Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12575

Experiment: KLEB17 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12565 and BWI76_RS12570 overlap by 4 nucleotidesBWI76_RS12570 and BWI76_RS12575 are separated by 1 nucleotidesBWI76_RS12575 and BWI76_RS12580 overlap by 4 nucleotidesBWI76_RS12580 and BWI76_RS12585 overlap by 4 nucleotides BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734 _RS12565 BWI76_RS12570: BWI76_RS12570 - DUF1365 domain-containing protein, at 2,624,731 to 2,625,453 _RS12570 BWI76_RS12575: BWI76_RS12575 - FAD-dependent oxidoreductase, at 2,625,455 to 2,626,714 _RS12575 BWI76_RS12580: BWI76_RS12580 - oxidoreductase, at 2,626,711 to 2,627,430 _RS12580 BWI76_RS12585: BWI76_RS12585 - transcriptional regulator, at 2,627,427 to 2,627,861 _RS12585 Position (kb) 2625 2626 2627Strain fitness (log2 ratio) -2 -1 0 1at 2624.495 kb on - strand, within BWI76_RS12565at 2624.791 kb on - strandat 2624.865 kb on + strand, within BWI76_RS12570at 2625.129 kb on + strand, within BWI76_RS12570at 2625.533 kb on - strandat 2625.567 kb on - strandat 2625.891 kb on + strand, within BWI76_RS12575at 2625.925 kb on - strand, within BWI76_RS12575at 2626.238 kb on - strand, within BWI76_RS12575at 2626.278 kb on - strand, within BWI76_RS12575at 2626.640 kb on + strandat 2626.709 kb on + strandat 2626.785 kb on + strand, within BWI76_RS12580at 2626.786 kb on + strand, within BWI76_RS12580at 2626.998 kb on - strand, within BWI76_RS12580at 2627.336 kb on - strand, within BWI76_RS12580at 2627.404 kb on + strandat 2627.610 kb on + strand, within BWI76_RS12585

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 0.1
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2,624,495 - BWI76_RS12565 0.80 +0.5
2,624,791 - +1.2
2,624,865 + BWI76_RS12570 0.19 -0.4
2,625,129 + BWI76_RS12570 0.55 -2.0
2,625,533 - +0.3
2,625,567 - +0.3
2,625,891 + BWI76_RS12575 0.35 -2.6
2,625,925 - BWI76_RS12575 0.37 -2.2
2,626,238 - BWI76_RS12575 0.62 -1.0
2,626,278 - BWI76_RS12575 0.65 -0.6
2,626,640 + +0.3
2,626,709 + -0.6
2,626,785 + BWI76_RS12580 0.10 +0.1
2,626,786 + BWI76_RS12580 0.10 +0.6
2,626,998 - BWI76_RS12580 0.40 +0.2
2,627,336 - BWI76_RS12580 0.87 -0.3
2,627,404 + -0.9
2,627,610 + BWI76_RS12585 0.42 -0.2

Or see this region's nucleotide sequence