Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20635

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20630 and BWI76_RS20635 are separated by 230 nucleotidesBWI76_RS20635 and BWI76_RS20640 are separated by 159 nucleotides BWI76_RS20630: BWI76_RS20630 - cell division protein ZipA, at 4,317,061 to 4,318,056 _RS20630 BWI76_RS20635: BWI76_RS20635 - sulfate transporter CysZ, at 4,318,287 to 4,319,048 _RS20635 BWI76_RS20640: BWI76_RS20640 - cysteine synthase A, at 4,319,208 to 4,320,179 _RS20640 Position (kb) 4318 4319 4320Strain fitness (log2 ratio) -3 -2 -1 0 1at 4318.350 kb on - strandat 4318.443 kb on + strand, within BWI76_RS20635at 4318.539 kb on - strand, within BWI76_RS20635at 4318.624 kb on - strand, within BWI76_RS20635at 4318.864 kb on - strand, within BWI76_RS20635at 4318.897 kb on + strand, within BWI76_RS20635at 4318.970 kb on - strand, within BWI76_RS20635at 4319.067 kb on + strandat 4319.160 kb on + strandat 4319.202 kb on - strandat 4319.239 kb on + strandat 4319.461 kb on + strand, within BWI76_RS20640at 4319.469 kb on - strand, within BWI76_RS20640at 4319.483 kb on + strand, within BWI76_RS20640at 4319.507 kb on + strand, within BWI76_RS20640at 4319.530 kb on + strand, within BWI76_RS20640at 4319.820 kb on - strand, within BWI76_RS20640at 4319.865 kb on - strand, within BWI76_RS20640

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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4,318,350 - +0.0
4,318,443 + BWI76_RS20635 0.20 +1.2
4,318,539 - BWI76_RS20635 0.33 -1.3
4,318,624 - BWI76_RS20635 0.44 -0.4
4,318,864 - BWI76_RS20635 0.76 -1.9
4,318,897 + BWI76_RS20635 0.80 -2.8
4,318,970 - BWI76_RS20635 0.90 -1.0
4,319,067 + +0.4
4,319,160 + +0.2
4,319,202 - -0.3
4,319,239 + -2.1
4,319,461 + BWI76_RS20640 0.26 +0.1
4,319,469 - BWI76_RS20640 0.27 +0.4
4,319,483 + BWI76_RS20640 0.28 -1.1
4,319,507 + BWI76_RS20640 0.31 +0.0
4,319,530 + BWI76_RS20640 0.33 -0.7
4,319,820 - BWI76_RS20640 0.63 -0.6
4,319,865 - BWI76_RS20640 0.68 +0.9

Or see this region's nucleotide sequence