Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15780 and BWI76_RS15785 are separated by 42 nucleotides BWI76_RS15785 and BWI76_RS15790 overlap by 4 nucleotides BWI76_RS15790 and BWI76_RS15795 are separated by 133 nucleotides
BWI76_RS15780: BWI76_RS15780 - hypothetical protein, at 3,284,273 to 3,285,513
_RS15780
BWI76_RS15785: BWI76_RS15785 - replication protein, at 3,285,556 to 3,286,017
_RS15785
BWI76_RS15790: BWI76_RS15790 - replication protein, at 3,286,014 to 3,286,742
_RS15790
BWI76_RS15795: BWI76_RS15795 - bacteriophage CII family protein, at 3,286,876 to 3,287,097
_RS15795
Position (kb) 3285
3286
3287 Strain fitness (log2 ratio) -1
0
1
2 at 3285.556 kb on + strand at 3285.612 kb on + strand, within BWI76_RS15785 at 3285.708 kb on + strand, within BWI76_RS15785 at 3285.816 kb on + strand, within BWI76_RS15785 at 3285.998 kb on + strand at 3286.105 kb on + strand, within BWI76_RS15790 at 3286.245 kb on + strand, within BWI76_RS15790 at 3286.453 kb on - strand, within BWI76_RS15790 at 3286.687 kb on + strand at 3286.709 kb on - strand at 3286.717 kb on - strand at 3286.925 kb on + strand, within BWI76_RS15795 at 3286.974 kb on + strand, within BWI76_RS15795
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 3,285,556 + +2.4 3,285,612 + BWI76_RS15785 0.12 -0.6 3,285,708 + BWI76_RS15785 0.33 -1.0 3,285,816 + BWI76_RS15785 0.56 -1.2 3,285,998 + +0.9 3,286,105 + BWI76_RS15790 0.12 -0.1 3,286,245 + BWI76_RS15790 0.32 +0.6 3,286,453 - BWI76_RS15790 0.60 -0.2 3,286,687 + -0.1 3,286,709 - -0.5 3,286,717 - +0.7 3,286,925 + BWI76_RS15795 0.22 -1.2 3,286,974 + BWI76_RS15795 0.44 -0.3
Or see this region's nucleotide sequence