Experiment: KLEB17 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25145 and BWI76_RS25150 overlap by 11 nucleotides BWI76_RS25150 and BWI76_RS25155 overlap by 48 nucleotides BWI76_RS25155 and BWI76_RS25160 are separated by 152 nucleotides BWI76_RS25160 and BWI76_RS25165 are separated by 233 nucleotides
BWI76_RS25145: BWI76_RS25145 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase, at 5,234,420 to 5,235,049
_RS25145
BWI76_RS25150: BWI76_RS25150 - hypothetical protein, at 5,235,039 to 5,235,221
_RS25150
BWI76_RS25155: BWI76_RS25155 - RNA-binding protein, at 5,235,174 to 5,235,467
_RS25155
BWI76_RS25160: BWI76_RS25160 - transcription elongation factor GreA, at 5,235,620 to 5,236,096
_RS25160
BWI76_RS25165: BWI76_RS25165 - serine-type D-Ala-D-Ala carboxypeptidase, at 5,236,330 to 5,237,763
_RS25165
Position (kb)
5235
5236
5237 Strain fitness (log2 ratio)
-2
-1
0
1 at 5235.956 kb on - strand, within BWI76_RS25160 at 5236.079 kb on + strand at 5236.129 kb on + strand at 5236.208 kb on - strand at 5236.375 kb on - strand at 5236.414 kb on - strand at 5236.548 kb on + strand, within BWI76_RS25165 at 5236.617 kb on + strand, within BWI76_RS25165 at 5236.727 kb on + strand, within BWI76_RS25165 at 5236.752 kb on - strand, within BWI76_RS25165 at 5236.868 kb on - strand, within BWI76_RS25165
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 0.1 remove 5,235,956 - BWI76_RS25160 0.70 +1.6 5,236,079 + +1.1 5,236,129 + +0.0 5,236,208 - +0.7 5,236,375 - -0.4 5,236,414 - -1.9 5,236,548 + BWI76_RS25165 0.15 -1.9 5,236,617 + BWI76_RS25165 0.20 -0.3 5,236,727 + BWI76_RS25165 0.28 +0.2 5,236,752 - BWI76_RS25165 0.29 +1.4 5,236,868 - BWI76_RS25165 0.38 +1.0
Or see this region's nucleotide sequence