Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01260 and BWI76_RS01265 are separated by 43 nucleotides BWI76_RS01265 and BWI76_RS01270 are separated by 88 nucleotides BWI76_RS01270 and BWI76_RS01275 overlap by 113 nucleotides
BWI76_RS01260: BWI76_RS01260 - lysophospholipase L2, at 258,083 to 259,075
_RS01260
BWI76_RS01265: BWI76_RS01265 - sugar/pyridoxal phosphate phosphatase YigL, at 259,119 to 259,919
_RS01265
BWI76_RS01270: BWI76_RS01270 - hypothetical protein, at 260,008 to 260,907
_RS01270
BWI76_RS01275: BWI76_RS01275 - LysR family transcriptional regulator, at 260,795 to 261,748
_RS01275
Position (kb) 259
260 Strain fitness (log2 ratio) -2
-1
0
1
2 at 258.332 kb on - strand, within BWI76_RS01260 at 258.485 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.628 kb on - strand, within BWI76_RS01260 at 258.689 kb on + strand, within BWI76_RS01260 at 258.907 kb on - strand, within BWI76_RS01260 at 258.955 kb on - strand, within BWI76_RS01260 at 258.963 kb on + strand, within BWI76_RS01260 at 259.069 kb on + strand at 259.099 kb on + strand at 259.099 kb on + strand at 259.186 kb on + strand at 259.508 kb on - strand, within BWI76_RS01265 at 259.635 kb on + strand, within BWI76_RS01265 at 259.660 kb on + strand, within BWI76_RS01265 at 259.701 kb on - strand, within BWI76_RS01265 at 259.750 kb on - strand, within BWI76_RS01265 at 259.760 kb on + strand, within BWI76_RS01265 at 259.769 kb on - strand, within BWI76_RS01265 at 259.806 kb on + strand, within BWI76_RS01265 at 259.846 kb on - strand at 260.025 kb on - strand at 260.056 kb on - strand at 260.125 kb on + strand, within BWI76_RS01270 at 260.163 kb on + strand, within BWI76_RS01270 at 260.184 kb on + strand, within BWI76_RS01270 at 260.206 kb on - strand, within BWI76_RS01270 at 260.450 kb on + strand, within BWI76_RS01270 at 260.451 kb on + strand, within BWI76_RS01270 at 260.459 kb on - strand, within BWI76_RS01270 at 260.522 kb on - strand, within BWI76_RS01270 at 260.522 kb on - strand, within BWI76_RS01270 at 260.578 kb on - strand, within BWI76_RS01270
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 258,332 - BWI76_RS01260 0.25 -1.3 258,485 + BWI76_RS01260 0.40 +0.6 258,620 + BWI76_RS01260 0.54 +0.0 258,620 + BWI76_RS01260 0.54 +0.6 258,628 - BWI76_RS01260 0.55 +0.6 258,689 + BWI76_RS01260 0.61 +0.2 258,907 - BWI76_RS01260 0.83 +1.4 258,955 - BWI76_RS01260 0.88 +1.7 258,963 + BWI76_RS01260 0.89 +1.3 259,069 + -0.1 259,099 + -1.4 259,099 + +0.1 259,186 + +0.1 259,508 - BWI76_RS01265 0.49 +0.6 259,635 + BWI76_RS01265 0.64 +1.7 259,660 + BWI76_RS01265 0.68 +0.2 259,701 - BWI76_RS01265 0.73 -0.3 259,750 - BWI76_RS01265 0.79 +0.3 259,760 + BWI76_RS01265 0.80 +2.1 259,769 - BWI76_RS01265 0.81 -2.0 259,806 + BWI76_RS01265 0.86 +0.6 259,846 - -0.1 260,025 - +1.6 260,056 - +0.9 260,125 + BWI76_RS01270 0.13 -0.3 260,163 + BWI76_RS01270 0.17 -0.1 260,184 + BWI76_RS01270 0.20 -1.1 260,206 - BWI76_RS01270 0.22 -0.3 260,450 + BWI76_RS01270 0.49 -0.0 260,451 + BWI76_RS01270 0.49 -1.6 260,459 - BWI76_RS01270 0.50 -0.5 260,522 - BWI76_RS01270 0.57 +0.9 260,522 - BWI76_RS01270 0.57 +0.1 260,578 - BWI76_RS01270 0.63 -0.8
Or see this region's nucleotide sequence