb on + strand, within BWI76_RS23625 at 4927.191 kb on - strand, within BWI76_RS23625 at 4927.229 kb on - strand, within BWI76_RS23625 at 4927.540 kb on + strand, within BWI76_RS23625 at 4927.614 kb on - strand, within BWI76_RS23625 at 4927.647 kb on - strand, within BWI76_RS23625 at 4927.688 kb on - strand, within BWI76_RS23625 at 4927.722 kb on + strand, within BWI76_RS23625 at 4927.742 kb on + strand at 4928.010 kb on - strand, within BWI76_RS23625 at 4928.291 kb on - strand, within BWI76_RS23625 at 4928.469 kb on + strand, within BWI76_RS23625 at 4928.514 kb on - strand, within BWI76_RS23625 at 4928.514 kb on - strand, within BWI76_RS23625 at 4928.514 kb on - strand, within BWI76_RS23625 at 4928.542 kb on + strand, within BWI76_RS23625 at 4928.587 kb on + strand, within BWI76_RS23625 at 4928.777 kb on + strand, within BWI76_RS23625 at 4928.786 kb on + strand, within BWI76_RS23625 at 4928.833 kb on - strand, within BWI76_RS23625 at 4928.838 kb on - strand, within BWI76_RS23625 at 4928.839 kb on - strand, within BWI76_RS23625 at 4929.058 kb on + strand, within BWI76_RS23625 at 4929.166 kb on + strand at 4929.235 kb on + strand at 4929.356 kb on + strand at 4929.431 kb on + strand at 4929.439 kb on - strand at 4929.574 kb on - strand at 4929.624 kb on + strand at 4929.788 kb on + strand, within BWI76_RS23630 at 4929.788 kb on + strand, within BWI76_RS23630 at 4929.788 kb on + strand, within BWI76_RS23630 at 4929.829 kb on + strand, within BWI76_RS23630 at 4930.286 kb on - strand, within BWI76_RS23630
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 4,925,802 - BWI76_RS23620 0.39 +1.2 4,925,833 - BWI76_RS23620 0.41 +1.3 4,925,894 - BWI76_RS23620 0.45 -0.1 4,925,900 + BWI76_RS23620 0.45 +0.5 4,925,901 - BWI76_RS23620 0.45 -0.8 4,926,115 + BWI76_RS23620 0.60 -3.0 4,926,115 + BWI76_RS23620 0.60 +0.6 4,926,285 - BWI76_RS23620 0.71 +0.6 4,926,392 + BWI76_RS23620 0.78 -0.1 4,926,637 + -1.2 4,926,694 - -0.4 4,926,841 + +0.6 4,927,036 + BWI76_RS23625 0.12 -3.0 4,927,176 + BWI76_RS23625 0.17 -0.1 4,927,191 - BWI76_RS23625 0.18 -0.3 4,927,229 - BWI76_RS23625 0.19 -1.5 4,927,540 + BWI76_RS23625 0.31 -0.5 4,927,614 - BWI76_RS23625 0.33 +0.8 4,927,647 - BWI76_RS23625 0.35 -0.6 4,927,688 - BWI76_RS23625 0.36 +0.8 4,927,722 + BWI76_RS23625 0.37 -0.7 4,927,742 + -1.7 4,928,010 - BWI76_RS23625 0.48 +0.5 4,928,291 - BWI76_RS23625 0.58 -3.0 4,928,469 + BWI76_RS23625 0.65 +0.9 4,928,514 - BWI76_RS23625 0.67 -0.2 4,928,514 - BWI76_RS23625 0.67 +0.8 4,928,514 - BWI76_RS23625 0.67 -2.2 4,928,542 + BWI76_RS23625 0.68 -0.2 4,928,587 + BWI76_RS23625 0.69 -1.8 4,928,777 + BWI76_RS23625 0.76 -0.5 4,928,786 + BWI76_RS23625 0.77 -2.0 4,928,833 - BWI76_RS23625 0.78 -0.2 4,928,838 - BWI76_RS23625 0.79 +0.2 4,928,839 - BWI76_RS23625 0.79 -1.3 4,929,058 + BWI76_RS23625 0.87 -0.6 4,929,166 + +0.4 4,929,235 + -3.0 4,929,356 + +0.2 4,929,431 + +2.2 4,929,439 - +0.1 4,929,574 - -0.5 4,929,624 + -0.2 4,929,788 + BWI76_RS23630 0.26 -0.2 4,929,788 + BWI76_RS23630 0.26 +0.7 4,929,788 + BWI76_RS23630 0.26 +0.5 4,929,829 + BWI76_RS23630 0.31 +0.5 4,930,286 - BWI76_RS23630 0.90 +0.3
Or see this region's nucleotide sequence