Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23150

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23135 and BWI76_RS23140 are separated by 19 nucleotidesBWI76_RS23140 and BWI76_RS23145 are separated by 24 nucleotidesBWI76_RS23145 and BWI76_RS23150 overlap by 1 nucleotidesBWI76_RS23150 and BWI76_RS23155 overlap by 4 nucleotidesBWI76_RS23155 and BWI76_RS23160 are separated by 3 nucleotidesBWI76_RS23160 and BWI76_RS23165 are separated by 9 nucleotides BWI76_RS23135: BWI76_RS23135 - propanediol dehydratase, at 4,823,419 to 4,823,769 _RS23135 BWI76_RS23140: BWI76_RS23140 - carboxysome shell protein, at 4,823,789 to 4,824,064 _RS23140 BWI76_RS23145: BWI76_RS23145 - propanediol utilization protein, at 4,824,089 to 4,824,550 _RS23145 BWI76_RS23150: BWI76_RS23150 - propanediol utilization phosphotransacylase, at 4,824,550 to 4,825,182 _RS23150 BWI76_RS23155: BWI76_RS23155 - microcompartment protein PduM, at 4,825,179 to 4,825,670 _RS23155 BWI76_RS23160: BWI76_RS23160 - ethanolamine utilization protein EutN, at 4,825,674 to 4,825,949 _RS23160 BWI76_RS23165: BWI76_RS23165 - ATP:cob(I)alamin adenosyltransferase, at 4,825,959 to 4,826,969 _RS23165 Position (kb) 4824 4825 4826Strain fitness (log2 ratio) -1 0 1 2 3at 4823.559 kb on + strand, within BWI76_RS23135at 4823.567 kb on - strand, within BWI76_RS23135at 4823.914 kb on - strand, within BWI76_RS23140at 4823.914 kb on - strand, within BWI76_RS23140at 4824.082 kb on + strandat 4824.675 kb on - strand, within BWI76_RS23150at 4824.841 kb on + strand, within BWI76_RS23150at 4824.879 kb on + strand, within BWI76_RS23150at 4825.221 kb on - strandat 4825.274 kb on + strand, within BWI76_RS23155at 4825.417 kb on - strand, within BWI76_RS23155at 4825.496 kb on - strand, within BWI76_RS23155at 4825.572 kb on + strand, within BWI76_RS23155at 4825.612 kb on + strand, within BWI76_RS23155at 4825.973 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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4,823,559 + BWI76_RS23135 0.40 -0.3
4,823,567 - BWI76_RS23135 0.42 +1.5
4,823,914 - BWI76_RS23140 0.45 +0.1
4,823,914 - BWI76_RS23140 0.45 +2.9
4,824,082 + +0.3
4,824,675 - BWI76_RS23150 0.20 +1.2
4,824,841 + BWI76_RS23150 0.46 +0.4
4,824,879 + BWI76_RS23150 0.52 +0.7
4,825,221 - -0.6
4,825,274 + BWI76_RS23155 0.19 -0.9
4,825,417 - BWI76_RS23155 0.48 +0.3
4,825,496 - BWI76_RS23155 0.64 -0.2
4,825,572 + BWI76_RS23155 0.80 +0.1
4,825,612 + BWI76_RS23155 0.88 +2.7
4,825,973 - -0.3

Or see this region's nucleotide sequence