Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17905 and BWI76_RS17910 are separated by 106 nucleotides BWI76_RS17910 and BWI76_RS17915 are separated by 96 nucleotides BWI76_RS17915 and BWI76_RS17920 are separated by 3 nucleotides BWI76_RS17920 and BWI76_RS17925 are separated by 60 nucleotides
BWI76_RS17905: BWI76_RS17905 - hypothetical protein, at 3,724,326 to 3,724,784
_RS17905
BWI76_RS17910: BWI76_RS17910 - hypothetical protein, at 3,724,891 to 3,725,112
_RS17910
BWI76_RS17915: BWI76_RS17915 - membrane protein, at 3,725,209 to 3,725,775
_RS17915
BWI76_RS17920: BWI76_RS17920 - 23S rRNA (guanine(745)-N(1))-methyltransferase, at 3,725,779 to 3,726,573
_RS17920
BWI76_RS17925: BWI76_RS17925 - peptidoglycan synthase, at 3,726,634 to 3,728,379
_RS17925
Position (kb)
3725
3726
3727 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3724.801 kb on + strand at 3724.806 kb on + strand at 3724.806 kb on + strand at 3724.806 kb on + strand at 3724.806 kb on + strand at 3724.835 kb on - strand at 3724.840 kb on - strand at 3724.980 kb on + strand, within BWI76_RS17910 at 3725.035 kb on - strand, within BWI76_RS17910 at 3725.063 kb on - strand, within BWI76_RS17910 at 3725.069 kb on + strand, within BWI76_RS17910 at 3725.074 kb on + strand, within BWI76_RS17910 at 3725.077 kb on - strand, within BWI76_RS17910 at 3725.077 kb on - strand, within BWI76_RS17910 at 3725.077 kb on - strand, within BWI76_RS17910 at 3725.089 kb on + strand, within BWI76_RS17910 at 3725.099 kb on + strand at 3725.099 kb on + strand at 3725.108 kb on - strand at 3725.109 kb on - strand at 3725.110 kb on + strand at 3725.118 kb on - strand at 3725.118 kb on - strand at 3725.189 kb on + strand at 3725.326 kb on + strand, within BWI76_RS17915 at 3725.334 kb on - strand, within BWI76_RS17915 at 3725.334 kb on - strand, within BWI76_RS17915 at 3725.576 kb on + strand, within BWI76_RS17915 at 3725.623 kb on + strand, within BWI76_RS17915 at 3725.631 kb on - strand, within BWI76_RS17915 at 3725.728 kb on - strand at 3725.743 kb on + strand at 3725.926 kb on - strand, within BWI76_RS17920 at 3725.979 kb on + strand, within BWI76_RS17920 at 3726.603 kb on + strand at 3726.611 kb on - strand at 3726.993 kb on - strand, within BWI76_RS17925 at 3727.153 kb on + strand, within BWI76_RS17925 at 3727.353 kb on + strand, within BWI76_RS17925 at 3727.368 kb on - strand, within BWI76_RS17925
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 3,724,801 + +0.9 3,724,806 + -2.7 3,724,806 + +0.2 3,724,806 + -0.8 3,724,806 + -2.4 3,724,835 - -1.6 3,724,840 - +1.6 3,724,980 + BWI76_RS17910 0.40 +0.1 3,725,035 - BWI76_RS17910 0.65 -0.1 3,725,063 - BWI76_RS17910 0.77 +2.2 3,725,069 + BWI76_RS17910 0.80 -0.6 3,725,074 + BWI76_RS17910 0.82 -2.4 3,725,077 - BWI76_RS17910 0.84 +0.5 3,725,077 - BWI76_RS17910 0.84 +1.8 3,725,077 - BWI76_RS17910 0.84 -1.0 3,725,089 + BWI76_RS17910 0.89 -0.6 3,725,099 + +2.2 3,725,099 + +0.1 3,725,108 - -2.2 3,725,109 - -2.6 3,725,110 + +1.2 3,725,118 - +0.8 3,725,118 - +0.5 3,725,189 + -2.2 3,725,326 + BWI76_RS17915 0.21 +1.1 3,725,334 - BWI76_RS17915 0.22 -3.3 3,725,334 - BWI76_RS17915 0.22 -0.2 3,725,576 + BWI76_RS17915 0.65 -0.3 3,725,623 + BWI76_RS17915 0.73 +1.9 3,725,631 - BWI76_RS17915 0.74 +0.7 3,725,728 - +0.6 3,725,743 + +0.0 3,725,926 - BWI76_RS17920 0.18 -1.6 3,725,979 + BWI76_RS17920 0.25 -1.4 3,726,603 + -1.0 3,726,611 - -1.7 3,726,993 - BWI76_RS17925 0.21 +0.5 3,727,153 + BWI76_RS17925 0.30 +1.4 3,727,353 + BWI76_RS17925 0.41 -0.8 3,727,368 - BWI76_RS17925 0.42 -2.2
Or see this region's nucleotide sequence