Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06105 and BWI76_RS06110 are separated by 18 nucleotides BWI76_RS06110 and BWI76_RS06115 overlap by 4 nucleotides BWI76_RS06115 and BWI76_RS06120 are separated by 255 nucleotides BWI76_RS06120 and BWI76_RS06125 are separated by 151 nucleotides
BWI76_RS06105: BWI76_RS06105 - microcin B17 transporter, at 1,283,610 to 1,284,830
_RS06105
BWI76_RS06110: BWI76_RS06110 - hypothetical protein, at 1,284,849 to 1,285,955
_RS06110
BWI76_RS06115: BWI76_RS06115 - membrane protein, at 1,285,952 to 1,286,254
_RS06115
BWI76_RS06120: BWI76_RS06120 - membrane protein, at 1,286,510 to 1,286,713
_RS06120
BWI76_RS06125: BWI76_RS06125 - D-alanine--D-alanine ligase A, at 1,286,865 to 1,287,962
_RS06125
Position (kb) 1284
1285
1286 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1283.963 kb on + strand, within BWI76_RS06105 at 1284.006 kb on - strand, within BWI76_RS06105 at 1284.006 kb on - strand, within BWI76_RS06105 at 1284.064 kb on - strand, within BWI76_RS06105 at 1284.210 kb on + strand, within BWI76_RS06105 at 1284.389 kb on + strand, within BWI76_RS06105 at 1284.402 kb on - strand, within BWI76_RS06105 at 1284.461 kb on - strand, within BWI76_RS06105 at 1284.466 kb on - strand, within BWI76_RS06105 at 1284.510 kb on - strand, within BWI76_RS06105 at 1284.512 kb on + strand, within BWI76_RS06105 at 1284.660 kb on - strand, within BWI76_RS06105 at 1284.722 kb on + strand at 1284.780 kb on - strand at 1284.793 kb on + strand at 1284.840 kb on + strand at 1285.266 kb on + strand, within BWI76_RS06110 at 1285.266 kb on + strand, within BWI76_RS06110 at 1285.266 kb on + strand, within BWI76_RS06110 at 1285.266 kb on + strand, within BWI76_RS06110 at 1285.274 kb on - strand, within BWI76_RS06110 at 1285.482 kb on + strand, within BWI76_RS06110 at 1285.944 kb on - strand at 1286.548 kb on - strand, within BWI76_RS06120 at 1286.553 kb on - strand, within BWI76_RS06120 at 1286.766 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 1,283,963 + BWI76_RS06105 0.29 -1.2 1,284,006 - BWI76_RS06105 0.32 +0.3 1,284,006 - BWI76_RS06105 0.32 +0.0 1,284,064 - BWI76_RS06105 0.37 -0.5 1,284,210 + BWI76_RS06105 0.49 +0.0 1,284,389 + BWI76_RS06105 0.64 +0.4 1,284,402 - BWI76_RS06105 0.65 -0.2 1,284,461 - BWI76_RS06105 0.70 +0.8 1,284,466 - BWI76_RS06105 0.70 +0.5 1,284,510 - BWI76_RS06105 0.74 -1.0 1,284,512 + BWI76_RS06105 0.74 -2.5 1,284,660 - BWI76_RS06105 0.86 -0.7 1,284,722 + -0.0 1,284,780 - -0.4 1,284,793 + +0.5 1,284,840 + +0.1 1,285,266 + BWI76_RS06110 0.38 +0.5 1,285,266 + BWI76_RS06110 0.38 +1.2 1,285,266 + BWI76_RS06110 0.38 +0.5 1,285,266 + BWI76_RS06110 0.38 -1.5 1,285,274 - BWI76_RS06110 0.38 +1.2 1,285,482 + BWI76_RS06110 0.57 +2.0 1,285,944 - -2.6 1,286,548 - BWI76_RS06120 0.19 +1.0 1,286,553 - BWI76_RS06120 0.21 -2.1 1,286,766 + +1.0
Or see this region's nucleotide sequence