Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17175

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17170 and BWI76_RS17175 are separated by 289 nucleotidesBWI76_RS17175 and BWI76_RS17180 are separated by 92 nucleotidesBWI76_RS17180 and BWI76_RS17185 are separated by 106 nucleotides BWI76_RS17170: BWI76_RS17170 - membrane protein, at 3,558,961 to 3,559,719 _RS17170 BWI76_RS17175: BWI76_RS17175 - 6-phosphofructokinase II, at 3,560,009 to 3,560,941 _RS17175 BWI76_RS17180: BWI76_RS17180 - hypothetical protein, at 3,561,034 to 3,561,318 _RS17180 BWI76_RS17185: BWI76_RS17185 - fructosamine kinase family protein, at 3,561,425 to 3,562,297 _RS17185 Position (kb) 3560 3561Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3559.013 kb on - strandat 3559.016 kb on + strandat 3559.031 kb on + strandat 3559.034 kb on - strandat 3559.044 kb on + strand, within BWI76_RS17170at 3559.053 kb on - strand, within BWI76_RS17170at 3559.055 kb on + strand, within BWI76_RS17170at 3559.087 kb on + strand, within BWI76_RS17170at 3559.087 kb on + strand, within BWI76_RS17170at 3559.087 kb on + strand, within BWI76_RS17170at 3559.105 kb on - strand, within BWI76_RS17170at 3559.105 kb on - strand, within BWI76_RS17170at 3559.146 kb on + strand, within BWI76_RS17170at 3559.248 kb on + strand, within BWI76_RS17170at 3559.278 kb on + strand, within BWI76_RS17170at 3559.311 kb on - strand, within BWI76_RS17170at 3559.348 kb on - strand, within BWI76_RS17170at 3559.398 kb on + strand, within BWI76_RS17170at 3559.514 kb on + strand, within BWI76_RS17170at 3559.615 kb on - strand, within BWI76_RS17170at 3559.626 kb on - strand, within BWI76_RS17170at 3559.750 kb on + strandat 3559.753 kb on - strandat 3559.770 kb on - strandat 3559.852 kb on - strandat 3559.874 kb on + strandat 3560.031 kb on + strandat 3560.039 kb on - strandat 3560.041 kb on + strandat 3560.140 kb on + strand, within BWI76_RS17175at 3560.934 kb on + strandat 3561.067 kb on + strand, within BWI76_RS17180at 3561.144 kb on - strand, within BWI76_RS17180at 3561.261 kb on + strand, within BWI76_RS17180at 3561.767 kb on + strand, within BWI76_RS17185

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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3,559,013 - -0.3
3,559,016 + -0.1
3,559,031 + +3.2
3,559,034 - -0.4
3,559,044 + BWI76_RS17170 0.11 -2.4
3,559,053 - BWI76_RS17170 0.12 +0.5
3,559,055 + BWI76_RS17170 0.12 -2.5
3,559,087 + BWI76_RS17170 0.17 -0.6
3,559,087 + BWI76_RS17170 0.17 -0.2
3,559,087 + BWI76_RS17170 0.17 +2.4
3,559,105 - BWI76_RS17170 0.19 -2.6
3,559,105 - BWI76_RS17170 0.19 -0.3
3,559,146 + BWI76_RS17170 0.24 -1.3
3,559,248 + BWI76_RS17170 0.38 -3.1
3,559,278 + BWI76_RS17170 0.42 -1.6
3,559,311 - BWI76_RS17170 0.46 -0.7
3,559,348 - BWI76_RS17170 0.51 -1.1
3,559,398 + BWI76_RS17170 0.58 +2.5
3,559,514 + BWI76_RS17170 0.73 -0.2
3,559,615 - BWI76_RS17170 0.86 -2.1
3,559,626 - BWI76_RS17170 0.88 +0.4
3,559,750 + -0.5
3,559,753 - -0.1
3,559,770 - -1.8
3,559,852 - +1.9
3,559,874 + +0.2
3,560,031 + -1.2
3,560,039 - +0.3
3,560,041 + +0.1
3,560,140 + BWI76_RS17175 0.14 +2.2
3,560,934 + -0.9
3,561,067 + BWI76_RS17180 0.12 -0.5
3,561,144 - BWI76_RS17180 0.39 -0.2
3,561,261 + BWI76_RS17180 0.80 -0.9
3,561,767 + BWI76_RS17185 0.39 +0.7

Or see this region's nucleotide sequence