Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10755

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10750 and BWI76_RS10755 are separated by 21 nucleotidesBWI76_RS10755 and BWI76_RS10760 are separated by 9 nucleotidesBWI76_RS10760 and BWI76_RS10765 overlap by 4 nucleotides BWI76_RS10750: BWI76_RS10750 - pyrimidine utilization transport protein G, at 2,270,508 to 2,271,830 _RS10750 BWI76_RS10755: BWI76_RS10755 - pyrimidine utilization flavin reductase protein F, at 2,271,852 to 2,272,346 _RS10755 BWI76_RS10760: BWI76_RS10760 - nitroreductase family protein, at 2,272,356 to 2,272,946 _RS10760 BWI76_RS10765: BWI76_RS10765 - pyrimidine utilization protein D, at 2,272,943 to 2,273,746 _RS10765 Position (kb) 2271 2272 2273Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2270.872 kb on - strand, within BWI76_RS10750at 2271.104 kb on - strand, within BWI76_RS10750at 2271.359 kb on + strand, within BWI76_RS10750at 2271.479 kb on + strand, within BWI76_RS10750at 2271.499 kb on + strand, within BWI76_RS10750at 2271.507 kb on - strand, within BWI76_RS10750at 2271.852 kb on - strandat 2271.911 kb on + strand, within BWI76_RS10755at 2272.362 kb on - strandat 2272.685 kb on - strand, within BWI76_RS10760at 2272.734 kb on + strand, within BWI76_RS10760at 2272.741 kb on + strand, within BWI76_RS10760at 2272.742 kb on - strandat 2273.158 kb on - strand, within BWI76_RS10765at 2273.158 kb on - strand, within BWI76_RS10765

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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2,270,872 - BWI76_RS10750 0.28 -0.6
2,271,104 - BWI76_RS10750 0.45 -0.3
2,271,359 + BWI76_RS10750 0.64 -0.1
2,271,479 + BWI76_RS10750 0.73 +1.1
2,271,499 + BWI76_RS10750 0.75 +0.2
2,271,507 - BWI76_RS10750 0.76 -1.1
2,271,852 - +0.3
2,271,911 + BWI76_RS10755 0.12 -3.8
2,272,362 - +0.6
2,272,685 - BWI76_RS10760 0.56 +0.8
2,272,734 + BWI76_RS10760 0.64 -0.5
2,272,741 + BWI76_RS10760 0.65 +0.6
2,272,742 - +1.2
2,273,158 - BWI76_RS10765 0.27 +0.2
2,273,158 - BWI76_RS10765 0.27 -0.3

Or see this region's nucleotide sequence