Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00085

Experiment: KLEB20 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotidesBWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotidesBWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709 _RS00075 BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581 _RS00080 BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042 _RS00085 BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393 _RS00090 Position (kb) 16 17 18Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 16.009 kb on + strand, within BWI76_RS00080at 16.225 kb on + strand, within BWI76_RS00080at 16.451 kb on + strand, within BWI76_RS00080at 16.461 kb on + strand, within BWI76_RS00080at 16.469 kb on - strand, within BWI76_RS00080at 16.514 kb on - strandat 16.529 kb on - strandat 16.601 kb on - strandat 16.609 kb on + strandat 16.616 kb on + strandat 16.631 kb on + strand, within BWI76_RS00085at 16.631 kb on + strand, within BWI76_RS00085at 16.932 kb on - strand, within BWI76_RS00085at 17.020 kb on - strandat 17.088 kb on - strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.176 kb on + strandat 17.184 kb on - strandat 17.184 kb on - strandat 17.214 kb on - strandat 17.244 kb on + strandat 17.252 kb on - strandat 17.252 kb on - strandat 17.498 kb on - strand, within BWI76_RS00090at 17.539 kb on + strand, within BWI76_RS00090at 17.546 kb on + strand, within BWI76_RS00090at 17.672 kb on + strand, within BWI76_RS00090at 17.683 kb on + strand, within BWI76_RS00090at 17.688 kb on + strand, within BWI76_RS00090at 17.696 kb on - strand, within BWI76_RS00090at 17.881 kb on - strand, within BWI76_RS00090at 17.963 kb on + strand, within BWI76_RS00090at 18.025 kb on - strand, within BWI76_RS00090

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB20 MOI 1
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16,009 + BWI76_RS00080 0.35 -0.3
16,225 + BWI76_RS00080 0.59 -1.8
16,451 + BWI76_RS00080 0.85 +0.1
16,461 + BWI76_RS00080 0.86 -0.4
16,469 - BWI76_RS00080 0.87 -0.5
16,514 - -1.1
16,529 - +2.1
16,601 - +0.2
16,609 + -0.9
16,616 + +1.1
16,631 + BWI76_RS00085 0.11 -0.4
16,631 + BWI76_RS00085 0.11 -1.5
16,932 - BWI76_RS00085 0.76 -2.1
17,020 - -0.3
17,088 - -0.5
17,176 + +0.5
17,176 + +0.1
17,176 + +0.7
17,176 + -0.5
17,184 - +1.0
17,184 - +0.1
17,214 - +0.2
17,244 + -1.2
17,252 - +0.3
17,252 - -1.1
17,498 - BWI76_RS00090 0.24 -3.0
17,539 + BWI76_RS00090 0.28 +0.1
17,546 + BWI76_RS00090 0.28 -3.7
17,672 + BWI76_RS00090 0.39 -1.0
17,683 + BWI76_RS00090 0.40 -2.6
17,688 + BWI76_RS00090 0.40 +0.1
17,696 - BWI76_RS00090 0.41 -0.7
17,881 - BWI76_RS00090 0.57 +0.4
17,963 + BWI76_RS00090 0.64 -0.4
18,025 - BWI76_RS00090 0.69 -0.2

Or see this region's nucleotide sequence