Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14125 and BWI76_RS14130 are separated by 247 nucleotides BWI76_RS14130 and BWI76_RS14135 overlap by 4 nucleotides
BWI76_RS14125: BWI76_RS14125 - SDR family oxidoreductase, at 2,951,027 to 2,951,911
_RS14125
BWI76_RS14130: BWI76_RS14130 - malto-oligosyltrehalose trehalohydrolase, at 2,952,159 to 2,953,946
_RS14130
BWI76_RS14135: BWI76_RS14135 - malto-oligosyltrehalose synthase, at 2,953,943 to 2,956,483
_RS14135
Position (kb)
2952
2953
2954 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2951.201 kb on + strand, within BWI76_RS14125 at 2951.201 kb on + strand, within BWI76_RS14125 at 2951.366 kb on + strand, within BWI76_RS14125 at 2951.379 kb on + strand, within BWI76_RS14125 at 2951.457 kb on - strand, within BWI76_RS14125 at 2951.515 kb on + strand, within BWI76_RS14125 at 2951.525 kb on - strand, within BWI76_RS14125 at 2951.622 kb on + strand, within BWI76_RS14125 at 2951.624 kb on - strand, within BWI76_RS14125 at 2951.630 kb on - strand, within BWI76_RS14125 at 2951.630 kb on - strand, within BWI76_RS14125 at 2951.630 kb on - strand, within BWI76_RS14125 at 2951.630 kb on - strand, within BWI76_RS14125 at 2951.650 kb on + strand, within BWI76_RS14125 at 2951.687 kb on + strand, within BWI76_RS14125 at 2951.711 kb on + strand, within BWI76_RS14125 at 2951.716 kb on - strand, within BWI76_RS14125 at 2951.796 kb on + strand, within BWI76_RS14125 at 2951.836 kb on + strand at 2951.857 kb on + strand at 2951.874 kb on + strand at 2951.877 kb on + strand at 2951.882 kb on + strand at 2951.882 kb on + strand at 2951.882 kb on + strand at 2951.882 kb on + strand at 2951.882 kb on + strand at 2951.882 kb on + strand at 2951.885 kb on - strand at 2951.890 kb on - strand at 2951.890 kb on - strand at 2952.071 kb on + strand at 2952.079 kb on - strand at 2952.080 kb on + strand at 2952.083 kb on - strand at 2952.096 kb on - strand at 2952.098 kb on + strand at 2952.103 kb on + strand at 2952.107 kb on - strand at 2952.111 kb on - strand at 2952.206 kb on + strand at 2952.206 kb on + strand at 2952.210 kb on + strand at 2952.240 kb on + strand at 2952.240 kb on + strand at 2952.241 kb on + strand at 2952.250 kb on + strand at 2952.262 kb on + strand at 2952.262 kb on + strand at 2952.270 kb on - strand at 2952.297 kb on + strand at 2952.297 kb on + strand at 2952.305 kb on - strand at 2952.305 kb on - strand at 2952.305 kb on - strand at 2952.305 kb on - strand at 2952.305 kb on - strand at 2952.305 kb on - strand at 2952.327 kb on + strand at 2952.419 kb on - strand, within BWI76_RS14130 at 2952.421 kb on - strand, within BWI76_RS14130 at 2952.424 kb on + strand, within BWI76_RS14130 at 2952.424 kb on + strand, within BWI76_RS14130 at 2952.430 kb on - strand, within BWI76_RS14130 at 2952.618 kb on - strand, within BWI76_RS14130 at 2952.706 kb on + strand, within BWI76_RS14130 at 2952.727 kb on + strand, within BWI76_RS14130 at 2952.728 kb on - strand, within BWI76_RS14130 at 2952.728 kb on - strand, within BWI76_RS14130 at 2952.785 kb on - strand, within BWI76_RS14130 at 2952.842 kb on - strand, within BWI76_RS14130 at 2952.886 kb on - strand, within BWI76_RS14130 at 2953.047 kb on - strand, within BWI76_RS14130 at 2953.111 kb on - strand, within BWI76_RS14130 at 2953.445 kb on + strand, within BWI76_RS14130 at 2953.453 kb on - strand at 2953.906 kb on + strand at 2954.351 kb on + strand, within BWI76_RS14135 at 2954.433 kb on - strand, within BWI76_RS14135
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 2,951,201 + BWI76_RS14125 0.20 +0.2 2,951,201 + BWI76_RS14125 0.20 -1.7 2,951,366 + BWI76_RS14125 0.38 -0.8 2,951,379 + BWI76_RS14125 0.40 +1.1 2,951,457 - BWI76_RS14125 0.49 -1.2 2,951,515 + BWI76_RS14125 0.55 -0.1 2,951,525 - BWI76_RS14125 0.56 +0.3 2,951,622 + BWI76_RS14125 0.67 -0.6 2,951,624 - BWI76_RS14125 0.67 -0.2 2,951,630 - BWI76_RS14125 0.68 +0.2 2,951,630 - BWI76_RS14125 0.68 +1.0 2,951,630 - BWI76_RS14125 0.68 +1.1 2,951,630 - BWI76_RS14125 0.68 +1.2 2,951,650 + BWI76_RS14125 0.70 -1.7 2,951,687 + BWI76_RS14125 0.75 -2.7 2,951,711 + BWI76_RS14125 0.77 -0.4 2,951,716 - BWI76_RS14125 0.78 +0.7 2,951,796 + BWI76_RS14125 0.87 -1.0 2,951,836 + +2.2 2,951,857 + -1.1 2,951,874 + +0.8 2,951,877 + +0.2 2,951,882 + -0.9 2,951,882 + -0.1 2,951,882 + +0.7 2,951,882 + +0.4 2,951,882 + +0.0 2,951,882 + +0.9 2,951,885 - +0.2 2,951,890 - +1.9 2,951,890 - -0.6 2,952,071 + +2.1 2,952,079 - -2.1 2,952,080 + -0.4 2,952,083 - -1.2 2,952,096 - +0.4 2,952,098 + -0.5 2,952,103 + -0.8 2,952,107 - -0.4 2,952,111 - +1.4 2,952,206 + -0.2 2,952,206 + +0.2 2,952,210 + -0.3 2,952,240 + -0.4 2,952,240 + -1.6 2,952,241 + -3.0 2,952,250 + -3.0 2,952,262 + +1.4 2,952,262 + +0.0 2,952,270 - +2.2 2,952,297 + -3.1 2,952,297 + -1.1 2,952,305 - +1.2 2,952,305 - -3.0 2,952,305 - +0.6 2,952,305 - +1.6 2,952,305 - +2.3 2,952,305 - -0.1 2,952,327 + +2.9 2,952,419 - BWI76_RS14130 0.15 -1.9 2,952,421 - BWI76_RS14130 0.15 +1.5 2,952,424 + BWI76_RS14130 0.15 -0.1 2,952,424 + BWI76_RS14130 0.15 +0.8 2,952,430 - BWI76_RS14130 0.15 -2.3 2,952,618 - BWI76_RS14130 0.26 -1.4 2,952,706 + BWI76_RS14130 0.31 -0.1 2,952,727 + BWI76_RS14130 0.32 -3.8 2,952,728 - BWI76_RS14130 0.32 -1.7 2,952,728 - BWI76_RS14130 0.32 -2.6 2,952,785 - BWI76_RS14130 0.35 +0.6 2,952,842 - BWI76_RS14130 0.38 +0.7 2,952,886 - BWI76_RS14130 0.41 -1.0 2,953,047 - BWI76_RS14130 0.50 -1.3 2,953,111 - BWI76_RS14130 0.53 -3.6 2,953,445 + BWI76_RS14130 0.72 -2.7 2,953,453 - +2.6 2,953,906 + +0.7 2,954,351 + BWI76_RS14135 0.16 +0.8 2,954,433 - BWI76_RS14135 0.19 -0.6
Or see this region's nucleotide sequence