Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03015 and BWI76_RS03020 are separated by 52 nucleotides BWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotides BWI76_RS03025 and BWI76_RS03030 are separated by 123 nucleotides
BWI76_RS03015: BWI76_RS03015 - RNase E inhibitor protein, at 617,067 to 617,492
_RS03015
BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303
_RS03020
BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906
_RS03025
BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885
_RS03030
Position (kb) 618
619 Strain fitness (log2 ratio) -2
-1
0
1
2 at 617.607 kb on - strand at 617.654 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.665 kb on - strand, within BWI76_RS03020 at 617.761 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 617.995 kb on - strand, within BWI76_RS03020 at 618.331 kb on - strand at 618.410 kb on + strand at 618.565 kb on + strand, within BWI76_RS03025 at 618.573 kb on - strand, within BWI76_RS03025 at 618.605 kb on - strand, within BWI76_RS03025 at 618.884 kb on - strand at 618.885 kb on - strand at 618.966 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 617,607 - -0.1 617,654 - BWI76_RS03020 0.14 -0.4 617,665 - BWI76_RS03020 0.16 +2.0 617,665 - BWI76_RS03020 0.16 +0.6 617,761 - BWI76_RS03020 0.28 -1.1 617,995 - BWI76_RS03020 0.59 -0.8 617,995 - BWI76_RS03020 0.59 -1.2 618,331 - -1.2 618,410 + -1.4 618,565 + BWI76_RS03025 0.32 -0.2 618,573 - BWI76_RS03025 0.34 -2.5 618,605 - BWI76_RS03025 0.40 +1.8 618,884 - +0.1 618,885 - -1.0 618,966 + +1.2
Or see this region's nucleotide sequence