Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12325 and BWI76_RS12330 overlap by 1 nucleotides BWI76_RS12330 and BWI76_RS12335 overlap by 14 nucleotides BWI76_RS12335 and BWI76_RS12340 are separated by 224 nucleotides
BWI76_RS12325: BWI76_RS12325 - hypothetical protein, at 2,570,941 to 2,574,228
_RS12325
BWI76_RS12330: BWI76_RS12330 - hypothetical protein, at 2,574,228 to 2,574,701
_RS12330
BWI76_RS12335: BWI76_RS12335 - hypothetical protein, at 2,574,688 to 2,575,170
_RS12335
BWI76_RS12340: BWI76_RS12340 - molecular chaperone Tir, at 2,575,395 to 2,576,276
_RS12340
Position (kb)
2574
2575 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2573.278 kb on + strand at 2573.306 kb on + strand, within BWI76_RS12325 at 2573.333 kb on - strand, within BWI76_RS12325 at 2573.367 kb on + strand, within BWI76_RS12325 at 2573.374 kb on - strand, within BWI76_RS12325 at 2573.379 kb on - strand, within BWI76_RS12325 at 2573.384 kb on + strand, within BWI76_RS12325 at 2573.394 kb on + strand, within BWI76_RS12325 at 2573.461 kb on - strand, within BWI76_RS12325 at 2573.496 kb on - strand, within BWI76_RS12325 at 2573.522 kb on + strand, within BWI76_RS12325 at 2573.536 kb on - strand, within BWI76_RS12325 at 2573.630 kb on + strand, within BWI76_RS12325 at 2573.654 kb on - strand at 2573.900 kb on - strand at 2574.018 kb on - strand at 2574.080 kb on + strand at 2574.081 kb on - strand at 2574.088 kb on - strand at 2574.088 kb on - strand at 2574.131 kb on - strand at 2574.145 kb on - strand at 2574.160 kb on + strand at 2574.247 kb on - strand at 2574.366 kb on - strand, within BWI76_RS12330 at 2574.386 kb on - strand, within BWI76_RS12330 at 2574.459 kb on + strand, within BWI76_RS12330 at 2574.668 kb on + strand at 2574.668 kb on + strand at 2574.675 kb on - strand at 2574.722 kb on + strand at 2574.827 kb on + strand, within BWI76_RS12335 at 2574.835 kb on - strand, within BWI76_RS12335 at 2574.843 kb on - strand, within BWI76_RS12335 at 2574.862 kb on - strand, within BWI76_RS12335 at 2575.011 kb on - strand at 2575.034 kb on - strand, within BWI76_RS12335 at 2575.034 kb on - strand, within BWI76_RS12335 at 2575.075 kb on - strand, within BWI76_RS12335 at 2575.087 kb on + strand, within BWI76_RS12335 at 2575.087 kb on + strand, within BWI76_RS12335 at 2575.091 kb on + strand, within BWI76_RS12335 at 2575.091 kb on + strand, within BWI76_RS12335 at 2575.095 kb on - strand, within BWI76_RS12335 at 2575.095 kb on - strand, within BWI76_RS12335 at 2575.099 kb on - strand, within BWI76_RS12335 at 2575.099 kb on - strand, within BWI76_RS12335 at 2575.099 kb on - strand, within BWI76_RS12335 at 2575.099 kb on - strand, within BWI76_RS12335 at 2575.115 kb on + strand, within BWI76_RS12335 at 2575.119 kb on - strand, within BWI76_RS12335 at 2575.125 kb on - strand at 2575.179 kb on + strand at 2575.186 kb on - strand at 2575.312 kb on + strand at 2575.322 kb on + strand at 2575.322 kb on + strand at 2575.322 kb on + strand at 2575.329 kb on + strand at 2575.329 kb on + strand at 2575.329 kb on + strand at 2575.329 kb on + strand at 2575.335 kb on + strand at 2575.335 kb on + strand at 2575.337 kb on - strand at 2575.355 kb on - strand at 2575.416 kb on - strand at 2575.424 kb on - strand at 2575.464 kb on - strand at 2575.585 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 2,573,278 + +0.7 2,573,306 + BWI76_RS12325 0.72 +0.8 2,573,333 - BWI76_RS12325 0.73 -2.9 2,573,367 + BWI76_RS12325 0.74 +0.1 2,573,374 - BWI76_RS12325 0.74 -0.4 2,573,379 - BWI76_RS12325 0.74 -0.2 2,573,384 + BWI76_RS12325 0.74 +1.0 2,573,394 + BWI76_RS12325 0.75 -0.1 2,573,461 - BWI76_RS12325 0.77 -1.1 2,573,496 - BWI76_RS12325 0.78 -1.1 2,573,522 + BWI76_RS12325 0.78 +0.5 2,573,536 - BWI76_RS12325 0.79 +0.1 2,573,630 + BWI76_RS12325 0.82 +0.7 2,573,654 - +0.7 2,573,900 - -1.9 2,574,018 - -1.3 2,574,080 + +0.1 2,574,081 - +0.6 2,574,088 - +0.4 2,574,088 - -2.2 2,574,131 - -0.3 2,574,145 - -0.9 2,574,160 + +1.5 2,574,247 - +1.3 2,574,366 - BWI76_RS12330 0.29 -1.0 2,574,386 - BWI76_RS12330 0.33 -1.8 2,574,459 + BWI76_RS12330 0.49 -1.3 2,574,668 + -0.0 2,574,668 + +0.0 2,574,675 - -0.7 2,574,722 + -0.8 2,574,827 + BWI76_RS12335 0.29 -2.4 2,574,835 - BWI76_RS12335 0.30 +4.5 2,574,843 - BWI76_RS12335 0.32 +0.6 2,574,862 - BWI76_RS12335 0.36 +2.3 2,575,011 - +1.3 2,575,034 - BWI76_RS12335 0.72 -2.6 2,575,034 - BWI76_RS12335 0.72 -0.3 2,575,075 - BWI76_RS12335 0.80 -0.6 2,575,087 + BWI76_RS12335 0.83 +2.3 2,575,087 + BWI76_RS12335 0.83 +0.3 2,575,091 + BWI76_RS12335 0.83 +2.3 2,575,091 + BWI76_RS12335 0.83 -0.3 2,575,095 - BWI76_RS12335 0.84 -1.6 2,575,095 - BWI76_RS12335 0.84 +0.8 2,575,099 - BWI76_RS12335 0.85 -0.3 2,575,099 - BWI76_RS12335 0.85 -1.4 2,575,099 - BWI76_RS12335 0.85 +0.3 2,575,099 - BWI76_RS12335 0.85 +1.3 2,575,115 + BWI76_RS12335 0.88 +2.3 2,575,119 - BWI76_RS12335 0.89 +2.3 2,575,125 - -1.4 2,575,179 + -1.4 2,575,186 - -1.3 2,575,312 + -2.0 2,575,322 + +2.3 2,575,322 + -0.2 2,575,322 + +0.3 2,575,329 + -2.0 2,575,329 + -2.5 2,575,329 + +2.5 2,575,329 + +0.8 2,575,335 + +2.3 2,575,335 + -0.3 2,575,337 - +1.3 2,575,355 - -0.4 2,575,416 - +3.8 2,575,424 - -0.3 2,575,464 - +0.3 2,575,585 + +1.3
Or see this region's nucleotide sequence