Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS19220

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS19210 and BWI76_RS19215 are separated by 84 nucleotidesBWI76_RS19215 and BWI76_RS19220 overlap by 29 nucleotidesBWI76_RS19220 and BWI76_RS19225 are separated by 14 nucleotidesBWI76_RS19225 and BWI76_RS19230 are separated by 0 nucleotides BWI76_RS19210: BWI76_RS19210 - LuxR family transcriptional regulator, at 3,998,342 to 3,999,094 _RS19210 BWI76_RS19215: BWI76_RS19215 - proline-specific peptidase, at 3,999,179 to 4,000,111 _RS19215 BWI76_RS19220: BWI76_RS19220 - hypothetical protein, at 4,000,083 to 4,001,201 _RS19220 BWI76_RS19225: BWI76_RS19225 - AsnC family transcriptional regulator, at 4,001,216 to 4,001,671 _RS19225 BWI76_RS19230: BWI76_RS19230 - polar amino acid ABC transporter inner membrane subunit, at 4,001,672 to 4,002,397 _RS19230 Position (kb) 4000 4001 4002Strain fitness (log2 ratio) -2 -1 0 1 2at 3999.134 kb on + strandat 3999.570 kb on - strand, within BWI76_RS19215at 4000.261 kb on + strand, within BWI76_RS19220at 4000.475 kb on + strand, within BWI76_RS19220at 4000.475 kb on + strand, within BWI76_RS19220at 4000.760 kb on - strand, within BWI76_RS19220at 4000.969 kb on + strand, within BWI76_RS19220at 4001.044 kb on + strand, within BWI76_RS19220at 4001.319 kb on - strand, within BWI76_RS19225at 4001.345 kb on - strand, within BWI76_RS19225at 4001.401 kb on + strand, within BWI76_RS19225at 4001.570 kb on + strand, within BWI76_RS19225at 4001.596 kb on - strand, within BWI76_RS19225at 4001.845 kb on - strandat 4001.930 kb on - strand, within BWI76_RS19230

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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3,999,134 + -2.0
3,999,570 - BWI76_RS19215 0.42 -0.5
4,000,261 + BWI76_RS19220 0.16 -0.6
4,000,475 + BWI76_RS19220 0.35 -2.3
4,000,475 + BWI76_RS19220 0.35 +0.8
4,000,760 - BWI76_RS19220 0.61 -1.6
4,000,969 + BWI76_RS19220 0.79 +0.0
4,001,044 + BWI76_RS19220 0.86 +0.5
4,001,319 - BWI76_RS19225 0.23 -0.3
4,001,345 - BWI76_RS19225 0.28 -0.6
4,001,401 + BWI76_RS19225 0.41 -0.0
4,001,570 + BWI76_RS19225 0.78 -0.1
4,001,596 - BWI76_RS19225 0.83 -1.8
4,001,845 - +2.0
4,001,930 - BWI76_RS19230 0.36 -1.3

Or see this region's nucleotide sequence