Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01040

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01035 and BWI76_RS01040 are separated by 221 nucleotidesBWI76_RS01040 and BWI76_RS01045 are separated by 35 nucleotidesBWI76_RS01045 and BWI76_RS01050 are separated by 61 nucleotides BWI76_RS01035: BWI76_RS01035 - ADP-ribosylation/crystallin J1, at 211,895 to 212,911 _RS01035 BWI76_RS01040: BWI76_RS01040 - transcriptional regulator, at 213,133 to 213,918 _RS01040 BWI76_RS01045: BWI76_RS01045 - transporter, at 213,954 to 214,676 _RS01045 BWI76_RS01050: BWI76_RS01050 - o-pyrocatechuate decarboxylase, at 214,738 to 215,736 _RS01050 Position (kb) 213 214Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 212.493 kb on + strand, within BWI76_RS01035at 212.568 kb on - strand, within BWI76_RS01035at 212.690 kb on + strand, within BWI76_RS01035at 212.699 kb on + strand, within BWI76_RS01035at 212.703 kb on - strand, within BWI76_RS01035at 212.707 kb on - strand, within BWI76_RS01035at 212.815 kb on + strandat 212.815 kb on + strandat 212.823 kb on + strandat 213.173 kb on + strandat 213.192 kb on + strandat 213.192 kb on + strandat 213.192 kb on + strandat 213.204 kb on + strandat 213.290 kb on + strand, within BWI76_RS01040at 213.298 kb on - strand, within BWI76_RS01040at 213.315 kb on + strand, within BWI76_RS01040at 213.315 kb on + strand, within BWI76_RS01040at 213.336 kb on + strand, within BWI76_RS01040at 213.398 kb on + strand, within BWI76_RS01040at 213.398 kb on + strand, within BWI76_RS01040at 213.398 kb on + strand, within BWI76_RS01040at 213.406 kb on - strand, within BWI76_RS01040at 213.406 kb on - strandat 213.406 kb on - strand, within BWI76_RS01040at 213.527 kb on + strand, within BWI76_RS01040at 213.556 kb on + strand, within BWI76_RS01040at 213.556 kb on + strand, within BWI76_RS01040at 213.561 kb on + strand, within BWI76_RS01040at 213.562 kb on + strand, within BWI76_RS01040at 213.696 kb on + strand, within BWI76_RS01040at 213.770 kb on - strand, within BWI76_RS01040at 213.782 kb on + strand, within BWI76_RS01040at 213.803 kb on - strand, within BWI76_RS01040at 213.815 kb on + strand, within BWI76_RS01040at 213.821 kb on + strand, within BWI76_RS01040at 213.821 kb on + strand, within BWI76_RS01040at 213.829 kb on - strandat 213.864 kb on + strandat 213.870 kb on + strandat 213.878 kb on - strandat 213.887 kb on + strandat 213.948 kb on - strandat 214.011 kb on - strandat 214.057 kb on - strandat 214.057 kb on - strand, within BWI76_RS01045at 214.058 kb on - strand, within BWI76_RS01045at 214.095 kb on + strand, within BWI76_RS01045at 214.104 kb on + strand, within BWI76_RS01045at 214.116 kb on + strand, within BWI76_RS01045at 214.117 kb on + strand, within BWI76_RS01045at 214.122 kb on - strand, within BWI76_RS01045at 214.122 kb on - strand, within BWI76_RS01045at 214.127 kb on + strand, within BWI76_RS01045at 214.144 kb on + strand, within BWI76_RS01045at 214.194 kb on + strand, within BWI76_RS01045at 214.200 kb on + strand, within BWI76_RS01045at 214.202 kb on - strand, within BWI76_RS01045at 214.202 kb on - strand, within BWI76_RS01045at 214.203 kb on - strand, within BWI76_RS01045at 214.203 kb on - strand, within BWI76_RS01045at 214.208 kb on - strand, within BWI76_RS01045at 214.208 kb on - strand, within BWI76_RS01045at 214.212 kb on + strand, within BWI76_RS01045at 214.220 kb on - strand, within BWI76_RS01045at 214.259 kb on + strand, within BWI76_RS01045at 214.278 kb on - strand, within BWI76_RS01045at 214.336 kb on - strand, within BWI76_RS01045at 214.339 kb on + strand, within BWI76_RS01045at 214.340 kb on - strand, within BWI76_RS01045at 214.347 kb on - strand, within BWI76_RS01045at 214.347 kb on - strand, within BWI76_RS01045at 214.347 kb on - strand, within BWI76_RS01045at 214.347 kb on - strand, within BWI76_RS01045at 214.347 kb on - strand, within BWI76_RS01045at 214.347 kb on - strand, within BWI76_RS01045at 214.354 kb on + strand, within BWI76_RS01045at 214.362 kb on - strandat 214.362 kb on - strand, within BWI76_RS01045at 214.367 kb on + strand, within BWI76_RS01045at 214.372 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.378 kb on + strand, within BWI76_RS01045at 214.386 kb on - strandat 214.386 kb on - strand, within BWI76_RS01045at 214.386 kb on - strand, within BWI76_RS01045at 214.386 kb on - strand, within BWI76_RS01045at 214.389 kb on + strand, within BWI76_RS01045at 214.400 kb on - strand, within BWI76_RS01045at 214.414 kb on + strand, within BWI76_RS01045at 214.419 kb on - strand, within BWI76_RS01045at 214.453 kb on - strand, within BWI76_RS01045at 214.454 kb on - strand, within BWI76_RS01045at 214.458 kb on + strand, within BWI76_RS01045at 214.482 kb on + strand, within BWI76_RS01045at 214.490 kb on - strand, within BWI76_RS01045at 214.556 kb on - strand, within BWI76_RS01045at 214.625 kb on + strandat 214.635 kb on + strandat 214.635 kb on + strandat 214.635 kb on + strandat 214.635 kb on + strandat 214.640 kb on + strandat 214.641 kb on + strandat 214.643 kb on + strandat 214.643 kb on - strandat 214.643 kb on - strandat 214.643 kb on - strandat 214.643 kb on - strandat 214.643 kb on - strandat 214.643 kb on - strandat 214.643 kb on - strandat 214.648 kb on - strandat 214.649 kb on - strandat 214.651 kb on - strandat 214.685 kb on - strandat 214.763 kb on + strandat 214.763 kb on + strandat 214.768 kb on + strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.771 kb on - strandat 214.828 kb on + strandat 214.836 kb on - strandat 214.836 kb on - strandat 214.842 kb on + strand, within BWI76_RS01050at 214.851 kb on - strand, within BWI76_RS01050at 214.857 kb on - strand, within BWI76_RS01050at 214.857 kb on - strand, within BWI76_RS01050at 214.867 kb on - strand, within BWI76_RS01050at 214.874 kb on + strand, within BWI76_RS01050at 214.880 kb on + strand, within BWI76_RS01050at 214.884 kb on - strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050at 214.916 kb on + strand, within BWI76_RS01050

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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212,493 + BWI76_RS01035 0.59 +1.5
212,568 - BWI76_RS01035 0.66 -1.5
212,690 + BWI76_RS01035 0.78 +1.9
212,699 + BWI76_RS01035 0.79 +0.8
212,703 - BWI76_RS01035 0.79 +0.3
212,707 - BWI76_RS01035 0.80 -0.1
212,815 + -0.1
212,815 + -1.3
212,823 + +0.2
213,173 + +0.0
213,192 + +1.7
213,192 + +1.5
213,192 + -0.1
213,204 + -4.1
213,290 + BWI76_RS01040 0.20 -0.4
213,298 - BWI76_RS01040 0.21 +0.1
213,315 + BWI76_RS01040 0.23 -0.1
213,315 + BWI76_RS01040 0.23 +2.5
213,336 + BWI76_RS01040 0.26 -1.3
213,398 + BWI76_RS01040 0.34 +1.3
213,398 + BWI76_RS01040 0.34 +1.1
213,398 + BWI76_RS01040 0.34 +2.0
213,406 - BWI76_RS01040 0.35 -0.1
213,406 - -0.8
213,406 - BWI76_RS01040 0.35 -1.4
213,527 + BWI76_RS01040 0.50 -0.6
213,556 + BWI76_RS01040 0.54 -0.5
213,556 + BWI76_RS01040 0.54 +1.9
213,561 + BWI76_RS01040 0.54 +0.6
213,562 + BWI76_RS01040 0.55 +0.7
213,696 + BWI76_RS01040 0.72 -1.5
213,770 - BWI76_RS01040 0.81 +1.1
213,782 + BWI76_RS01040 0.83 -0.2
213,803 - BWI76_RS01040 0.85 -1.1
213,815 + BWI76_RS01040 0.87 +0.5
213,821 + BWI76_RS01040 0.88 -0.8
213,821 + BWI76_RS01040 0.88 -1.3
213,829 - -0.5
213,864 + -1.9
213,870 + +1.2
213,878 - +0.1
213,887 + -0.1
213,948 - +0.5
214,011 - +0.2
214,057 - +0.5
214,057 - BWI76_RS01045 0.14 +0.2
214,058 - BWI76_RS01045 0.14 +0.2
214,095 + BWI76_RS01045 0.20 -0.4
214,104 + BWI76_RS01045 0.21 -0.4
214,116 + BWI76_RS01045 0.22 +2.3
214,117 + BWI76_RS01045 0.23 +0.3
214,122 - BWI76_RS01045 0.23 -0.9
214,122 - BWI76_RS01045 0.23 -1.2
214,127 + BWI76_RS01045 0.24 +0.1
214,144 + BWI76_RS01045 0.26 +0.7
214,194 + BWI76_RS01045 0.33 -0.3
214,200 + BWI76_RS01045 0.34 -0.1
214,202 - BWI76_RS01045 0.34 +0.2
214,202 - BWI76_RS01045 0.34 +0.3
214,203 - BWI76_RS01045 0.34 -0.2
214,203 - BWI76_RS01045 0.34 +0.2
214,208 - BWI76_RS01045 0.35 +0.8
214,208 - BWI76_RS01045 0.35 +0.5
214,212 + BWI76_RS01045 0.36 -0.0
214,220 - BWI76_RS01045 0.37 +0.4
214,259 + BWI76_RS01045 0.42 -0.5
214,278 - BWI76_RS01045 0.45 +0.0
214,336 - BWI76_RS01045 0.53 -2.9
214,339 + BWI76_RS01045 0.53 -0.6
214,340 - BWI76_RS01045 0.53 -1.6
214,347 - BWI76_RS01045 0.54 +1.5
214,347 - BWI76_RS01045 0.54 +2.7
214,347 - BWI76_RS01045 0.54 -1.2
214,347 - BWI76_RS01045 0.54 -1.0
214,347 - BWI76_RS01045 0.54 -0.2
214,347 - BWI76_RS01045 0.54 +1.3
214,354 + BWI76_RS01045 0.55 +0.5
214,362 - +1.8
214,362 - BWI76_RS01045 0.56 -0.0
214,367 + BWI76_RS01045 0.57 +2.5
214,372 + BWI76_RS01045 0.58 +1.5
214,378 + BWI76_RS01045 0.59 +0.0
214,378 + BWI76_RS01045 0.59 -0.8
214,378 + BWI76_RS01045 0.59 -2.2
214,378 + BWI76_RS01045 0.59 +0.8
214,378 + BWI76_RS01045 0.59 -0.1
214,378 + BWI76_RS01045 0.59 +2.5
214,378 + BWI76_RS01045 0.59 +0.8
214,386 - -1.3
214,386 - BWI76_RS01045 0.60 +0.0
214,386 - BWI76_RS01045 0.60 -0.8
214,386 - BWI76_RS01045 0.60 +0.9
214,389 + BWI76_RS01045 0.60 -0.0
214,400 - BWI76_RS01045 0.62 +1.1
214,414 + BWI76_RS01045 0.64 -3.1
214,419 - BWI76_RS01045 0.64 -0.5
214,453 - BWI76_RS01045 0.69 -1.5
214,454 - BWI76_RS01045 0.69 -0.3
214,458 + BWI76_RS01045 0.70 +0.8
214,482 + BWI76_RS01045 0.73 +1.0
214,490 - BWI76_RS01045 0.74 +0.8
214,556 - BWI76_RS01045 0.83 -0.6
214,625 + -0.5
214,635 + +1.5
214,635 + +1.8
214,635 + +0.8
214,635 + -0.3
214,640 + +1.4
214,641 + -2.6
214,643 + +0.2
214,643 - +1.7
214,643 - +0.3
214,643 - -1.6
214,643 - -0.7
214,643 - +2.3
214,643 - -2.4
214,643 - -2.2
214,648 - -2.4
214,649 - +1.0
214,651 - +1.5
214,685 - -1.5
214,763 + +1.5
214,763 + +0.7
214,768 + +0.9
214,771 - -0.6
214,771 - -1.3
214,771 - +0.6
214,771 - +0.3
214,771 - +0.4
214,771 - +0.8
214,771 - -1.9
214,771 - +0.0
214,771 - +1.7
214,771 - +1.3
214,771 - +0.7
214,828 + +0.9
214,836 - +1.5
214,836 - +0.3
214,842 + BWI76_RS01050 0.10 +1.5
214,851 - BWI76_RS01050 0.11 -3.4
214,857 - BWI76_RS01050 0.12 +1.2
214,857 - BWI76_RS01050 0.12 +0.6
214,867 - BWI76_RS01050 0.13 +0.7
214,874 + BWI76_RS01050 0.14 +1.5
214,880 + BWI76_RS01050 0.14 +2.3
214,884 - BWI76_RS01050 0.15 -0.9
214,916 + BWI76_RS01050 0.18 +1.5
214,916 + BWI76_RS01050 0.18 -0.6
214,916 + BWI76_RS01050 0.18 +1.5
214,916 + BWI76_RS01050 0.18 +0.4
214,916 + BWI76_RS01050 0.18 +1.5
214,916 + BWI76_RS01050 0.18 -1.5
214,916 + BWI76_RS01050 0.18 -0.9
214,916 + BWI76_RS01050 0.18 -1.3

Or see this region's nucleotide sequence