Experiment: KLEB17 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03465 and BWI76_RS03470 are separated by 66 nucleotides BWI76_RS03470 and BWI76_RS03475 are separated by 339 nucleotides BWI76_RS03475 and BWI76_RS03480 are separated by 53 nucleotides
BWI76_RS03465: BWI76_RS03465 - 5'/3'-nucleotidase SurE, at 715,467 to 716,342
_RS03465
BWI76_RS03470: BWI76_RS03470 - 50S ribosomal protein L16 arginine hydroxylase, at 716,409 to 717,533
_RS03470
BWI76_RS03475: BWI76_RS03475 - hypothetical protein, at 717,873 to 718,078
_RS03475
BWI76_RS03480: BWI76_RS03480 - 16S ribosomal RNA, at 718,132 to 719,685
_RS03480
Position (kb)
716
717
718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 715.417 kb on - strand at 715.453 kb on + strand at 715.457 kb on - strand at 715.474 kb on + strand at 715.507 kb on - strand at 715.561 kb on - strand, within BWI76_RS03465 at 715.611 kb on + strand, within BWI76_RS03465 at 715.617 kb on + strand, within BWI76_RS03465 at 715.624 kb on + strand, within BWI76_RS03465 at 715.625 kb on + strand, within BWI76_RS03465 at 715.625 kb on + strand, within BWI76_RS03465 at 715.625 kb on + strand, within BWI76_RS03465 at 715.625 kb on - strand, within BWI76_RS03465 at 715.659 kb on + strand, within BWI76_RS03465 at 715.680 kb on + strand, within BWI76_RS03465 at 715.686 kb on - strand, within BWI76_RS03465 at 715.686 kb on - strand, within BWI76_RS03465 at 715.733 kb on - strand, within BWI76_RS03465 at 715.762 kb on + strand, within BWI76_RS03465 at 715.850 kb on + strand, within BWI76_RS03465 at 715.850 kb on + strand, within BWI76_RS03465 at 715.858 kb on - strand, within BWI76_RS03465 at 715.971 kb on + strand, within BWI76_RS03465 at 715.971 kb on + strand, within BWI76_RS03465 at 716.029 kb on - strand at 716.065 kb on + strand at 716.065 kb on + strand, within BWI76_RS03465 at 716.073 kb on - strand, within BWI76_RS03465 at 716.073 kb on - strand, within BWI76_RS03465 at 716.141 kb on + strand, within BWI76_RS03465 at 716.141 kb on + strand, within BWI76_RS03465 at 716.144 kb on - strand, within BWI76_RS03465 at 716.146 kb on + strand, within BWI76_RS03465 at 716.161 kb on + strand, within BWI76_RS03465 at 716.161 kb on + strand, within BWI76_RS03465 at 716.161 kb on + strand, within BWI76_RS03465 at 716.161 kb on + strand, within BWI76_RS03465 at 716.169 kb on - strand, within BWI76_RS03465 at 716.169 kb on - strand, within BWI76_RS03465 at 716.169 kb on - strand, within BWI76_RS03465 at 716.185 kb on + strand, within BWI76_RS03465 at 716.193 kb on - strand, within BWI76_RS03465 at 716.229 kb on + strand, within BWI76_RS03465 at 716.277 kb on - strand at 716.279 kb on + strand at 716.306 kb on + strand at 716.306 kb on + strand at 716.314 kb on - strand at 716.326 kb on - strand at 716.326 kb on - strand at 716.328 kb on - strand at 716.389 kb on + strand at 716.432 kb on - strand at 716.470 kb on + strand at 716.489 kb on + strand at 716.500 kb on + strand at 716.502 kb on - strand at 716.506 kb on - strand at 716.508 kb on - strand at 716.508 kb on - strand at 716.508 kb on - strand at 716.513 kb on + strand at 716.513 kb on + strand at 716.518 kb on - strand at 716.518 kb on - strand at 716.521 kb on - strand at 716.521 kb on - strand at 716.526 kb on - strand, within BWI76_RS03470 at 716.666 kb on - strand, within BWI76_RS03470 at 716.726 kb on + strand, within BWI76_RS03470 at 716.733 kb on + strand, within BWI76_RS03470 at 716.802 kb on - strand, within BWI76_RS03470 at 716.804 kb on + strand, within BWI76_RS03470 at 716.856 kb on - strand, within BWI76_RS03470 at 716.888 kb on + strand, within BWI76_RS03470 at 716.933 kb on - strand, within BWI76_RS03470 at 716.938 kb on + strand, within BWI76_RS03470 at 716.946 kb on - strand, within BWI76_RS03470 at 717.042 kb on - strand, within BWI76_RS03470 at 717.049 kb on + strand, within BWI76_RS03470 at 717.057 kb on - strand, within BWI76_RS03470 at 717.070 kb on + strand, within BWI76_RS03470 at 717.142 kb on + strand, within BWI76_RS03470 at 717.248 kb on + strand, within BWI76_RS03470 at 717.248 kb on + strand, within BWI76_RS03470 at 717.256 kb on - strand, within BWI76_RS03470 at 717.260 kb on + strand, within BWI76_RS03470 at 717.293 kb on - strand, within BWI76_RS03470 at 717.350 kb on + strand, within BWI76_RS03470 at 717.405 kb on - strand, within BWI76_RS03470 at 717.529 kb on + strand at 717.529 kb on + strand at 717.581 kb on + strand at 717.589 kb on - strand at 717.596 kb on + strand at 717.602 kb on + strand at 717.602 kb on + strand at 717.610 kb on - strand at 717.610 kb on - strand at 717.653 kb on - strand at 717.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 0.1 remove 715,417 - -0.7 715,453 + -0.2 715,457 - +0.7 715,474 + +1.2 715,507 - +2.2 715,561 - BWI76_RS03465 0.11 -0.2 715,611 + BWI76_RS03465 0.16 +0.3 715,617 + BWI76_RS03465 0.17 +0.5 715,624 + BWI76_RS03465 0.18 -3.0 715,625 + BWI76_RS03465 0.18 +1.0 715,625 + BWI76_RS03465 0.18 -0.3 715,625 + BWI76_RS03465 0.18 -0.2 715,625 - BWI76_RS03465 0.18 +0.6 715,659 + BWI76_RS03465 0.22 -0.3 715,680 + BWI76_RS03465 0.24 -0.2 715,686 - BWI76_RS03465 0.25 +0.5 715,686 - BWI76_RS03465 0.25 +0.8 715,733 - BWI76_RS03465 0.30 -0.3 715,762 + BWI76_RS03465 0.34 +0.8 715,850 + BWI76_RS03465 0.44 -0.1 715,850 + BWI76_RS03465 0.44 -0.4 715,858 - BWI76_RS03465 0.45 +1.3 715,971 + BWI76_RS03465 0.58 -0.1 715,971 + BWI76_RS03465 0.58 -1.2 716,029 - +2.3 716,065 + -1.0 716,065 + BWI76_RS03465 0.68 +1.5 716,073 - BWI76_RS03465 0.69 +0.4 716,073 - BWI76_RS03465 0.69 -0.0 716,141 + BWI76_RS03465 0.77 +0.4 716,141 + BWI76_RS03465 0.77 +0.0 716,144 - BWI76_RS03465 0.77 -0.2 716,146 + BWI76_RS03465 0.78 +0.0 716,161 + BWI76_RS03465 0.79 -0.3 716,161 + BWI76_RS03465 0.79 +0.4 716,161 + BWI76_RS03465 0.79 +0.2 716,161 + BWI76_RS03465 0.79 +0.0 716,169 - BWI76_RS03465 0.80 +0.9 716,169 - BWI76_RS03465 0.80 +0.1 716,169 - BWI76_RS03465 0.80 +0.4 716,185 + BWI76_RS03465 0.82 +0.4 716,193 - BWI76_RS03465 0.83 +0.5 716,229 + BWI76_RS03465 0.87 +0.4 716,277 - -2.7 716,279 + +1.6 716,306 + +0.8 716,306 + -0.4 716,314 - +0.6 716,326 - -1.2 716,326 - -0.2 716,328 - +1.1 716,389 + -0.3 716,432 - +1.0 716,470 + -1.9 716,489 + +0.1 716,500 + +0.5 716,502 - +0.2 716,506 - -0.1 716,508 - -1.5 716,508 - +0.2 716,508 - -1.1 716,513 + +0.6 716,513 + -0.1 716,518 - +0.2 716,518 - +0.3 716,521 - +1.1 716,521 - +1.5 716,526 - BWI76_RS03470 0.10 -0.5 716,666 - BWI76_RS03470 0.23 +2.8 716,726 + BWI76_RS03470 0.28 -0.5 716,733 + BWI76_RS03470 0.29 -0.0 716,802 - BWI76_RS03470 0.35 +0.7 716,804 + BWI76_RS03470 0.35 -0.4 716,856 - BWI76_RS03470 0.40 -1.3 716,888 + BWI76_RS03470 0.43 +0.8 716,933 - BWI76_RS03470 0.47 +0.2 716,938 + BWI76_RS03470 0.47 +0.4 716,946 - BWI76_RS03470 0.48 +1.1 717,042 - BWI76_RS03470 0.56 +0.6 717,049 + BWI76_RS03470 0.57 +0.7 717,057 - BWI76_RS03470 0.58 -0.1 717,070 + BWI76_RS03470 0.59 +0.4 717,142 + BWI76_RS03470 0.65 +0.5 717,248 + BWI76_RS03470 0.75 +0.8 717,248 + BWI76_RS03470 0.75 +0.3 717,256 - BWI76_RS03470 0.75 -2.3 717,260 + BWI76_RS03470 0.76 +0.2 717,293 - BWI76_RS03470 0.79 -1.2 717,350 + BWI76_RS03470 0.84 -0.1 717,405 - BWI76_RS03470 0.89 -0.3 717,529 + +1.9 717,529 + +0.9 717,581 + -1.6 717,589 - -0.1 717,596 + -0.1 717,602 + +0.5 717,602 + +0.4 717,610 - +0.5 717,610 - +1.1 717,653 - -0.1 717,866 - +1.0
Or see this region's nucleotide sequence