Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24305 and BWI76_RS24310 are separated by 94 nucleotides BWI76_RS24310 and BWI76_RS24315 are separated by 120 nucleotides
BWI76_RS24305: BWI76_RS24305 - 2,5-didehydrogluconate reductase A, at 5,063,725 to 5,064,552
_RS24305
BWI76_RS24310: BWI76_RS24310 - YgiQ family radical SAM protein, at 5,064,647 to 5,066,830
_RS24310
BWI76_RS24315: BWI76_RS24315 - cell division protein FtsP, at 5,066,951 to 5,068,363
_RS24315
Position (kb) 5064
5065
5066
5067 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5063.666 kb on + strand at 5063.674 kb on - strand at 5063.710 kb on - strand at 5063.763 kb on + strand at 5063.783 kb on - strand at 5063.910 kb on - strand, within BWI76_RS24305 at 5063.912 kb on + strand, within BWI76_RS24305 at 5063.920 kb on - strand, within BWI76_RS24305 at 5063.976 kb on + strand, within BWI76_RS24305 at 5063.976 kb on + strand, within BWI76_RS24305 at 5064.083 kb on + strand, within BWI76_RS24305 at 5064.307 kb on + strand, within BWI76_RS24305 at 5064.385 kb on + strand, within BWI76_RS24305 at 5064.385 kb on + strand, within BWI76_RS24305 at 5064.385 kb on + strand, within BWI76_RS24305 at 5064.510 kb on + strand at 5064.906 kb on + strand, within BWI76_RS24310 at 5064.959 kb on + strand, within BWI76_RS24310 at 5064.969 kb on + strand, within BWI76_RS24310 at 5064.977 kb on - strand, within BWI76_RS24310 at 5064.993 kb on + strand, within BWI76_RS24310 at 5065.165 kb on + strand, within BWI76_RS24310 at 5065.345 kb on + strand, within BWI76_RS24310 at 5065.483 kb on - strand, within BWI76_RS24310 at 5065.517 kb on - strand, within BWI76_RS24310 at 5065.816 kb on + strand, within BWI76_RS24310 at 5065.925 kb on + strand, within BWI76_RS24310 at 5065.954 kb on + strand, within BWI76_RS24310 at 5066.077 kb on + strand, within BWI76_RS24310 at 5066.173 kb on - strand, within BWI76_RS24310 at 5066.400 kb on - strand, within BWI76_RS24310 at 5066.405 kb on - strand, within BWI76_RS24310 at 5066.479 kb on - strand, within BWI76_RS24310 at 5066.546 kb on + strand, within BWI76_RS24310 at 5066.561 kb on - strand, within BWI76_RS24310 at 5066.924 kb on + strand at 5066.939 kb on + strand at 5066.939 kb on + strand at 5066.985 kb on - strand at 5067.060 kb on - strand at 5067.255 kb on + strand, within BWI76_RS24315 at 5067.263 kb on - strand at 5067.554 kb on - strand, within BWI76_RS24315 at 5067.656 kb on - strand, within BWI76_RS24315 at 5067.767 kb on + strand, within BWI76_RS24315 at 5067.775 kb on - strand, within BWI76_RS24315
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 5,063,666 + +0.8 5,063,674 - +1.1 5,063,710 - -1.8 5,063,763 + +0.1 5,063,783 - -3.6 5,063,910 - BWI76_RS24305 0.22 +0.2 5,063,912 + BWI76_RS24305 0.23 -0.5 5,063,920 - BWI76_RS24305 0.24 -2.5 5,063,976 + BWI76_RS24305 0.30 -2.1 5,063,976 + BWI76_RS24305 0.30 -0.3 5,064,083 + BWI76_RS24305 0.43 -1.1 5,064,307 + BWI76_RS24305 0.70 -0.2 5,064,385 + BWI76_RS24305 0.80 +1.6 5,064,385 + BWI76_RS24305 0.80 -3.4 5,064,385 + BWI76_RS24305 0.80 -0.8 5,064,510 + -0.8 5,064,906 + BWI76_RS24310 0.12 -1.3 5,064,959 + BWI76_RS24310 0.14 +0.1 5,064,969 + BWI76_RS24310 0.15 +1.7 5,064,977 - BWI76_RS24310 0.15 -0.0 5,064,993 + BWI76_RS24310 0.16 -0.4 5,065,165 + BWI76_RS24310 0.24 -0.1 5,065,345 + BWI76_RS24310 0.32 +0.2 5,065,483 - BWI76_RS24310 0.38 +0.4 5,065,517 - BWI76_RS24310 0.40 -1.8 5,065,816 + BWI76_RS24310 0.54 +1.6 5,065,925 + BWI76_RS24310 0.59 +1.6 5,065,954 + BWI76_RS24310 0.60 -1.8 5,066,077 + BWI76_RS24310 0.65 +2.0 5,066,173 - BWI76_RS24310 0.70 +1.1 5,066,400 - BWI76_RS24310 0.80 -0.4 5,066,405 - BWI76_RS24310 0.80 +4.3 5,066,479 - BWI76_RS24310 0.84 +0.3 5,066,546 + BWI76_RS24310 0.87 -0.0 5,066,561 - BWI76_RS24310 0.88 +0.8 5,066,924 + +1.0 5,066,939 + -0.4 5,066,939 + +0.7 5,066,985 - -4.1 5,067,060 - -1.2 5,067,255 + BWI76_RS24315 0.22 -0.0 5,067,263 - -2.1 5,067,554 - BWI76_RS24315 0.43 +0.4 5,067,656 - BWI76_RS24315 0.50 -0.1 5,067,767 + BWI76_RS24315 0.58 -0.7 5,067,775 - BWI76_RS24315 0.58 -1.4
Or see this region's nucleotide sequence