Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05745 and BWI76_RS05750 are separated by 72 nucleotides BWI76_RS05750 and BWI76_RS05755 are separated by 270 nucleotides
BWI76_RS05745: BWI76_RS05745 - hypothetical protein, at 1,208,042 to 1,209,280
_RS05745
BWI76_RS05750: BWI76_RS05750 - thiamine biosynthesis protein ThiJ, at 1,209,353 to 1,210,048
_RS05750
BWI76_RS05755: BWI76_RS05755 - beta-ketoacyl-[acyl-carrier-protein] synthase II, at 1,210,319 to 1,211,590
_RS05755
Position (kb)
1209
1210
1211 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1209.263 kb on + strand at 1209.461 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.473 kb on + strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.481 kb on - strand, within BWI76_RS05750 at 1209.610 kb on + strand, within BWI76_RS05750 at 1209.616 kb on - strand, within BWI76_RS05750 at 1209.639 kb on + strand, within BWI76_RS05750 at 1209.703 kb on + strand, within BWI76_RS05750 at 1209.736 kb on + strand, within BWI76_RS05750 at 1209.817 kb on + strand, within BWI76_RS05750 at 1209.820 kb on - strand, within BWI76_RS05750 at 1209.825 kb on - strand, within BWI76_RS05750 at 1209.870 kb on - strand, within BWI76_RS05750 at 1209.921 kb on - strand, within BWI76_RS05750 at 1209.967 kb on - strand, within BWI76_RS05750 at 1209.994 kb on + strand at 1209.994 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.012 kb on + strand at 1210.020 kb on - strand at 1210.105 kb on + strand at 1210.107 kb on + strand at 1210.113 kb on - strand at 1210.116 kb on + strand at 1210.131 kb on + strand at 1210.139 kb on - strand at 1210.175 kb on - strand at 1210.280 kb on - strand at 1210.421 kb on + strand at 1210.428 kb on - strand at 1210.507 kb on - strand, within BWI76_RS05755 at 1210.655 kb on - strand, within BWI76_RS05755 at 1211.043 kb on + strand, within BWI76_RS05755
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 1,209,263 + -1.0 1,209,461 + BWI76_RS05750 0.16 +1.2 1,209,473 + BWI76_RS05750 0.17 -1.7 1,209,473 + BWI76_RS05750 0.17 +0.9 1,209,481 - BWI76_RS05750 0.18 +0.4 1,209,481 - BWI76_RS05750 0.18 -0.9 1,209,481 - BWI76_RS05750 0.18 -0.4 1,209,610 + BWI76_RS05750 0.37 +0.6 1,209,616 - BWI76_RS05750 0.38 +0.5 1,209,639 + BWI76_RS05750 0.41 -0.2 1,209,703 + BWI76_RS05750 0.50 -0.5 1,209,736 + BWI76_RS05750 0.55 -0.4 1,209,817 + BWI76_RS05750 0.67 +0.3 1,209,820 - BWI76_RS05750 0.67 +0.5 1,209,825 - BWI76_RS05750 0.68 +1.6 1,209,870 - BWI76_RS05750 0.74 -1.4 1,209,921 - BWI76_RS05750 0.82 -0.1 1,209,967 - BWI76_RS05750 0.88 -0.9 1,209,994 + -1.5 1,209,994 + -0.3 1,210,012 + -1.6 1,210,012 + -0.7 1,210,012 + +0.2 1,210,020 - +1.7 1,210,105 + +0.9 1,210,107 + -0.7 1,210,113 - -1.0 1,210,116 + +0.2 1,210,131 + -0.9 1,210,139 - +0.4 1,210,175 - -2.1 1,210,280 - -0.2 1,210,421 + +0.5 1,210,428 - -1.1 1,210,507 - BWI76_RS05755 0.15 -0.6 1,210,655 - BWI76_RS05755 0.26 -0.0 1,211,043 + BWI76_RS05755 0.57 +2.5
Or see this region's nucleotide sequence