Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20435

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20425 and BWI76_RS20430 overlap by 1 nucleotidesBWI76_RS20430 and BWI76_RS20435 are separated by 3 nucleotidesBWI76_RS20435 and BWI76_RS20440 are separated by 37 nucleotides BWI76_RS20425: BWI76_RS20425 - hypothetical protein, at 4,275,689 to 4,276,498 _RS20425 BWI76_RS20430: BWI76_RS20430 - penicillin-insensitive murein endopeptidase, at 4,276,498 to 4,277,322 _RS20430 BWI76_RS20435: BWI76_RS20435 - chorismate synthase, at 4,277,326 to 4,278,411 _RS20435 BWI76_RS20440: BWI76_RS20440 - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 4,278,449 to 4,279,381 _RS20440 Position (kb) 4277 4278 4279Strain fitness (log2 ratio) -2 -1 0 1at 4276.504 kb on + strandat 4277.065 kb on - strand, within BWI76_RS20430at 4277.133 kb on - strand, within BWI76_RS20430at 4277.528 kb on + strand, within BWI76_RS20435at 4278.248 kb on - strand, within BWI76_RS20435at 4278.348 kb on - strandat 4278.588 kb on - strand, within BWI76_RS20440at 4278.639 kb on + strand, within BWI76_RS20440at 4278.867 kb on + strand, within BWI76_RS20440at 4278.867 kb on + strand, within BWI76_RS20440at 4279.395 kb on - strandat 4279.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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4,276,504 + -0.2
4,277,065 - BWI76_RS20430 0.69 +0.5
4,277,133 - BWI76_RS20430 0.77 +0.7
4,277,528 + BWI76_RS20435 0.19 -0.4
4,278,248 - BWI76_RS20435 0.85 -1.7
4,278,348 - +0.5
4,278,588 - BWI76_RS20440 0.15 -0.6
4,278,639 + BWI76_RS20440 0.20 -0.2
4,278,867 + BWI76_RS20440 0.45 -2.0
4,278,867 + BWI76_RS20440 0.45 -2.4
4,279,395 - +0.7
4,279,396 - +0.6

Or see this region's nucleotide sequence