Experiment: KLEB17 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12565 and BWI76_RS12570 overlap by 4 nucleotides BWI76_RS12570 and BWI76_RS12575 are separated by 1 nucleotides BWI76_RS12575 and BWI76_RS12580 overlap by 4 nucleotides BWI76_RS12580 and BWI76_RS12585 overlap by 4 nucleotides
BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734
_RS12565
BWI76_RS12570: BWI76_RS12570 - DUF1365 domain-containing protein, at 2,624,731 to 2,625,453
_RS12570
BWI76_RS12575: BWI76_RS12575 - FAD-dependent oxidoreductase, at 2,625,455 to 2,626,714
_RS12575
BWI76_RS12580: BWI76_RS12580 - oxidoreductase, at 2,626,711 to 2,627,430
_RS12580
BWI76_RS12585: BWI76_RS12585 - transcriptional regulator, at 2,627,427 to 2,627,861
_RS12585
Position (kb)
2625
2626
2627 Strain fitness (log2 ratio)
-2
-1
0
1 at 2624.495 kb on - strand, within BWI76_RS12565 at 2624.791 kb on - strand at 2624.865 kb on + strand, within BWI76_RS12570 at 2625.129 kb on + strand, within BWI76_RS12570 at 2625.533 kb on - strand at 2625.567 kb on - strand at 2625.891 kb on + strand, within BWI76_RS12575 at 2625.925 kb on - strand, within BWI76_RS12575 at 2626.238 kb on - strand, within BWI76_RS12575 at 2626.278 kb on - strand, within BWI76_RS12575 at 2626.640 kb on + strand at 2626.709 kb on + strand at 2626.785 kb on + strand, within BWI76_RS12580 at 2626.786 kb on + strand, within BWI76_RS12580 at 2626.998 kb on - strand, within BWI76_RS12580 at 2627.336 kb on - strand, within BWI76_RS12580 at 2627.404 kb on + strand at 2627.610 kb on + strand, within BWI76_RS12585
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 0.1 remove 2,624,495 - BWI76_RS12565 0.80 +0.5 2,624,791 - +1.2 2,624,865 + BWI76_RS12570 0.19 -0.4 2,625,129 + BWI76_RS12570 0.55 -2.0 2,625,533 - +0.3 2,625,567 - +0.3 2,625,891 + BWI76_RS12575 0.35 -2.6 2,625,925 - BWI76_RS12575 0.37 -2.2 2,626,238 - BWI76_RS12575 0.62 -1.0 2,626,278 - BWI76_RS12575 0.65 -0.6 2,626,640 + +0.3 2,626,709 + -0.6 2,626,785 + BWI76_RS12580 0.10 +0.1 2,626,786 + BWI76_RS12580 0.10 +0.6 2,626,998 - BWI76_RS12580 0.40 +0.2 2,627,336 - BWI76_RS12580 0.87 -0.3 2,627,404 + -0.9 2,627,610 + BWI76_RS12585 0.42 -0.2
Or see this region's nucleotide sequence