Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23370 and BWI76_RS23375 are separated by 135 nucleotides BWI76_RS23375 and BWI76_RS23380 are separated by 493 nucleotides BWI76_RS23380 and BWI76_RS23385 overlap by 8 nucleotides BWI76_RS23385 and BWI76_RS23390 are separated by 12 nucleotides
BWI76_RS23370: BWI76_RS23370 - aspartate/glutamate racemase, at 4,873,650 to 4,874,342
_RS23370
BWI76_RS23375: BWI76_RS23375 - cupin, at 4,874,478 to 4,874,804
_RS23375
BWI76_RS23380: BWI76_RS23380 - sugar ABC transporter permease, at 4,875,298 to 4,876,188
_RS23380
BWI76_RS23385: BWI76_RS23385 - sugar ABC transporter permease, at 4,876,181 to 4,877,083
_RS23385
BWI76_RS23390: BWI76_RS23390 - oligogalacturonide ABC transporter ATP-binding protein, at 4,877,096 to 4,878,223
_RS23390
Position (kb) 4875
4876
4877 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4874.311 kb on - strand at 4874.519 kb on - strand, within BWI76_RS23375 at 4874.519 kb on - strand, within BWI76_RS23375 at 4874.578 kb on + strand, within BWI76_RS23375 at 4874.627 kb on + strand, within BWI76_RS23375 at 4874.627 kb on + strand, within BWI76_RS23375 at 4874.632 kb on + strand, within BWI76_RS23375 at 4874.632 kb on + strand, within BWI76_RS23375 at 4874.640 kb on - strand, within BWI76_RS23375 at 4874.821 kb on - strand at 4874.821 kb on - strand at 4874.941 kb on + strand at 4874.950 kb on - strand at 4874.961 kb on + strand at 4874.961 kb on + strand at 4874.961 kb on + strand at 4874.969 kb on - strand at 4874.969 kb on - strand at 4874.969 kb on - strand at 4875.256 kb on + strand at 4875.287 kb on + strand at 4875.404 kb on - strand, within BWI76_RS23380 at 4875.430 kb on - strand, within BWI76_RS23380 at 4875.430 kb on - strand, within BWI76_RS23380 at 4875.457 kb on - strand, within BWI76_RS23380 at 4875.497 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.580 kb on - strand, within BWI76_RS23380 at 4875.702 kb on - strand, within BWI76_RS23380 at 4875.721 kb on - strand, within BWI76_RS23380 at 4875.739 kb on - strand, within BWI76_RS23380 at 4875.769 kb on - strand, within BWI76_RS23380 at 4876.020 kb on + strand, within BWI76_RS23380 at 4876.047 kb on - strand at 4876.086 kb on - strand, within BWI76_RS23380 at 4876.086 kb on - strand, within BWI76_RS23380 at 4876.233 kb on - strand at 4876.310 kb on - strand, within BWI76_RS23385 at 4876.322 kb on + strand, within BWI76_RS23385 at 4876.371 kb on + strand, within BWI76_RS23385 at 4876.444 kb on - strand, within BWI76_RS23385 at 4876.610 kb on - strand, within BWI76_RS23385 at 4876.613 kb on - strand, within BWI76_RS23385 at 4876.694 kb on + strand, within BWI76_RS23385 at 4876.798 kb on + strand, within BWI76_RS23385 at 4877.043 kb on + strand at 4877.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 4,874,311 - +0.5 4,874,519 - BWI76_RS23375 0.13 +0.6 4,874,519 - BWI76_RS23375 0.13 +1.5 4,874,578 + BWI76_RS23375 0.31 -0.9 4,874,627 + BWI76_RS23375 0.46 -0.6 4,874,627 + BWI76_RS23375 0.46 +0.4 4,874,632 + BWI76_RS23375 0.47 +2.2 4,874,632 + BWI76_RS23375 0.47 +0.7 4,874,640 - BWI76_RS23375 0.50 -2.1 4,874,821 - -0.1 4,874,821 - -0.1 4,874,941 + +0.2 4,874,950 - -0.0 4,874,961 + +0.3 4,874,961 + -0.4 4,874,961 + +0.1 4,874,969 - -1.4 4,874,969 - +0.7 4,874,969 - -0.2 4,875,256 + +0.5 4,875,287 + -0.4 4,875,404 - BWI76_RS23380 0.12 -0.5 4,875,430 - BWI76_RS23380 0.15 -1.2 4,875,430 - BWI76_RS23380 0.15 +0.6 4,875,457 - BWI76_RS23380 0.18 -1.9 4,875,497 - BWI76_RS23380 0.22 -0.5 4,875,580 - BWI76_RS23380 0.32 -0.3 4,875,580 - BWI76_RS23380 0.32 -0.6 4,875,580 - BWI76_RS23380 0.32 -1.7 4,875,580 - BWI76_RS23380 0.32 -0.1 4,875,580 - BWI76_RS23380 0.32 -0.7 4,875,580 - BWI76_RS23380 0.32 +0.3 4,875,580 - BWI76_RS23380 0.32 +0.2 4,875,702 - BWI76_RS23380 0.45 -2.1 4,875,721 - BWI76_RS23380 0.47 +0.3 4,875,739 - BWI76_RS23380 0.49 +0.4 4,875,769 - BWI76_RS23380 0.53 -2.6 4,876,020 + BWI76_RS23380 0.81 -0.3 4,876,047 - +2.0 4,876,086 - BWI76_RS23380 0.88 -1.8 4,876,086 - BWI76_RS23380 0.88 -0.2 4,876,233 - +0.4 4,876,310 - BWI76_RS23385 0.14 -0.1 4,876,322 + BWI76_RS23385 0.16 -0.4 4,876,371 + BWI76_RS23385 0.21 -2.9 4,876,444 - BWI76_RS23385 0.29 +0.5 4,876,610 - BWI76_RS23385 0.48 +0.9 4,876,613 - BWI76_RS23385 0.48 -0.2 4,876,694 + BWI76_RS23385 0.57 +0.2 4,876,798 + BWI76_RS23385 0.68 -0.9 4,877,043 + -1.2 4,877,168 - +0.4
Or see this region's nucleotide sequence