Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06830

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06820 and BWI76_RS06825 are separated by 57 nucleotidesBWI76_RS06825 and BWI76_RS06830 overlap by 1 nucleotidesBWI76_RS06830 and BWI76_RS06835 are separated by 111 nucleotides BWI76_RS06820: BWI76_RS06820 - DNA polymerase III subunit gamma/tau, at 1,430,090 to 1,431,991 _RS06820 BWI76_RS06825: BWI76_RS06825 - nucleoid-associated protein, YbaB/EbfC family, at 1,432,049 to 1,432,381 _RS06825 BWI76_RS06830: BWI76_RS06830 - recombination protein RecR, at 1,432,381 to 1,432,986 _RS06830 BWI76_RS06835: BWI76_RS06835 - molecular chaperone HtpG, at 1,433,098 to 1,434,972 _RS06835 Position (kb) 1432 1433Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1432.436 kb on + strandat 1432.636 kb on + strand, within BWI76_RS06830at 1432.694 kb on + strand, within BWI76_RS06830at 1432.869 kb on + strand, within BWI76_RS06830at 1432.874 kb on + strand, within BWI76_RS06830at 1433.024 kb on + strandat 1433.035 kb on + strandat 1433.213 kb on + strandat 1433.347 kb on + strand, within BWI76_RS06835at 1433.436 kb on + strand, within BWI76_RS06835at 1433.436 kb on + strand, within BWI76_RS06835at 1433.535 kb on - strand, within BWI76_RS06835at 1433.914 kb on + strand, within BWI76_RS06835at 1433.928 kb on + strand, within BWI76_RS06835

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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1,432,436 + -0.7
1,432,636 + BWI76_RS06830 0.42 -0.3
1,432,694 + BWI76_RS06830 0.52 -1.3
1,432,869 + BWI76_RS06830 0.81 -3.9
1,432,874 + BWI76_RS06830 0.81 -3.0
1,433,024 + -1.0
1,433,035 + -0.7
1,433,213 + -0.3
1,433,347 + BWI76_RS06835 0.13 -0.1
1,433,436 + BWI76_RS06835 0.18 -0.8
1,433,436 + BWI76_RS06835 0.18 +1.1
1,433,535 - BWI76_RS06835 0.23 -0.2
1,433,914 + BWI76_RS06835 0.44 -0.7
1,433,928 + BWI76_RS06835 0.44 -0.3

Or see this region's nucleotide sequence