Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21075

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21070 and BWI76_RS21075 are separated by 10 nucleotidesBWI76_RS21075 and BWI76_RS21080 are separated by 34 nucleotides BWI76_RS21070: BWI76_RS21070 - outer membrane protein assembly factor BamB, at 4,408,039 to 4,409,217 _RS21070 BWI76_RS21075: BWI76_RS21075 - membrane protein, at 4,409,228 to 4,409,848 _RS21075 BWI76_RS21080: BWI76_RS21080 - histidine--tRNA ligase, at 4,409,883 to 4,411,157 _RS21080 Position (kb) 4409 4410Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 4408.334 kb on - strand, within BWI76_RS21070at 4408.461 kb on - strand, within BWI76_RS21070at 4409.039 kb on - strand, within BWI76_RS21070at 4409.392 kb on - strand, within BWI76_RS21075at 4409.438 kb on - strand, within BWI76_RS21075at 4409.713 kb on - strand, within BWI76_RS21075at 4409.894 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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4,408,334 - BWI76_RS21070 0.25 +4.6
4,408,461 - BWI76_RS21070 0.36 +5.8
4,409,039 - BWI76_RS21070 0.85 +4.5
4,409,392 - BWI76_RS21075 0.26 +1.0
4,409,438 - BWI76_RS21075 0.34 +1.0
4,409,713 - BWI76_RS21075 0.78 +3.6
4,409,894 - +1.3

Or see this region's nucleotide sequence