Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16330 and BWI76_RS16335 are separated by 177 nucleotides BWI76_RS16335 and BWI76_RS16340 are separated by 103 nucleotides BWI76_RS16340 and BWI76_RS16345 are separated by 220 nucleotides BWI76_RS16345 and BWI76_RS16350 are separated by 79 nucleotides
BWI76_RS16330: BWI76_RS16330 - oxidoreductase, at 3,389,727 to 3,390,767
_RS16330
BWI76_RS16335: BWI76_RS16335 - division septum protein Blr, at 3,390,945 to 3,391,094
_RS16335
BWI76_RS16340: BWI76_RS16340 - aryl-phospho-beta-D-glucosidase, at 3,391,198 to 3,392,568
_RS16340
BWI76_RS16345: BWI76_RS16345 - nucleoid-associated protein, at 3,392,789 to 3,393,004
_RS16345
BWI76_RS16350: BWI76_RS16350 - membrane protein, at 3,393,084 to 3,393,524
_RS16350
Position (kb)
3391
3392
3393 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3390.199 kb on - strand, within BWI76_RS16330 at 3390.437 kb on - strand, within BWI76_RS16330 at 3390.553 kb on - strand, within BWI76_RS16330 at 3390.856 kb on - strand at 3391.018 kb on - strand, within BWI76_RS16335 at 3391.100 kb on + strand at 3391.237 kb on + strand at 3391.303 kb on - strand at 3391.330 kb on - strand at 3392.157 kb on - strand, within BWI76_RS16340 at 3392.193 kb on + strand, within BWI76_RS16340 at 3392.214 kb on + strand, within BWI76_RS16340 at 3392.354 kb on - strand, within BWI76_RS16340 at 3392.390 kb on + strand, within BWI76_RS16340 at 3392.393 kb on - strand, within BWI76_RS16340 at 3392.398 kb on - strand, within BWI76_RS16340 at 3392.413 kb on - strand, within BWI76_RS16340 at 3392.414 kb on + strand, within BWI76_RS16340 at 3392.415 kb on - strand, within BWI76_RS16340 at 3392.472 kb on + strand at 3392.480 kb on - strand at 3392.562 kb on + strand at 3392.562 kb on + strand at 3392.617 kb on - strand at 3392.718 kb on + strand at 3392.723 kb on + strand at 3392.728 kb on + strand at 3392.728 kb on + strand at 3392.731 kb on - strand at 3392.736 kb on + strand at 3392.736 kb on - strand at 3392.743 kb on + strand at 3392.863 kb on + strand, within BWI76_RS16345 at 3392.871 kb on - strand, within BWI76_RS16345 at 3392.871 kb on - strand, within BWI76_RS16345 at 3392.873 kb on + strand, within BWI76_RS16345 at 3392.883 kb on + strand, within BWI76_RS16345 at 3392.913 kb on + strand, within BWI76_RS16345 at 3392.931 kb on - strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.974 kb on + strand, within BWI76_RS16345 at 3392.980 kb on - strand, within BWI76_RS16345 at 3392.982 kb on - strand, within BWI76_RS16345 at 3392.982 kb on - strand, within BWI76_RS16345 at 3393.006 kb on - strand at 3393.029 kb on + strand at 3393.029 kb on + strand at 3393.037 kb on - strand at 3393.145 kb on + strand, within BWI76_RS16350 at 3393.155 kb on + strand, within BWI76_RS16350 at 3393.158 kb on - strand, within BWI76_RS16350 at 3393.209 kb on + strand, within BWI76_RS16350 at 3393.219 kb on - strand, within BWI76_RS16350 at 3393.243 kb on + strand, within BWI76_RS16350 at 3393.254 kb on + strand, within BWI76_RS16350 at 3393.293 kb on - strand, within BWI76_RS16350 at 3393.295 kb on - strand, within BWI76_RS16350 at 3393.298 kb on - strand, within BWI76_RS16350 at 3393.383 kb on - strand, within BWI76_RS16350 at 3393.533 kb on + strand at 3393.551 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 3,390,199 - BWI76_RS16330 0.45 -1.4 3,390,437 - BWI76_RS16330 0.68 +0.4 3,390,553 - BWI76_RS16330 0.79 -0.3 3,390,856 - +1.1 3,391,018 - BWI76_RS16335 0.49 +0.4 3,391,100 + +2.3 3,391,237 + +0.2 3,391,303 - -0.1 3,391,330 - -0.5 3,392,157 - BWI76_RS16340 0.70 -1.8 3,392,193 + BWI76_RS16340 0.73 -0.3 3,392,214 + BWI76_RS16340 0.74 -0.2 3,392,354 - BWI76_RS16340 0.84 -1.3 3,392,390 + BWI76_RS16340 0.87 -1.3 3,392,393 - BWI76_RS16340 0.87 -2.1 3,392,398 - BWI76_RS16340 0.88 -0.3 3,392,413 - BWI76_RS16340 0.89 -1.1 3,392,414 + BWI76_RS16340 0.89 -1.6 3,392,415 - BWI76_RS16340 0.89 -1.7 3,392,472 + -1.3 3,392,480 - -0.9 3,392,562 + -2.0 3,392,562 + -2.1 3,392,617 - -0.5 3,392,718 + +1.1 3,392,723 + -0.5 3,392,728 + +1.3 3,392,728 + -0.2 3,392,731 - +0.2 3,392,736 + -1.9 3,392,736 - -1.1 3,392,743 + -0.3 3,392,863 + BWI76_RS16345 0.34 +0.6 3,392,871 - BWI76_RS16345 0.38 -0.5 3,392,871 - BWI76_RS16345 0.38 -1.4 3,392,873 + BWI76_RS16345 0.39 +0.5 3,392,883 + BWI76_RS16345 0.44 +1.7 3,392,913 + BWI76_RS16345 0.57 -0.6 3,392,931 - BWI76_RS16345 0.66 +0.5 3,392,974 + BWI76_RS16345 0.86 -2.4 3,392,974 + BWI76_RS16345 0.86 -1.8 3,392,974 + BWI76_RS16345 0.86 +1.5 3,392,974 + BWI76_RS16345 0.86 -2.7 3,392,974 + BWI76_RS16345 0.86 +0.7 3,392,974 + BWI76_RS16345 0.86 +1.7 3,392,974 + BWI76_RS16345 0.86 -0.6 3,392,974 + BWI76_RS16345 0.86 -0.5 3,392,974 + BWI76_RS16345 0.86 +1.4 3,392,974 + BWI76_RS16345 0.86 +4.3 3,392,974 + BWI76_RS16345 0.86 -1.2 3,392,980 - BWI76_RS16345 0.88 -2.5 3,392,982 - BWI76_RS16345 0.89 -0.9 3,392,982 - BWI76_RS16345 0.89 +0.7 3,393,006 - -0.9 3,393,029 + -1.6 3,393,029 + -0.3 3,393,037 - +0.6 3,393,145 + BWI76_RS16350 0.14 +0.5 3,393,155 + BWI76_RS16350 0.16 -1.8 3,393,158 - BWI76_RS16350 0.17 +2.3 3,393,209 + BWI76_RS16350 0.28 -0.2 3,393,219 - BWI76_RS16350 0.31 +0.0 3,393,243 + BWI76_RS16350 0.36 +0.5 3,393,254 + BWI76_RS16350 0.39 +0.2 3,393,293 - BWI76_RS16350 0.47 -1.9 3,393,295 - BWI76_RS16350 0.48 +0.4 3,393,298 - BWI76_RS16350 0.49 -2.2 3,393,383 - BWI76_RS16350 0.68 +0.2 3,393,533 + -3.0 3,393,551 - +0.9
Or see this region's nucleotide sequence