Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14930

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14920 and BWI76_RS14925 overlap by 4 nucleotidesBWI76_RS14925 and BWI76_RS14930 are separated by 24 nucleotidesBWI76_RS14930 and BWI76_RS14935 are separated by 288 nucleotidesBWI76_RS14935 and BWI76_RS14940 are separated by 139 nucleotides BWI76_RS14920: BWI76_RS14920 - ABC transporter permease, at 3,127,403 to 3,128,410 _RS14920 BWI76_RS14925: BWI76_RS14925 - ABC transporter substrate-binding protein, at 3,128,407 to 3,129,363 _RS14925 BWI76_RS14930: BWI76_RS14930 - hypothetical protein, at 3,129,388 to 3,130,197 _RS14930 BWI76_RS14935: BWI76_RS14935 - NIPSNAP family containing protein, at 3,130,486 to 3,130,815 _RS14935 BWI76_RS14940: BWI76_RS14940 - hypothetical protein, at 3,130,955 to 3,131,803 _RS14940 Position (kb) 3129 3130 3131Strain fitness (log2 ratio) -1 0 1at 3128.672 kb on - strand, within BWI76_RS14925at 3128.743 kb on + strand, within BWI76_RS14925at 3128.910 kb on - strand, within BWI76_RS14925at 3128.915 kb on - strand, within BWI76_RS14925at 3129.255 kb on + strand, within BWI76_RS14925at 3129.288 kb on - strandat 3129.397 kb on + strandat 3129.607 kb on + strand, within BWI76_RS14930at 3130.446 kb on - strandat 3130.734 kb on - strand, within BWI76_RS14935at 3130.933 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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3,128,672 - BWI76_RS14925 0.28 -1.1
3,128,743 + BWI76_RS14925 0.35 +1.2
3,128,910 - BWI76_RS14925 0.53 -0.1
3,128,915 - BWI76_RS14925 0.53 -1.4
3,129,255 + BWI76_RS14925 0.89 -1.1
3,129,288 - +0.8
3,129,397 + -0.6
3,129,607 + BWI76_RS14930 0.27 -1.3
3,130,446 - +0.1
3,130,734 - BWI76_RS14935 0.75 -1.0
3,130,933 + +1.0

Or see this region's nucleotide sequence