Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09890 and BWI76_RS09895 are separated by 66 nucleotides BWI76_RS09895 and BWI76_RS09900 overlap by 4 nucleotides BWI76_RS09900 and BWI76_RS09905 are separated by 56 nucleotides
BWI76_RS09890: BWI76_RS09890 - aminopeptidase N, at 2,079,105 to 2,081,720
_RS09890
BWI76_RS09895: BWI76_RS09895 - aliphatic sulfonates ABC transporter ATP-binding protein, at 2,081,787 to 2,082,560
_RS09895
BWI76_RS09900: BWI76_RS09900 - alkanesulfonate transporter permease subunit, at 2,082,557 to 2,083,348
_RS09900
BWI76_RS09905: BWI76_RS09905 - FMN reductase (NADPH), at 2,083,405 to 2,083,980
_RS09905
Position (kb)
2081
2082
2083 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2081.051 kb on + strand, within BWI76_RS09890 at 2081.126 kb on - strand, within BWI76_RS09890 at 2081.147 kb on + strand, within BWI76_RS09890 at 2081.175 kb on - strand, within BWI76_RS09890 at 2081.224 kb on - strand, within BWI76_RS09890 at 2081.510 kb on + strand at 2081.555 kb on - strand at 2081.584 kb on + strand at 2081.723 kb on - strand at 2081.834 kb on + strand at 2082.191 kb on + strand, within BWI76_RS09895 at 2082.191 kb on + strand, within BWI76_RS09895 at 2082.522 kb on - strand at 2082.679 kb on + strand, within BWI76_RS09900 at 2082.746 kb on + strand, within BWI76_RS09900 at 2082.824 kb on + strand, within BWI76_RS09900 at 2082.824 kb on + strand, within BWI76_RS09900 at 2083.022 kb on + strand, within BWI76_RS09900 at 2083.086 kb on + strand, within BWI76_RS09900 at 2083.155 kb on - strand, within BWI76_RS09900 at 2083.381 kb on + strand at 2083.462 kb on + strand at 2083.470 kb on - strand, within BWI76_RS09905
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 2,081,051 + BWI76_RS09890 0.74 +0.3 2,081,126 - BWI76_RS09890 0.77 -1.6 2,081,147 + BWI76_RS09890 0.78 -0.4 2,081,175 - BWI76_RS09890 0.79 +0.3 2,081,224 - BWI76_RS09890 0.81 +0.1 2,081,510 + -0.9 2,081,555 - -0.7 2,081,584 + -3.3 2,081,723 - -2.5 2,081,834 + -0.2 2,082,191 + BWI76_RS09895 0.52 +0.4 2,082,191 + BWI76_RS09895 0.52 +0.2 2,082,522 - -2.2 2,082,679 + BWI76_RS09900 0.15 -0.4 2,082,746 + BWI76_RS09900 0.24 -0.9 2,082,824 + BWI76_RS09900 0.34 -0.8 2,082,824 + BWI76_RS09900 0.34 -1.3 2,083,022 + BWI76_RS09900 0.59 -0.7 2,083,086 + BWI76_RS09900 0.67 -0.7 2,083,155 - BWI76_RS09900 0.76 +2.1 2,083,381 + +1.0 2,083,462 + -0.2 2,083,470 - BWI76_RS09905 0.11 +0.0
Or see this region's nucleotide sequence