Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06575 and BWI76_RS06580 are separated by 115 nucleotides BWI76_RS06580 and BWI76_RS06585 are separated by 42 nucleotides BWI76_RS06585 and BWI76_RS06590 overlap by 8 nucleotides
BWI76_RS06575: BWI76_RS06575 - cysteine synthase, at 1,382,307 to 1,383,359
_RS06575
BWI76_RS06580: BWI76_RS06580 - transcriptional regulator, at 1,383,475 to 1,383,933
_RS06580
BWI76_RS06585: BWI76_RS06585 - multidrug ABC transporter permease/ATP-binding protein, at 1,383,976 to 1,385,748
_RS06585
BWI76_RS06590: BWI76_RS06590 - multidrug ABC transporter permease/ATP-binding protein, at 1,385,741 to 1,387,519
_RS06590
Position (kb)
1383
1384
1385
1386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1383.246 kb on + strand, within BWI76_RS06575 at 1383.398 kb on - strand at 1383.562 kb on - strand, within BWI76_RS06580 at 1383.567 kb on - strand, within BWI76_RS06580 at 1383.689 kb on - strand, within BWI76_RS06580 at 1383.964 kb on + strand at 1384.045 kb on - strand at 1384.241 kb on + strand, within BWI76_RS06585 at 1384.397 kb on + strand, within BWI76_RS06585 at 1384.402 kb on + strand, within BWI76_RS06585 at 1384.410 kb on - strand, within BWI76_RS06585 at 1384.410 kb on - strand, within BWI76_RS06585 at 1384.736 kb on + strand, within BWI76_RS06585 at 1384.774 kb on - strand, within BWI76_RS06585 at 1384.871 kb on + strand, within BWI76_RS06585 at 1385.137 kb on - strand, within BWI76_RS06585 at 1385.402 kb on + strand, within BWI76_RS06585 at 1385.526 kb on + strand, within BWI76_RS06585 at 1385.620 kb on + strand at 1385.860 kb on - strand at 1385.945 kb on - strand at 1385.947 kb on + strand, within BWI76_RS06590 at 1385.983 kb on + strand, within BWI76_RS06590 at 1386.152 kb on - strand, within BWI76_RS06590 at 1386.370 kb on + strand, within BWI76_RS06590 at 1386.572 kb on - strand, within BWI76_RS06590
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 1,383,246 + BWI76_RS06575 0.89 +1.3 1,383,398 - +1.3 1,383,562 - BWI76_RS06580 0.19 -1.4 1,383,567 - BWI76_RS06580 0.20 +3.2 1,383,689 - BWI76_RS06580 0.47 +1.1 1,383,964 + -0.5 1,384,045 - -0.1 1,384,241 + BWI76_RS06585 0.15 -0.6 1,384,397 + BWI76_RS06585 0.24 +0.5 1,384,402 + BWI76_RS06585 0.24 -0.1 1,384,410 - BWI76_RS06585 0.24 +3.7 1,384,410 - BWI76_RS06585 0.24 +1.4 1,384,736 + BWI76_RS06585 0.43 -3.7 1,384,774 - BWI76_RS06585 0.45 -0.8 1,384,871 + BWI76_RS06585 0.50 -0.6 1,385,137 - BWI76_RS06585 0.65 -3.3 1,385,402 + BWI76_RS06585 0.80 +0.4 1,385,526 + BWI76_RS06585 0.87 +1.0 1,385,620 + +0.8 1,385,860 - +0.1 1,385,945 - +0.1 1,385,947 + BWI76_RS06590 0.12 -0.1 1,385,983 + BWI76_RS06590 0.14 -1.3 1,386,152 - BWI76_RS06590 0.23 +1.1 1,386,370 + BWI76_RS06590 0.35 -0.6 1,386,572 - BWI76_RS06590 0.47 +0.4
Or see this region's nucleotide sequence