Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25590 and BWI76_RS25595 are separated by 226 nucleotides BWI76_RS25595 and BWI76_RS25600 are separated by 39 nucleotides BWI76_RS25600 and BWI76_RS25605 are separated by 14 nucleotides BWI76_RS25605 and BWI76_RS25610 are separated by 56 nucleotides BWI76_RS25610 and BWI76_RS25615 are separated by 181 nucleotides BWI76_RS25615 and BWI76_RS25620 are separated by 77 nucleotides
BWI76_RS25590: BWI76_RS25590 - membrane protein, at 5,319,713 to 5,319,934
_RS25590
BWI76_RS25595: BWI76_RS25595 - 5S ribosomal RNA, at 5,320,161 to 5,320,276
_RS25595
BWI76_RS25600: BWI76_RS25600 - tRNA-Thr, at 5,320,316 to 5,320,391
_RS25600
BWI76_RS25605: BWI76_RS25605 - 5S ribosomal RNA, at 5,320,406 to 5,320,521
_RS25605
BWI76_RS25610: BWI76_RS25610 - 23S ribosomal RNA, at 5,320,578 to 5,323,505
_RS25610
BWI76_RS25615: BWI76_RS25615 - tRNA-Glu, at 5,323,687 to 5,323,761
_RS25615
BWI76_RS25620: BWI76_RS25620 - 16S ribosomal RNA, at 5,323,839 to 5,325,392
_RS25620
Position (kb)
5320
5321
5322
5323
5324 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5319.629 kb on + strand at 5319.634 kb on - strand at 5319.637 kb on - strand at 5319.639 kb on - strand at 5319.656 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.664 kb on + strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.672 kb on - strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.675 kb on + strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.683 kb on - strand at 5319.700 kb on - strand at 5319.742 kb on + strand, within BWI76_RS25590 at 5319.754 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.764 kb on + strand, within BWI76_RS25590 at 5319.772 kb on - strand, within BWI76_RS25590 at 5319.774 kb on + strand, within BWI76_RS25590 at 5319.782 kb on - strand, within BWI76_RS25590 at 5319.812 kb on + strand, within BWI76_RS25590 at 5319.817 kb on + strand, within BWI76_RS25590 at 5319.817 kb on + strand, within BWI76_RS25590 at 5319.826 kb on - strand, within BWI76_RS25590 at 5319.850 kb on - strand, within BWI76_RS25590 at 5319.995 kb on + strand at 5320.004 kb on - strand at 5320.019 kb on - strand at 5320.353 kb on - strand, within BWI76_RS25600
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 5,319,629 + +0.2 5,319,634 - +0.1 5,319,637 - -0.9 5,319,639 - -1.2 5,319,656 + +0.9 5,319,664 + +1.6 5,319,664 + -1.6 5,319,664 + -0.1 5,319,664 + +2.9 5,319,664 + +1.0 5,319,664 + +0.7 5,319,664 + +0.7 5,319,664 + +0.5 5,319,672 - -1.8 5,319,672 - -2.0 5,319,672 - -1.1 5,319,672 - +2.3 5,319,672 - +0.1 5,319,672 - +1.9 5,319,672 - -0.5 5,319,672 - -3.5 5,319,672 - +0.4 5,319,672 - -2.0 5,319,672 - -1.7 5,319,675 + +0.3 5,319,675 + -0.5 5,319,675 + +0.4 5,319,675 + +0.3 5,319,675 + -0.5 5,319,675 + +1.0 5,319,675 + +0.0 5,319,675 + -0.7 5,319,683 - +1.5 5,319,683 - +2.2 5,319,683 - +0.5 5,319,683 - +0.5 5,319,683 - +0.2 5,319,683 - -0.2 5,319,683 - +0.8 5,319,700 - -1.0 5,319,742 + BWI76_RS25590 0.13 +1.0 5,319,754 + BWI76_RS25590 0.18 -0.9 5,319,764 + BWI76_RS25590 0.23 +1.6 5,319,764 + BWI76_RS25590 0.23 +0.6 5,319,764 + BWI76_RS25590 0.23 +2.2 5,319,764 + BWI76_RS25590 0.23 +2.4 5,319,772 - BWI76_RS25590 0.27 -0.1 5,319,774 + BWI76_RS25590 0.27 +0.9 5,319,782 - BWI76_RS25590 0.31 +0.3 5,319,812 + BWI76_RS25590 0.45 -0.9 5,319,817 + BWI76_RS25590 0.47 -0.2 5,319,817 + BWI76_RS25590 0.47 +0.8 5,319,826 - BWI76_RS25590 0.51 -1.4 5,319,850 - BWI76_RS25590 0.62 +0.0 5,319,995 + +1.4 5,320,004 - -1.0 5,320,019 - -0.1 5,320,353 - BWI76_RS25600 0.49 -3.3
Or see this region's nucleotide sequence