Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20075 and BWI76_RS20080 are separated by 77 nucleotides BWI76_RS20080 and BWI76_RS20085 are separated by 67 nucleotides BWI76_RS20085 and BWI76_RS20090 are separated by 60 nucleotides
BWI76_RS20075: BWI76_RS20075 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase, at 4,205,196 to 4,206,866
_RS20075
BWI76_RS20080: BWI76_RS20080 - isochorismate synthase MenF, at 4,206,944 to 4,208,269
_RS20080
BWI76_RS20085: BWI76_RS20085 - GNAT family N-acetyltransferase, at 4,208,337 to 4,208,798
_RS20085
BWI76_RS20090: BWI76_RS20090 - ribonuclease Z, at 4,208,859 to 4,209,776
_RS20090
Position (kb)
4206
4207
4208
4209 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4206.257 kb on - strand, within BWI76_RS20075 at 4206.268 kb on + strand, within BWI76_RS20075 at 4206.414 kb on + strand, within BWI76_RS20075 at 4206.612 kb on - strand, within BWI76_RS20075 at 4206.761 kb on - strand at 4206.830 kb on + strand at 4206.845 kb on + strand at 4206.904 kb on - strand at 4207.023 kb on - strand at 4207.563 kb on - strand, within BWI76_RS20080 at 4207.664 kb on + strand, within BWI76_RS20080 at 4207.671 kb on + strand, within BWI76_RS20080 at 4207.872 kb on - strand, within BWI76_RS20080 at 4207.911 kb on - strand, within BWI76_RS20080 at 4207.911 kb on - strand, within BWI76_RS20080 at 4208.076 kb on + strand, within BWI76_RS20080 at 4208.122 kb on + strand, within BWI76_RS20080 at 4208.203 kb on + strand at 4208.408 kb on + strand, within BWI76_RS20085 at 4208.525 kb on - strand, within BWI76_RS20085 at 4208.743 kb on + strand, within BWI76_RS20085 at 4208.799 kb on - strand at 4208.923 kb on - strand at 4209.076 kb on + strand, within BWI76_RS20090 at 4209.110 kb on - strand, within BWI76_RS20090 at 4209.144 kb on - strand, within BWI76_RS20090 at 4209.223 kb on + strand, within BWI76_RS20090
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 4,206,257 - BWI76_RS20075 0.63 +0.1 4,206,268 + BWI76_RS20075 0.64 +0.4 4,206,414 + BWI76_RS20075 0.73 -0.2 4,206,612 - BWI76_RS20075 0.85 +0.4 4,206,761 - +0.8 4,206,830 + +0.7 4,206,845 + +1.3 4,206,904 - -1.5 4,207,023 - -0.4 4,207,563 - BWI76_RS20080 0.47 +1.7 4,207,664 + BWI76_RS20080 0.54 -2.1 4,207,671 + BWI76_RS20080 0.55 +1.7 4,207,872 - BWI76_RS20080 0.70 +0.7 4,207,911 - BWI76_RS20080 0.73 +0.9 4,207,911 - BWI76_RS20080 0.73 +0.7 4,208,076 + BWI76_RS20080 0.85 +2.5 4,208,122 + BWI76_RS20080 0.89 -1.0 4,208,203 + +1.0 4,208,408 + BWI76_RS20085 0.15 +0.4 4,208,525 - BWI76_RS20085 0.41 -0.5 4,208,743 + BWI76_RS20085 0.88 +1.1 4,208,799 - -1.0 4,208,923 - +0.7 4,209,076 + BWI76_RS20090 0.24 +1.3 4,209,110 - BWI76_RS20090 0.27 +1.7 4,209,144 - BWI76_RS20090 0.31 +2.5 4,209,223 + BWI76_RS20090 0.40 -2.2
Or see this region's nucleotide sequence