Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07450 and BWI76_RS07455 are separated by 265 nucleotides BWI76_RS07455 and BWI76_RS07460 are separated by 118 nucleotides BWI76_RS07460 and BWI76_RS07465 overlap by 1 nucleotides
BWI76_RS07450: BWI76_RS07450 - transcriptional regulator, at 1,572,658 to 1,573,524
_RS07450
BWI76_RS07455: BWI76_RS07455 - NAD(P)H nitroreductase, at 1,573,790 to 1,574,443
_RS07455
BWI76_RS07460: BWI76_RS07460 - hypothetical protein, at 1,574,562 to 1,574,930
_RS07460
BWI76_RS07465: BWI76_RS07465 - TetR family transcriptional regulator, at 1,574,930 to 1,575,511
_RS07465
Position (kb)
1573
1574
1575 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1572.924 kb on + strand, within BWI76_RS07450 at 1573.171 kb on - strand, within BWI76_RS07450 at 1573.321 kb on + strand, within BWI76_RS07450 at 1573.332 kb on - strand, within BWI76_RS07450 at 1573.344 kb on + strand, within BWI76_RS07450 at 1573.430 kb on - strand, within BWI76_RS07450 at 1573.704 kb on + strand at 1573.705 kb on - strand at 1573.714 kb on + strand at 1573.827 kb on + strand at 1573.882 kb on + strand, within BWI76_RS07455 at 1573.901 kb on - strand, within BWI76_RS07455 at 1573.906 kb on - strand, within BWI76_RS07455 at 1574.160 kb on + strand, within BWI76_RS07455 at 1574.165 kb on + strand, within BWI76_RS07455 at 1574.465 kb on - strand at 1574.702 kb on + strand, within BWI76_RS07460 at 1574.900 kb on + strand at 1574.947 kb on - strand at 1575.250 kb on - strand, within BWI76_RS07465 at 1575.306 kb on + strand, within BWI76_RS07465
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 1,572,924 + BWI76_RS07450 0.31 +0.3 1,573,171 - BWI76_RS07450 0.59 +0.7 1,573,321 + BWI76_RS07450 0.76 +1.3 1,573,332 - BWI76_RS07450 0.78 +1.1 1,573,344 + BWI76_RS07450 0.79 -1.8 1,573,430 - BWI76_RS07450 0.89 -1.0 1,573,704 + -0.7 1,573,705 - +0.4 1,573,714 + +2.9 1,573,827 + -0.4 1,573,882 + BWI76_RS07455 0.14 -1.1 1,573,901 - BWI76_RS07455 0.17 +0.4 1,573,906 - BWI76_RS07455 0.18 +0.1 1,574,160 + BWI76_RS07455 0.57 -1.8 1,574,165 + BWI76_RS07455 0.57 -1.7 1,574,465 - -0.4 1,574,702 + BWI76_RS07460 0.38 -0.1 1,574,900 + -1.6 1,574,947 - +1.0 1,575,250 - BWI76_RS07465 0.55 +1.1 1,575,306 + BWI76_RS07465 0.65 -1.6
Or see this region's nucleotide sequence