Experiment: KLEB17 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27235 and BWI76_RS27240 are separated by 3 nucleotides BWI76_RS27240 and BWI76_RS27245 overlap by 4 nucleotides BWI76_RS27245 and BWI76_RS27250 are separated by 160 nucleotides
BWI76_RS27235: BWI76_RS27235 - murein hydrolase activator EnvC, at 5,652,316 to 5,653,587
_RS27235
BWI76_RS27240: BWI76_RS27240 - hypothetical protein, at 5,653,591 to 5,654,523
_RS27240
BWI76_RS27245: BWI76_RS27245 - glycosyl transferase family protein, at 5,654,520 to 5,655,317
_RS27245
BWI76_RS27250: BWI76_RS27250 - L-threonine 3-dehydrogenase, at 5,655,478 to 5,656,503
_RS27250
Position (kb)
5653
5654
5655 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5652.782 kb on - strand, within BWI76_RS27235 at 5652.842 kb on + strand, within BWI76_RS27235 at 5652.929 kb on - strand, within BWI76_RS27235 at 5653.100 kb on + strand, within BWI76_RS27235 at 5653.122 kb on + strand, within BWI76_RS27235 at 5653.148 kb on + strand, within BWI76_RS27235 at 5653.204 kb on - strand, within BWI76_RS27235 at 5653.266 kb on + strand, within BWI76_RS27235 at 5653.272 kb on + strand, within BWI76_RS27235 at 5653.323 kb on + strand, within BWI76_RS27235 at 5653.454 kb on - strand, within BWI76_RS27235 at 5653.464 kb on + strand at 5653.507 kb on + strand at 5653.562 kb on + strand at 5653.570 kb on - strand at 5653.643 kb on - strand at 5653.763 kb on - strand, within BWI76_RS27240 at 5653.879 kb on + strand, within BWI76_RS27240 at 5653.900 kb on - strand, within BWI76_RS27240 at 5653.903 kb on - strand, within BWI76_RS27240 at 5653.948 kb on - strand, within BWI76_RS27240 at 5653.962 kb on + strand, within BWI76_RS27240 at 5653.965 kb on - strand, within BWI76_RS27240 at 5653.998 kb on - strand, within BWI76_RS27240 at 5654.067 kb on + strand, within BWI76_RS27240 at 5654.085 kb on + strand, within BWI76_RS27240 at 5654.088 kb on - strand, within BWI76_RS27240 at 5654.187 kb on + strand, within BWI76_RS27240 at 5654.195 kb on - strand, within BWI76_RS27240 at 5654.223 kb on + strand, within BWI76_RS27240 at 5654.254 kb on + strand, within BWI76_RS27240 at 5654.254 kb on + strand, within BWI76_RS27240 at 5654.278 kb on + strand, within BWI76_RS27240 at 5654.295 kb on - strand, within BWI76_RS27240 at 5654.332 kb on - strand, within BWI76_RS27240 at 5654.568 kb on + strand at 5654.571 kb on - strand at 5654.588 kb on - strand at 5654.621 kb on + strand, within BWI76_RS27245 at 5654.644 kb on + strand, within BWI76_RS27245 at 5654.673 kb on - strand, within BWI76_RS27245 at 5654.799 kb on + strand, within BWI76_RS27245 at 5654.805 kb on - strand, within BWI76_RS27245 at 5654.807 kb on - strand, within BWI76_RS27245 at 5655.008 kb on - strand, within BWI76_RS27245 at 5655.024 kb on - strand, within BWI76_RS27245 at 5655.038 kb on - strand, within BWI76_RS27245 at 5655.065 kb on + strand, within BWI76_RS27245 at 5655.075 kb on + strand, within BWI76_RS27245 at 5655.094 kb on - strand, within BWI76_RS27245 at 5655.094 kb on - strand, within BWI76_RS27245 at 5655.142 kb on + strand, within BWI76_RS27245 at 5655.258 kb on - strand at 5655.329 kb on + strand at 5655.330 kb on + strand at 5655.339 kb on + strand at 5655.347 kb on - strand at 5655.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 0.1 remove 5,652,782 - BWI76_RS27235 0.37 -0.2 5,652,842 + BWI76_RS27235 0.41 -3.0 5,652,929 - BWI76_RS27235 0.48 -0.9 5,653,100 + BWI76_RS27235 0.62 -0.4 5,653,122 + BWI76_RS27235 0.63 +0.8 5,653,148 + BWI76_RS27235 0.65 -1.2 5,653,204 - BWI76_RS27235 0.70 -1.1 5,653,266 + BWI76_RS27235 0.75 -0.6 5,653,272 + BWI76_RS27235 0.75 +0.3 5,653,323 + BWI76_RS27235 0.79 -1.1 5,653,454 - BWI76_RS27235 0.89 +0.3 5,653,464 + -1.1 5,653,507 + -0.5 5,653,562 + -0.0 5,653,570 - -0.6 5,653,643 - -0.8 5,653,763 - BWI76_RS27240 0.18 -0.1 5,653,879 + BWI76_RS27240 0.31 -0.2 5,653,900 - BWI76_RS27240 0.33 -0.8 5,653,903 - BWI76_RS27240 0.33 +0.6 5,653,948 - BWI76_RS27240 0.38 -2.1 5,653,962 + BWI76_RS27240 0.40 -0.9 5,653,965 - BWI76_RS27240 0.40 -3.3 5,653,998 - BWI76_RS27240 0.44 -1.5 5,654,067 + BWI76_RS27240 0.51 -1.3 5,654,085 + BWI76_RS27240 0.53 +0.1 5,654,088 - BWI76_RS27240 0.53 -0.2 5,654,187 + BWI76_RS27240 0.64 -2.9 5,654,195 - BWI76_RS27240 0.65 -0.2 5,654,223 + BWI76_RS27240 0.68 -0.0 5,654,254 + BWI76_RS27240 0.71 -0.1 5,654,254 + BWI76_RS27240 0.71 +0.1 5,654,278 + BWI76_RS27240 0.74 -0.7 5,654,295 - BWI76_RS27240 0.75 -1.5 5,654,332 - BWI76_RS27240 0.79 -2.9 5,654,568 + +0.4 5,654,571 - -0.4 5,654,588 - -0.7 5,654,621 + BWI76_RS27245 0.13 -0.1 5,654,644 + BWI76_RS27245 0.16 +1.4 5,654,673 - BWI76_RS27245 0.19 +1.1 5,654,799 + BWI76_RS27245 0.35 +0.1 5,654,805 - BWI76_RS27245 0.36 -0.3 5,654,807 - BWI76_RS27245 0.36 +0.2 5,655,008 - BWI76_RS27245 0.61 +0.3 5,655,024 - BWI76_RS27245 0.63 +1.0 5,655,038 - BWI76_RS27245 0.65 -0.6 5,655,065 + BWI76_RS27245 0.68 -0.5 5,655,075 + BWI76_RS27245 0.70 -0.1 5,655,094 - BWI76_RS27245 0.72 -0.4 5,655,094 - BWI76_RS27245 0.72 +0.9 5,655,142 + BWI76_RS27245 0.78 +0.4 5,655,258 - -0.4 5,655,329 + -0.2 5,655,330 + -0.4 5,655,339 + -0.0 5,655,347 - +1.2 5,655,510 + -1.5
Or see this region's nucleotide sequence