Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05055 and BWI76_RS05060 overlap by 4 nucleotides BWI76_RS05060 and BWI76_RS05065 overlap by 4 nucleotides BWI76_RS05065 and BWI76_RS05070 overlap by 4 nucleotides BWI76_RS05070 and BWI76_RS05075 overlap by 1 nucleotides BWI76_RS05075 and BWI76_RS05080 are separated by 20 nucleotides
BWI76_RS05055: BWI76_RS05055 - type II secretion system protein K, at 1,059,698 to 1,060,678
_RS05055
BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271
_RS05060
BWI76_RS05065: BWI76_RS05065 - type II secretion system protein GspI, at 1,061,268 to 1,061,633
_RS05065
BWI76_RS05070: BWI76_RS05070 - type II secretion system protein GspH, at 1,061,630 to 1,062,142
_RS05070
BWI76_RS05075: BWI76_RS05075 - type II secretion system protein GspG, at 1,062,142 to 1,062,564
_RS05075
BWI76_RS05080: BWI76_RS05080 - type II secretion protein F, at 1,062,585 to 1,063,790
_RS05080
Position (kb) 1061
1062
1063 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1060.649 kb on - strand at 1060.776 kb on + strand, within BWI76_RS05060 at 1060.835 kb on + strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.854 kb on + strand, within BWI76_RS05060 at 1060.879 kb on + strand, within BWI76_RS05060 at 1060.979 kb on - strand, within BWI76_RS05060 at 1061.022 kb on + strand, within BWI76_RS05060 at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.252 kb on - strand at 1061.451 kb on - strand, within BWI76_RS05065 at 1061.780 kb on - strand, within BWI76_RS05070 at 1062.031 kb on - strand, within BWI76_RS05070 at 1062.129 kb on - strand at 1062.201 kb on + strand, within BWI76_RS05075 at 1062.687 kb on - strand at 1062.687 kb on - strand at 1062.687 kb on - strand at 1062.845 kb on + strand, within BWI76_RS05080 at 1062.942 kb on - strand, within BWI76_RS05080 at 1063.081 kb on + strand, within BWI76_RS05080
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 1,060,649 - -0.2 1,060,776 + BWI76_RS05060 0.17 -2.0 1,060,835 + BWI76_RS05060 0.27 +2.2 1,060,850 - BWI76_RS05060 0.29 +1.0 1,060,850 - BWI76_RS05060 0.29 +0.1 1,060,850 - BWI76_RS05060 0.29 +0.9 1,060,854 + BWI76_RS05060 0.30 +1.2 1,060,879 + BWI76_RS05060 0.34 -2.6 1,060,979 - BWI76_RS05060 0.51 +1.9 1,061,022 + BWI76_RS05060 0.58 +0.8 1,061,244 + +0.4 1,061,244 + -0.0 1,061,244 + +0.9 1,061,252 - -2.6 1,061,451 - BWI76_RS05065 0.50 -1.5 1,061,780 - BWI76_RS05070 0.29 -1.2 1,062,031 - BWI76_RS05070 0.78 -1.0 1,062,129 - -0.5 1,062,201 + BWI76_RS05075 0.14 +0.7 1,062,687 - +0.8 1,062,687 - +1.2 1,062,687 - -0.3 1,062,845 + BWI76_RS05080 0.22 +0.3 1,062,942 - BWI76_RS05080 0.30 +0.9 1,063,081 + BWI76_RS05080 0.41 +1.4
Or see this region's nucleotide sequence