Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16705 and BWI76_RS16710 are separated by 496 nucleotides BWI76_RS16710 and BWI76_RS16715 are separated by 66 nucleotides BWI76_RS16715 and BWI76_RS16720 are separated by 725 nucleotides
BWI76_RS16705: BWI76_RS16705 - glucose dehydrogenase, at 3,457,562 to 3,459,967
_RS16705
BWI76_RS16710: BWI76_RS16710 - cyclic nucleotide-binding protein, at 3,460,464 to 3,460,693
_RS16710
BWI76_RS16715: BWI76_RS16715 - hypothetical protein, at 3,460,760 to 3,461,668
_RS16715
BWI76_RS16720: BWI76_RS16720 - hypothetical protein, at 3,462,394 to 3,463,731
_RS16720
Position (kb)
3460
3461
3462 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 3459.771 kb on - strand at 3459.803 kb on - strand at 3459.847 kb on - strand at 3460.060 kb on - strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.072 kb on + strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.080 kb on - strand at 3460.252 kb on + strand at 3460.258 kb on - strand at 3460.354 kb on + strand at 3460.391 kb on - strand at 3460.416 kb on + strand at 3460.419 kb on + strand at 3460.424 kb on - strand at 3460.424 kb on - strand at 3460.470 kb on - strand at 3460.481 kb on - strand at 3460.520 kb on + strand, within BWI76_RS16710 at 3460.528 kb on - strand at 3460.637 kb on - strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.647 kb on + strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.655 kb on - strand, within BWI76_RS16710 at 3460.664 kb on - strand at 3460.693 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.698 kb on + strand at 3460.725 kb on + strand at 3460.728 kb on - strand at 3460.733 kb on - strand at 3460.812 kb on - strand at 3460.813 kb on - strand at 3460.821 kb on - strand at 3460.830 kb on - strand at 3460.831 kb on - strand at 3460.831 kb on - strand at 3460.863 kb on + strand at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.871 kb on - strand, within BWI76_RS16715 at 3460.881 kb on - strand, within BWI76_RS16715 at 3460.920 kb on + strand at 3460.975 kb on - strand, within BWI76_RS16715 at 3461.054 kb on - strand, within BWI76_RS16715 at 3461.054 kb on - strand, within BWI76_RS16715 at 3461.085 kb on + strand, within BWI76_RS16715 at 3461.093 kb on - strand, within BWI76_RS16715 at 3461.093 kb on - strand, within BWI76_RS16715 at 3461.093 kb on - strand, within BWI76_RS16715 at 3461.201 kb on - strand, within BWI76_RS16715 at 3461.219 kb on + strand, within BWI76_RS16715 at 3461.219 kb on + strand, within BWI76_RS16715 at 3461.227 kb on - strand, within BWI76_RS16715 at 3461.266 kb on + strand, within BWI76_RS16715 at 3461.272 kb on + strand, within BWI76_RS16715 at 3461.272 kb on + strand, within BWI76_RS16715 at 3461.280 kb on - strand, within BWI76_RS16715 at 3461.280 kb on - strand, within BWI76_RS16715 at 3461.313 kb on - strand, within BWI76_RS16715 at 3461.326 kb on - strand, within BWI76_RS16715 at 3461.326 kb on - strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.334 kb on + strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.342 kb on - strand, within BWI76_RS16715 at 3461.398 kb on - strand, within BWI76_RS16715 at 3461.398 kb on - strand, within BWI76_RS16715 at 3461.442 kb on + strand, within BWI76_RS16715 at 3461.482 kb on - strand, within BWI76_RS16715 at 3461.497 kb on - strand, within BWI76_RS16715 at 3461.512 kb on - strand, within BWI76_RS16715 at 3461.562 kb on - strand, within BWI76_RS16715 at 3461.604 kb on - strand at 3461.655 kb on - strand at 3461.655 kb on - strand at 3461.673 kb on - strand at 3461.738 kb on - strand at 3461.868 kb on - strand at 3461.883 kb on + strand at 3461.883 kb on + strand at 3461.883 kb on + strand at 3461.883 kb on + strand at 3461.918 kb on - strand at 3461.923 kb on - strand at 3461.930 kb on + strand at 3462.033 kb on + strand at 3462.083 kb on + strand at 3462.306 kb on - strand at 3462.322 kb on + strand at 3462.498 kb on + strand at 3462.621 kb on + strand, within BWI76_RS16720 at 3462.629 kb on - strand, within BWI76_RS16720
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 3,459,771 - +2.2 3,459,803 - -2.7 3,459,847 - +1.5 3,460,060 - -0.2 3,460,072 + -2.1 3,460,072 + -2.7 3,460,072 + +3.2 3,460,072 + +2.2 3,460,072 + +0.8 3,460,072 + +0.7 3,460,080 - +2.0 3,460,080 - +0.6 3,460,080 - +0.8 3,460,080 - -0.8 3,460,252 + +1.2 3,460,258 - -1.3 3,460,354 + -0.4 3,460,391 - +1.4 3,460,416 + +1.8 3,460,419 + +2.2 3,460,424 - +0.2 3,460,424 - +0.3 3,460,470 - -3.4 3,460,481 - -0.3 3,460,520 + BWI76_RS16710 0.24 +0.9 3,460,528 - -1.0 3,460,637 - BWI76_RS16710 0.75 -3.6 3,460,647 + BWI76_RS16710 0.80 -0.4 3,460,647 + BWI76_RS16710 0.80 +0.7 3,460,647 + BWI76_RS16710 0.80 -2.1 3,460,647 + BWI76_RS16710 0.80 +2.2 3,460,647 + BWI76_RS16710 0.80 +0.4 3,460,647 + BWI76_RS16710 0.80 +0.6 3,460,647 + BWI76_RS16710 0.80 +1.0 3,460,647 + BWI76_RS16710 0.80 +0.0 3,460,655 - BWI76_RS16710 0.83 +0.9 3,460,655 - BWI76_RS16710 0.83 -0.9 3,460,655 - BWI76_RS16710 0.83 -0.6 3,460,655 - BWI76_RS16710 0.83 +0.2 3,460,655 - BWI76_RS16710 0.83 -0.3 3,460,655 - BWI76_RS16710 0.83 -0.8 3,460,655 - BWI76_RS16710 0.83 -1.6 3,460,655 - BWI76_RS16710 0.83 +0.3 3,460,655 - BWI76_RS16710 0.83 -0.5 3,460,655 - BWI76_RS16710 0.83 +1.6 3,460,655 - BWI76_RS16710 0.83 +1.2 3,460,664 - -0.4 3,460,693 + -1.3 3,460,698 + +0.7 3,460,698 + -0.5 3,460,698 + -0.1 3,460,698 + +3.8 3,460,698 + -2.0 3,460,725 + -0.1 3,460,728 - +7.2 3,460,733 - +5.9 3,460,812 - +8.0 3,460,813 - +7.6 3,460,821 - +7.5 3,460,830 - +7.6 3,460,831 - +6.6 3,460,831 - +6.0 3,460,863 + +0.2 3,460,871 - BWI76_RS16715 0.12 +7.7 3,460,871 - BWI76_RS16715 0.12 +6.7 3,460,871 - BWI76_RS16715 0.12 +6.3 3,460,881 - BWI76_RS16715 0.13 +6.8 3,460,920 + +5.3 3,460,975 - BWI76_RS16715 0.24 +7.8 3,461,054 - BWI76_RS16715 0.32 +6.8 3,461,054 - BWI76_RS16715 0.32 +8.2 3,461,085 + BWI76_RS16715 0.36 +7.5 3,461,093 - BWI76_RS16715 0.37 +6.5 3,461,093 - BWI76_RS16715 0.37 +7.5 3,461,093 - BWI76_RS16715 0.37 +8.1 3,461,201 - BWI76_RS16715 0.49 +7.9 3,461,219 + BWI76_RS16715 0.50 +8.5 3,461,219 + BWI76_RS16715 0.50 +2.2 3,461,227 - BWI76_RS16715 0.51 +6.9 3,461,266 + BWI76_RS16715 0.56 +6.9 3,461,272 + BWI76_RS16715 0.56 +7.1 3,461,272 + BWI76_RS16715 0.56 +6.7 3,461,280 - BWI76_RS16715 0.57 +7.0 3,461,280 - BWI76_RS16715 0.57 +8.4 3,461,313 - BWI76_RS16715 0.61 +8.5 3,461,326 - BWI76_RS16715 0.62 +7.8 3,461,326 - BWI76_RS16715 0.62 +6.9 3,461,334 + BWI76_RS16715 0.63 +5.5 3,461,334 + BWI76_RS16715 0.63 +0.6 3,461,334 + BWI76_RS16715 0.63 +7.6 3,461,334 + BWI76_RS16715 0.63 +10.7 3,461,342 - BWI76_RS16715 0.64 +6.2 3,461,342 - BWI76_RS16715 0.64 +7.3 3,461,342 - BWI76_RS16715 0.64 +5.9 3,461,342 - BWI76_RS16715 0.64 +6.5 3,461,342 - BWI76_RS16715 0.64 +6.8 3,461,398 - BWI76_RS16715 0.70 +6.5 3,461,398 - BWI76_RS16715 0.70 +9.2 3,461,442 + BWI76_RS16715 0.75 +7.5 3,461,482 - BWI76_RS16715 0.79 +7.6 3,461,497 - BWI76_RS16715 0.81 +7.1 3,461,512 - BWI76_RS16715 0.83 +6.9 3,461,562 - BWI76_RS16715 0.88 +6.8 3,461,604 - +7.4 3,461,655 - +6.4 3,461,655 - +6.5 3,461,673 - +6.7 3,461,738 - +6.5 3,461,868 - +5.0 3,461,883 + +6.1 3,461,883 + +4.6 3,461,883 + +4.5 3,461,883 + +5.3 3,461,918 - +6.0 3,461,923 - +4.0 3,461,930 + +3.8 3,462,033 + +2.9 3,462,083 + +2.2 3,462,306 - +2.8 3,462,322 + +2.0 3,462,498 + +2.2 3,462,621 + BWI76_RS16720 0.17 -0.1 3,462,629 - BWI76_RS16720 0.18 +2.9
Or see this region's nucleotide sequence