Experiment: KLEB17 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20300 and BWI76_RS20305 are separated by 217 nucleotides BWI76_RS20305 and BWI76_RS20310 are separated by 3 nucleotides BWI76_RS20310 and BWI76_RS20315 are separated by 56 nucleotides BWI76_RS20315 and BWI76_RS20320 are separated by 41 nucleotides
BWI76_RS20300: BWI76_RS20300 - epimerase, at 4,252,241 to 4,253,134
_RS20300
BWI76_RS20305: BWI76_RS20305 - hypothetical protein, at 4,253,352 to 4,253,606
_RS20305
BWI76_RS20310: BWI76_RS20310 - hypothetical protein, at 4,253,610 to 4,254,176
_RS20310
BWI76_RS20315: BWI76_RS20315 - N-acetyltransferase, at 4,254,233 to 4,254,730
_RS20315
BWI76_RS20320: BWI76_RS20320 - histidine/lysine/arginine/ornithine ABC transporter ATP-binding protein HisP, at 4,254,772 to 4,255,545
_RS20320
Position (kb)
4253
4254
4255 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4253.258 kb on + strand at 4253.270 kb on + strand at 4253.270 kb on + strand at 4253.270 kb on + strand at 4253.333 kb on + strand at 4253.641 kb on - strand at 4254.107 kb on + strand, within BWI76_RS20310 at 4254.107 kb on - strand, within BWI76_RS20310 at 4254.115 kb on - strand, within BWI76_RS20310 at 4254.122 kb on + strand at 4254.354 kb on + strand, within BWI76_RS20315 at 4254.505 kb on - strand, within BWI76_RS20315 at 4254.561 kb on + strand, within BWI76_RS20315 at 4254.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 0.1 remove 4,253,258 + -0.9 4,253,270 + -4.1 4,253,270 + -0.9 4,253,270 + -0.4 4,253,333 + -1.2 4,253,641 - -3.2 4,254,107 + BWI76_RS20310 0.88 -1.3 4,254,107 - BWI76_RS20310 0.88 -0.4 4,254,115 - BWI76_RS20310 0.89 -2.3 4,254,122 + +0.1 4,254,354 + BWI76_RS20315 0.24 +0.0 4,254,505 - BWI76_RS20315 0.55 -0.7 4,254,561 + BWI76_RS20315 0.66 -0.7 4,254,777 - +0.3
Or see this region's nucleotide sequence