Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10750 and BWI76_RS10755 are separated by 21 nucleotides BWI76_RS10755 and BWI76_RS10760 are separated by 9 nucleotides BWI76_RS10760 and BWI76_RS10765 overlap by 4 nucleotides
BWI76_RS10750: BWI76_RS10750 - pyrimidine utilization transport protein G, at 2,270,508 to 2,271,830
_RS10750
BWI76_RS10755: BWI76_RS10755 - pyrimidine utilization flavin reductase protein F, at 2,271,852 to 2,272,346
_RS10755
BWI76_RS10760: BWI76_RS10760 - nitroreductase family protein, at 2,272,356 to 2,272,946
_RS10760
BWI76_RS10765: BWI76_RS10765 - pyrimidine utilization protein D, at 2,272,943 to 2,273,746
_RS10765
Position (kb)
2271
2272
2273 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2270.872 kb on - strand, within BWI76_RS10750 at 2271.104 kb on - strand, within BWI76_RS10750 at 2271.359 kb on + strand, within BWI76_RS10750 at 2271.479 kb on + strand, within BWI76_RS10750 at 2271.499 kb on + strand, within BWI76_RS10750 at 2271.507 kb on - strand, within BWI76_RS10750 at 2271.852 kb on - strand at 2271.911 kb on + strand, within BWI76_RS10755 at 2272.362 kb on - strand at 2272.685 kb on - strand, within BWI76_RS10760 at 2272.734 kb on + strand, within BWI76_RS10760 at 2272.741 kb on + strand, within BWI76_RS10760 at 2272.742 kb on - strand at 2273.158 kb on - strand, within BWI76_RS10765 at 2273.158 kb on - strand, within BWI76_RS10765
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 2,270,872 - BWI76_RS10750 0.28 -0.6 2,271,104 - BWI76_RS10750 0.45 -0.3 2,271,359 + BWI76_RS10750 0.64 -0.1 2,271,479 + BWI76_RS10750 0.73 +1.1 2,271,499 + BWI76_RS10750 0.75 +0.2 2,271,507 - BWI76_RS10750 0.76 -1.1 2,271,852 - +0.3 2,271,911 + BWI76_RS10755 0.12 -3.8 2,272,362 - +0.6 2,272,685 - BWI76_RS10760 0.56 +0.8 2,272,734 + BWI76_RS10760 0.64 -0.5 2,272,741 + BWI76_RS10760 0.65 +0.6 2,272,742 - +1.2 2,273,158 - BWI76_RS10765 0.27 +0.2 2,273,158 - BWI76_RS10765 0.27 -0.3
Or see this region's nucleotide sequence