Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12865

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotidesBWI76_RS12860 and BWI76_RS12865 overlap by 8 nucleotidesBWI76_RS12865 and BWI76_RS12870 are separated by 23 nucleotidesBWI76_RS12870 and BWI76_RS12875 are separated by 159 nucleotides BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189 _RS12855 BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860 _RS12860 BWI76_RS12865: BWI76_RS12865 - acetylornithine deacetylase, at 2,680,853 to 2,682,022 _RS12865 BWI76_RS12870: BWI76_RS12870 - putative phytanoyl-CoA dioxygenase, at 2,682,046 to 2,682,861 _RS12870 BWI76_RS12875: BWI76_RS12875 - bifunctional deaminase-reductase, at 2,683,021 to 2,683,557 _RS12875 Position (kb) 2680 2681 2682 2683Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2680.044 kb on + strand, within BWI76_RS12855at 2680.303 kb on + strand, within BWI76_RS12860at 2680.518 kb on - strand, within BWI76_RS12860at 2681.019 kb on - strand, within BWI76_RS12865at 2681.110 kb on - strand, within BWI76_RS12865at 2681.268 kb on + strand, within BWI76_RS12865at 2681.368 kb on - strand, within BWI76_RS12865at 2681.368 kb on - strand, within BWI76_RS12865at 2681.518 kb on - strand, within BWI76_RS12865at 2681.906 kb on + strandat 2681.921 kb on - strandat 2682.117 kb on + strandat 2682.117 kb on + strandat 2682.205 kb on - strand, within BWI76_RS12870at 2682.302 kb on + strand, within BWI76_RS12870at 2682.418 kb on + strand, within BWI76_RS12870at 2682.797 kb on + strandat 2682.911 kb on + strandat 2683.019 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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2,680,044 + BWI76_RS12855 0.66 -3.1
2,680,303 + BWI76_RS12860 0.17 -0.8
2,680,518 - BWI76_RS12860 0.49 -0.7
2,681,019 - BWI76_RS12865 0.14 -1.8
2,681,110 - BWI76_RS12865 0.22 -1.3
2,681,268 + BWI76_RS12865 0.35 -0.1
2,681,368 - BWI76_RS12865 0.44 -0.1
2,681,368 - BWI76_RS12865 0.44 -1.9
2,681,518 - BWI76_RS12865 0.57 -0.2
2,681,906 + +1.9
2,681,921 - -0.9
2,682,117 + -0.7
2,682,117 + +0.0
2,682,205 - BWI76_RS12870 0.19 +0.9
2,682,302 + BWI76_RS12870 0.31 +0.3
2,682,418 + BWI76_RS12870 0.46 +0.6
2,682,797 + +0.3
2,682,911 + -1.3
2,683,019 + +0.1

Or see this region's nucleotide sequence