Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22850 and BWI76_RS22855 overlap by 1 nucleotides BWI76_RS22855 and BWI76_RS22860 are separated by 633 nucleotides BWI76_RS22860 and BWI76_RS22865 are separated by 69 nucleotides
BWI76_RS22850: BWI76_RS22850 - tRNA pseudouridine synthase TruC, at 4,767,545 to 4,768,339
_RS22850
BWI76_RS22855: BWI76_RS22855 - hypothetical protein, at 4,768,339 to 4,768,668
_RS22855
BWI76_RS22860: BWI76_RS22860 - SecY-interacting protein, at 4,769,302 to 4,769,847
_RS22860
BWI76_RS22865: BWI76_RS22865 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 4,769,917 to 4,770,762
_RS22865
Position (kb)
4769
4770 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4768.370 kb on + strand at 4768.428 kb on + strand, within BWI76_RS22855 at 4768.530 kb on + strand, within BWI76_RS22855 at 4768.538 kb on - strand, within BWI76_RS22855 at 4768.610 kb on - strand, within BWI76_RS22855 at 4768.748 kb on - strand at 4768.748 kb on - strand at 4768.803 kb on - strand at 4768.859 kb on - strand at 4768.902 kb on - strand at 4768.904 kb on + strand at 4768.985 kb on - strand at 4769.116 kb on + strand at 4769.378 kb on - strand, within BWI76_RS22860 at 4769.683 kb on - strand, within BWI76_RS22860 at 4769.798 kb on + strand at 4769.826 kb on + strand at 4769.834 kb on - strand at 4769.880 kb on - strand at 4769.948 kb on - strand at 4770.001 kb on - strand at 4770.003 kb on + strand, within BWI76_RS22865 at 4770.011 kb on - strand, within BWI76_RS22865 at 4770.061 kb on - strand, within BWI76_RS22865 at 4770.091 kb on + strand, within BWI76_RS22865 at 4770.099 kb on + strand, within BWI76_RS22865 at 4770.399 kb on + strand, within BWI76_RS22865 at 4770.415 kb on - strand, within BWI76_RS22865 at 4770.442 kb on + strand, within BWI76_RS22865 at 4770.830 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 4,768,370 + -3.5 4,768,428 + BWI76_RS22855 0.27 +0.0 4,768,530 + BWI76_RS22855 0.58 -0.5 4,768,538 - BWI76_RS22855 0.60 +0.2 4,768,610 - BWI76_RS22855 0.82 +3.7 4,768,748 - +3.4 4,768,748 - +3.0 4,768,803 - +1.5 4,768,859 - +2.8 4,768,902 - +3.5 4,768,904 + -0.8 4,768,985 - +2.5 4,769,116 + +2.9 4,769,378 - BWI76_RS22860 0.14 -0.7 4,769,683 - BWI76_RS22860 0.70 -1.4 4,769,798 + -0.8 4,769,826 + -2.8 4,769,834 - +0.2 4,769,880 - -0.4 4,769,948 - +0.7 4,770,001 - +0.4 4,770,003 + BWI76_RS22865 0.10 +0.7 4,770,011 - BWI76_RS22865 0.11 +0.3 4,770,061 - BWI76_RS22865 0.17 -2.2 4,770,091 + BWI76_RS22865 0.21 +0.6 4,770,099 + BWI76_RS22865 0.22 -0.7 4,770,399 + BWI76_RS22865 0.57 +0.4 4,770,415 - BWI76_RS22865 0.59 -1.4 4,770,442 + BWI76_RS22865 0.62 +0.6 4,770,830 + -0.1
Or see this region's nucleotide sequence