Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22720 and BWI76_RS22725 overlap by 4 nucleotides BWI76_RS22725 and BWI76_RS22730 overlap by 19 nucleotides BWI76_RS22730 and BWI76_RS22735 are separated by 9 nucleotides
BWI76_RS22720: BWI76_RS22720 - subtype I-E CRISPR-associated endonuclease Cas1, at 4,734,800 to 4,735,720
_RS22720
BWI76_RS22725: BWI76_RS22725 - type I-E CRISPR-associated protein Cas6/Cse3/CasE, at 4,735,717 to 4,736,367
_RS22725
BWI76_RS22730: BWI76_RS22730 - type I-E CRISPR-associated protein Cas5/CasD, at 4,736,349 to 4,737,095
_RS22730
BWI76_RS22735: BWI76_RS22735 - type I-E CRISPR-associated protein Cas7/Cse4/CasC, at 4,737,105 to 4,738,163
_RS22735
Position (kb) 4736
4737
4738 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4735.708 kb on + strand at 4735.708 kb on + strand at 4735.751 kb on - strand at 4735.751 kb on - strand at 4735.808 kb on - strand, within BWI76_RS22725 at 4735.847 kb on + strand, within BWI76_RS22725 at 4735.855 kb on - strand, within BWI76_RS22725 at 4735.865 kb on - strand, within BWI76_RS22725 at 4735.958 kb on + strand, within BWI76_RS22725 at 4736.003 kb on + strand, within BWI76_RS22725 at 4736.099 kb on - strand, within BWI76_RS22725 at 4736.099 kb on - strand, within BWI76_RS22725 at 4736.104 kb on + strand, within BWI76_RS22725 at 4736.104 kb on + strand, within BWI76_RS22725 at 4736.183 kb on - strand, within BWI76_RS22725 at 4736.187 kb on + strand, within BWI76_RS22725 at 4736.207 kb on + strand, within BWI76_RS22725 at 4736.329 kb on - strand at 4736.362 kb on + strand at 4736.370 kb on - strand at 4736.462 kb on - strand, within BWI76_RS22730 at 4736.463 kb on - strand, within BWI76_RS22730 at 4736.574 kb on + strand, within BWI76_RS22730 at 4736.680 kb on - strand, within BWI76_RS22730 at 4736.752 kb on + strand, within BWI76_RS22730 at 4736.760 kb on - strand, within BWI76_RS22730 at 4736.784 kb on + strand, within BWI76_RS22730 at 4736.789 kb on + strand, within BWI76_RS22730 at 4736.813 kb on - strand, within BWI76_RS22730 at 4736.885 kb on + strand, within BWI76_RS22730 at 4736.909 kb on - strand, within BWI76_RS22730 at 4736.990 kb on - strand, within BWI76_RS22730 at 4737.010 kb on - strand, within BWI76_RS22730 at 4737.129 kb on + strand at 4737.166 kb on - strand at 4737.215 kb on + strand, within BWI76_RS22735 at 4737.215 kb on + strand, within BWI76_RS22735 at 4737.215 kb on + strand, within BWI76_RS22735 at 4737.478 kb on + strand, within BWI76_RS22735 at 4737.602 kb on + strand, within BWI76_RS22735 at 4737.610 kb on - strand at 4737.806 kb on - strand, within BWI76_RS22735 at 4737.956 kb on + strand, within BWI76_RS22735 at 4738.074 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 4,735,708 + -0.8 4,735,708 + +0.9 4,735,751 - -0.5 4,735,751 - +0.2 4,735,808 - BWI76_RS22725 0.14 +0.7 4,735,847 + BWI76_RS22725 0.20 -0.7 4,735,855 - BWI76_RS22725 0.21 +0.5 4,735,865 - BWI76_RS22725 0.23 -1.1 4,735,958 + BWI76_RS22725 0.37 +0.4 4,736,003 + BWI76_RS22725 0.44 +0.3 4,736,099 - BWI76_RS22725 0.59 -0.4 4,736,099 - BWI76_RS22725 0.59 -0.0 4,736,104 + BWI76_RS22725 0.59 -0.6 4,736,104 + BWI76_RS22725 0.59 -0.9 4,736,183 - BWI76_RS22725 0.72 -1.2 4,736,187 + BWI76_RS22725 0.72 -0.4 4,736,207 + BWI76_RS22725 0.75 +1.2 4,736,329 - -1.6 4,736,362 + -1.2 4,736,370 - +0.2 4,736,462 - BWI76_RS22730 0.15 -1.2 4,736,463 - BWI76_RS22730 0.15 +0.4 4,736,574 + BWI76_RS22730 0.30 -0.3 4,736,680 - BWI76_RS22730 0.44 -0.5 4,736,752 + BWI76_RS22730 0.54 -0.8 4,736,760 - BWI76_RS22730 0.55 -1.5 4,736,784 + BWI76_RS22730 0.58 -3.0 4,736,789 + BWI76_RS22730 0.59 -0.8 4,736,813 - BWI76_RS22730 0.62 +0.5 4,736,885 + BWI76_RS22730 0.72 -0.6 4,736,909 - BWI76_RS22730 0.75 +0.4 4,736,990 - BWI76_RS22730 0.86 -0.5 4,737,010 - BWI76_RS22730 0.88 -0.2 4,737,129 + -1.1 4,737,166 - -0.8 4,737,215 + BWI76_RS22735 0.10 +0.5 4,737,215 + BWI76_RS22735 0.10 +0.7 4,737,215 + BWI76_RS22735 0.10 +0.0 4,737,478 + BWI76_RS22735 0.35 +0.1 4,737,602 + BWI76_RS22735 0.47 +0.6 4,737,610 - +1.4 4,737,806 - BWI76_RS22735 0.66 -0.0 4,737,956 + BWI76_RS22735 0.80 +0.4 4,738,074 - +0.2
Or see this region's nucleotide sequence