Experiment: KLEB20 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotides BWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotides BWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides
BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709
_RS00075
BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581
_RS00080
BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042
_RS00085
BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393
_RS00090
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 16.009 kb on + strand, within BWI76_RS00080 at 16.225 kb on + strand, within BWI76_RS00080 at 16.451 kb on + strand, within BWI76_RS00080 at 16.461 kb on + strand, within BWI76_RS00080 at 16.469 kb on - strand, within BWI76_RS00080 at 16.514 kb on - strand at 16.529 kb on - strand at 16.601 kb on - strand at 16.609 kb on + strand at 16.616 kb on + strand at 16.631 kb on + strand, within BWI76_RS00085 at 16.631 kb on + strand, within BWI76_RS00085 at 16.932 kb on - strand, within BWI76_RS00085 at 17.020 kb on - strand at 17.088 kb on - strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.184 kb on - strand at 17.184 kb on - strand at 17.214 kb on - strand at 17.244 kb on + strand at 17.252 kb on - strand at 17.252 kb on - strand at 17.498 kb on - strand, within BWI76_RS00090 at 17.539 kb on + strand, within BWI76_RS00090 at 17.546 kb on + strand, within BWI76_RS00090 at 17.672 kb on + strand, within BWI76_RS00090 at 17.683 kb on + strand, within BWI76_RS00090 at 17.688 kb on + strand, within BWI76_RS00090 at 17.696 kb on - strand, within BWI76_RS00090 at 17.881 kb on - strand, within BWI76_RS00090 at 17.963 kb on + strand, within BWI76_RS00090 at 18.025 kb on - strand, within BWI76_RS00090
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB20 MOI 1 remove 16,009 + BWI76_RS00080 0.35 -0.3 16,225 + BWI76_RS00080 0.59 -1.8 16,451 + BWI76_RS00080 0.85 +0.1 16,461 + BWI76_RS00080 0.86 -0.4 16,469 - BWI76_RS00080 0.87 -0.5 16,514 - -1.1 16,529 - +2.1 16,601 - +0.2 16,609 + -0.9 16,616 + +1.1 16,631 + BWI76_RS00085 0.11 -0.4 16,631 + BWI76_RS00085 0.11 -1.5 16,932 - BWI76_RS00085 0.76 -2.1 17,020 - -0.3 17,088 - -0.5 17,176 + +0.5 17,176 + +0.1 17,176 + +0.7 17,176 + -0.5 17,184 - +1.0 17,184 - +0.1 17,214 - +0.2 17,244 + -1.2 17,252 - +0.3 17,252 - -1.1 17,498 - BWI76_RS00090 0.24 -3.0 17,539 + BWI76_RS00090 0.28 +0.1 17,546 + BWI76_RS00090 0.28 -3.7 17,672 + BWI76_RS00090 0.39 -1.0 17,683 + BWI76_RS00090 0.40 -2.6 17,688 + BWI76_RS00090 0.40 +0.1 17,696 - BWI76_RS00090 0.41 -0.7 17,881 - BWI76_RS00090 0.57 +0.4 17,963 + BWI76_RS00090 0.64 -0.4 18,025 - BWI76_RS00090 0.69 -0.2
Or see this region's nucleotide sequence